New genetic polymorphisms in Indian goat BMPR1B gene

SONIKA AHLAWAT; REKHA SHARMA; A MAITRA; M S TANTIA; MANORANJAN ROY; S MANDAKMALE

doi:10.56093/ijans.v84i1.37303

Authors

SONIKA AHLAWAT National Bureau of Animal Genetic Resources, Karnal, Haryana 132 001 India
REKHA SHARMA National Bureau of Animal Genetic Resources, Karnal, Haryana 132 001 India
A MAITRA National Bureau of Animal Genetic Resources, Karnal, Haryana 132 001 India
M S TANTIA National Bureau of Animal Genetic Resources, Karnal, Haryana 132 001 India
MANORANJAN ROY National Bureau of Animal Genetic Resources, Karnal, Haryana 132 001 India
S MANDAKMALE Mahatma Phule Krishi Vidyapeeth, Rahuri 413 722

https://doi.org/10.56093/ijans.v84i1.37303

Keywords:

BMPR1B, Indian goat, Polymorphism, Promoter, SNPs

Abstract

Studies on the genetics of prolificacy in small ruminants have highlighted the importance of BMPR1B (bone morphogenetic protein receptor IB) gene in affecting ovulation rate and litter size. Most of the research on BMPR1B gene has revolved around screening sheep and goats for the presence of FecB mutation (A746G) that is associated with hyperprolific phenotype in many breeds around the world. In the present study, an attempt was made to detect polymorphism in the promoter and exonic regions (exon1, 2 and 6 to 9) of BMPR1B gene in 8 indigenous goat breeds (Barbari, Beetal, Black Bengal, Malabari, Jakhrana, Osmanabadi, Sirohi and Ganjam) varying in prolificacy and geographic distribution. Targeted DNA fragments (2612 bp) were obtained through PCR and DNA sequencing methods. BMPR1B promoter region was amplified in two fragments and a 1032 bp length contig was generated for the first time for goats. Two novel SNPs T(-242)C and G(–623)A were identified in the promoter region of caprine BMPR1B gene by sequence alignment. Hundred variations were observed between cattle and goat promoter sequence. The variations did not change or affect any transcription binding sites in promoter of BMPR1B gene. Sequence analysis revealed absence of any polymorphism including the FecB mutation in exonic regions of all the investigated breeds of Indian goats. This is the first report of identification of mutation in regulatory region of BMPR1B gene, which may be helpful for better understanding of the role played by this gene in reproduction of the goat.

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References

Acharya R M. 1982. Sheep and Goat Breed of India. FAO Animal Production and Health Paper. pp. 1–190. Rome: FAO, United Nations.

Ahlawat S, Sharma R and Maitra A. 2012a. Analysis of coding DNA sequence of GDF9 gene in Indian goats for prolificacy associated markers. Indian Journal of Animal Sciences 82(7): 721–25.

Ahlawat S, Sharma R and Maitra A. 2013b. Screening of indigenous goats for prolificacy associated DNA markers of sheep. Gene 517:128–31.

Chu M X, Zhao X H, Zhang Y J, Jin M, Wang J Y, Di R, Cao G L, Feng T, Fang L, Ma Y H, Li K. 2010. Polymorphisms of BMPRIB gene and their relationship with litter size in goats. Molecular Biology Reports 37: 4033–39.

Davis G H, Montgomery G W, Allison A J, Kelly R W and Bray A R. 1982. Segregation of a major gene influencing fecundity in progeny of Booroola sheep. New Zealand Journal of Agricultural Research 25: 525–29.

Feng T, Geng C X, Lang X Z, Chu M X, Cao G L and Fang L, Chen H Q, Liu X L and Li N. 2010. Polymorphisms of caprine GDF9 gene and their association with litter size in Jining Grey goats. Molecular Biology Reports. DOI 10.1007/s11033–010– 0669-y, 2010.

He Y, Ma X, Liu X, Zhang C and Li J. 2010. Candidate genes polymorphism and its association to prolificacy in Chinese Goats. Journal of Agricultural Science 2: 88–92.

Hua G H, Chen S L, Ai J T and Yang L G. 2008. None of the polymorphism of ovine fecundity major genes FecB and FecX was tested in goat. Animal Reproduction Science 108: 279–86.

Kel A E, Gössling E, Reuter I, Cheremushkin E, Kel-Margoulis O V and Wingender E. 2003. MATCH: A tool for searching transcription factor binding sites in DNA sequences. Nucleic Acids Research 31(13): 3576–79.

Livestock Census. 2007. Directorate of Economics and Statistics, and Animal Husbandry Statistics Division, Department of Animal Husbandry, Dairying and Fisheries, M/O Agriculture. Polley S, De S, Batabyal S, Kaushik R, Yadav P and Arora J S, Chattopadhyay S, Pan S, Brahma B, Datta T K and Goswami S L. 2009. Polymorphism of fecundity genes (BMPR1B, BMP15 and GDF9) in the Indian prolific Black Bengal goat. Small Ruminant Research 85: 122–29.

Sambrook J and Russell D W. 2001. Molecular Cloning: A Laboratory Manuel III. Cold Spring Harbour, Cold Spring Laboratory Press, NY, USA.

Souza C J, MacDougall C, Campbell B K, McNeilly A S and Baird D T. 2001. The Booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type 1B (BMPRIB) gene. Journal of Endocrinology 169(2): R1–R6.

Supakorn C and Pralomkarn W. 2010. Sheep FecB Gene Polymorphism Role in Thai Meat Goat Proliferation Rate. Proceedings of the 9th World Congress Genetics Applied to Livestock Production. 2–6 August 2010, Leipiz Germany.

Zeshmarani S, Dhara K C, Samanta A K, Samanta R and Majumder S C. 2007. Reproductive performance of goats in Eastern and North-eastern India. Livestock Research for Rural Development 19. Article no.114.