Effect of GDF-9 and bFGF in combination on caprine granulosa cell growth parameters in vitro

U S PAVANA SHREE; S NANDI; V GIRISH KUMAR; P S P GUPTA; V CHANDRASEKHAR MURTHY

doi:10.56093/ijans.v84i1.37298

Authors

U S PAVANA SHREE National Institute of Animal Nutrition and physiology, Adugodi, Bangalore, Karnataka 560 030 India
S NANDI National Institute of Animal Nutrition and physiology, Adugodi, Bangalore, Karnataka 560 030 India
V GIRISH KUMAR National Institute of Animal Nutrition and physiology, Adugodi, Bangalore, Karnataka 560 030 India
P S P GUPTA National Institute of Animal Nutrition and physiology, Adugodi, Bangalore, Karnataka 560 030 India
V CHANDRASEKHAR MURTHY National Institute of Animal Nutrition and physiology, Adugodi, Bangalore, Karnataka 560 030 India

https://doi.org/10.56093/ijans.v84i1.37298

Abstract

The present study was undertaken to examine the effect of GDF-9 and bFGF on in vitro growth of granulosa cells in goats. Granulosa cell number increment and monolayer formation score were significantly higher in media containing GDF-9 and bFGF at 20 ng/ml. No significant difference was observed in viability, MTT oxidation and ability of granulosa cell monolayer to support oocyte maturation between control and treatment groups. In conclusions, GDF-9 and FGF at 20 ng/ml improved granulosa cell functions and oocyte maturation indicating its influence on the mechanism of oocyte growth.

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