Comparative study of seminal plasma proteins in dromedary camels

GORAKH MAL; SUMANT VYAS; N V PATIL

doi:10.56093/ijans.v84i1.37291

Authors

GORAKH MAL Indian Veterinary Research Institute, Regional Station, Palampur, Himachal Pradesh
SUMANT VYAS National Research Centre on Camel, Bikaner, Rajasthan 334 001 India
N V PATIL National Research Centre on Camel, Bikaner, Rajasthan 334 001 India

https://doi.org/10.56093/ijans.v84i1.37291

Keywords:

Jaisalmeri camel, Seminal plasma protein, SDS-PAGE, Total protein

Abstract

Semen ejaculates (30) were obtained from 5 Jaisalmeri camels. Fresh semen samples were centrifuged at 6,000 rpm for 30 min and clear supernatant of seminal plasma was separated and stored at –20°C until used. Total protein from the seminal plasma was estimated and the molecular weight was analysed. The total protein concentration was between 2.28–2.59 g/dl in the seminal plasma. In the seminal plasma of 4 camels, 11 protein bands were observed ranging from 11.7–104.0 kDa, whereas, 9 protein bands were observed in the seminal plasma of 1 camel. The present study showed similar seminal plasma protein profile in Jaisalmeri camels except 2 protein fractions of 40kDa and 12.9kDa were expressed in 4 camels and not in 1 camel. It can be concluded that at least 9 protein fractions were in common between these camels.

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