In ovo injection effect of sodium nitrite on hatchability, day-old chick quality, organ weights and body antioxidant status of newly hatched chicks

ELMIRA MOHAMADI; MOHSEN DANESHYAR; FARHAD FAROKHI ARDABILI

doi:10.56093/ijans.v84i1.37294

Authors

ELMIRA MOHAMADI Urmia University, Urmia, Iran
MOHSEN DANESHYAR Urmia University, Urmia, Iran
FARHAD FAROKHI ARDABILI Urmia University, Urmia, Iran

https://doi.org/10.56093/ijans.v84i1.37294

Keywords:

Blood metabolites, Chick quality, Malondialdehyde, Nitrite, Total antioxidant capacity

Abstract

This experiment was conducted to investigate the effect of sodium nitrite injection per egg on day 7 of incubation on chick quality, hatchability, chick weights and some blood antioxidant metabolites in chicken embryo. Fertile eggs (308) were injected by 4 different levels of 0, 0.042, 0.084 and 0.168 mg per egg sodium nitrite at day 7 of incubation. At hatch, no effect of sodium nitrite injection was indicated on hatchability and mortality, chick quality and organ weights. However chick weight of sodium nitrite injected eggs were lower than those of control chicks. No significant differences were observed between the treatments for activity of blood superoxide dismutase and glutathione peroxidase. Blood malondialdehyde content of chicks from 0.084 mg sodium nitrite injected eggs was greater than control and 0.042 mg sodium nitrite injected ones. Furthermore, lower blood total antioxidant capacity was observed for chicks of 0.042 and 0.168 mg sodium nitrite injected eggs as compared to those of control eggs. It was concluded that In ovo injection of sodium nitrite does not exert negative effects on 1–day chick quality, hatchability and mortality, but it decreased the both chick weights and blood antioxidant status.

Downloads

Download data is not yet available.

References

Daneshyar M, Geuns M C J, Buyse G J, Willemsen H, Decuypere E, Everaert N, Kermanshahi H and Ansari Z. 2010. Evaluation of steviol injection on chicken embryos: Effects on post-hatch development, proportional organ weights, plasma thyroid hormons and metabolites. Journal of Poultry Science 47: 71– 76.

Fan A M, Willhite C C and Book S A. 1987. Evaluation of the nitrate drinking water standard with reference to infant methemoglobinemia and potential reproductive toxicity. Regulatory Toxicology and Pharmacolology 7: 135–48.

Fan A M and Steinberg V E. 1996. Health implications of nitrate and nitrite in drinking water: an update on methemoglobinemia occurrence and reproductive and developmental toxicity. Regulatory Toxicology and Pharmacolology 23: 35–43.

Gladwin M T, Crawford J H and Patel R P. 2004. The biochemistry of nitric oxide, nitrite, and hemoglobin: role in blood flow regulation. Free Radical Biology and Medicine 36: 707– 17.

Ger J, Kao H, Shih T S and Deng J F. 1996. Fatal toxic methemoglobinemia due to occupational exposure to methyl nitrite. Clinical Medicine Journal 57: S78.

Globus M and Samuel D. 1978. Effect of maternally administered sodium nitrite on hepatic erythropoiesis in fetal CD–1 mice. Teratology 18: 367–78.

Gruener N, Shuval H I, Behroozi K, Cohen S and Shechte R H. 1973. Methemoglobinemia induced by trans placental passage of nitrites in rats. Bulletin Environmental Contamination and Toxicology 9: 44–48.

Gupta R C, Gupta A B, Seth A K, Bassin J K and Gupta A. 2000. Recurrent acute respiratory infections in areas with high nitrate concentrations in drinking water. Environmental Health Perspectives 108: 363–66.

Hamburger V and Hamilton H L. 1951. A series of normal stages in the ability development of the chick embryo. Journal of Morphology 88: 49–92.

Hill M J. 1999. Nitrate toxicity: myth or reality? British Journal of Nutrition 81: 343–44.

Horakova L, Stole S, Chromikova Z, Pekarova A and Derkova L. 1998. Mechanisms of hippocampal reoxygenation injury. Molecular and Chemical Neuropathology 33: 223–36.

Inui N, Nishi Y, Taketomi M and Mori M. 1979. Trans placentalaction of sodium nitrite on embryonic cells of Syrian golden hamster. Mutatation Research 66: 149–58.

Kagan V E. 1988. Lipid Peroxidation in Biomembranes. Boca. Raton, FL, CRC.

Kroupova H, Prokes M, Macova S, Penaz M, Barus V, Novotny L and Machova J. 2010. Effect of nitrite on early-life stages of common crap (Cyprinus carpio L.). Environmental Toxicology Chemistry 29: 535–40.

Meerson F Z, Kagan V E, Kozlov Y P, Belkina L M and Arkhipenko Y V. 1982. The role of lipid peroxidation in pathogenesis of ischemic damage and the antioxidant protection of the heart. Basic Research in Cardiology 77: 465–85.

Padmaja S, Squadrito G L and Pryor W A. 1998. Inactivation of glutathione peroxidase by peroxy nitrite. Archives of Biochemistry and Biophysics 349: 1–6.

Page R D, Gilson W D, Guthri E D, Mertens D R and Hatch R C. 1990. Serum progesterone and milk production and composition in dairy cows fed two concentrations of nitrate. Veterinary and Human Toxicolology 32: 27–31.

Row B W, Kheirandish L, Neville J J and Gozal D. 2002. Impaired spatial learning and hyper activity in developing rats exposed to intermittent hypoxia. Pediatric Research 52: 449–53.

SAS Institute. 2002. SAS Users Guide: Statistics. SAS Institute Inc., Cary, NC.

Tona K, Bamelis F, De Ketelaere B, Bruggeman V, Moraes V M B, Buyse J, Onagbesan O, Winter A J and Hokanson J F. 1964. Effects of long-term feeding of nitrate, nitrite, or hydroxylamine on pregnant dairy heifers. American Journal of Veterinary Research 25: 353–61.

Willier B H. 1954. Phases in embryonic development. Journal of Cellular and Comparative Physiology 43: 307–17.

Yoshikawa T Y, Furukawa S, Wakamatsu H, Takemura H and Kondo M. 1982. Experimental hypoxia and lipid peroxidation in rats.

Biochemical Medicine 27: 207–13.