Cloning of partial glycoprotein B gene and molecular epidemiological studies of bovine herpes virus -1 isolates

B M CHANDRANAIK; D RATHNAMMA; S S PATIL; S RANGANATHA; RAMESH C KOVI; D S AKHILA; SHRIKRISHNA ISLOOR; C RENUKAPRASAD; K PRABHUDAS

doi:10.56093/ijans.v84i2.37811

Authors

B M CHANDRANAIK Animal and Fisheries Sciences University, Bidar, Karnataka 585 401 India
D RATHNAMMA Animal and Fisheries Sciences University, Bidar, Karnataka 585 401 India
S S PATIL Animal and Fisheries Sciences University, Bidar, Karnataka 585 401 India
S RANGANATHA Animal and Fisheries Sciences University, Bidar, Karnataka 585 401 India
RAMESH C KOVI University of Minnesota, St. Paul, Minnesota, USA
D S AKHILA Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, Karnataka 585 401 India
SHRIKRISHNA ISLOOR Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, Karnataka 585 401 India
C RENUKAPRASAD Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, Karnataka 585 401 India
K PRABHUDAS Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, Karnataka 585 401 India

https://doi.org/10.56093/ijans.v84i2.37811

Keywords:

Bovine herpes virus-1, Clinical relevance, Genogrouping, Molecular epidemiology, Serology, Virus isolation

Abstract

The study describes genetic grouping and molecular epidemiology of bovine herpes virus-1 (BoHV-1) through cloning of partial glycoprotein B gene of BoHV-1 isolates, with special reference to isolates recovered from cattle breeding stations. Samples were collected from 212 animals (91 bulls and 121 female cattle). Avidin-biotin ELISA employed on serum samples found 74 animals as seropositive for BoHV-1. On inoculation of 212 semen/swab samples to MDBK cell line for virus isolation, samples of 4 seropositive and 5 seronegative animals yielded cytopathic changes characteristic of BoHV-1. Partial gB gene of these isolates were cloned in pGEM T vector, nucleotide sequences were deduced and phylogenetic tree was constructed. Sequence analysis grouped 5 of these isolates under BoHV-1.1 cluster having highest sequence identities with previously described Indian, European and Brazilian isolates of BoHV-1.1. The other 4 isolates were clustered as BoHV-1.2 subtypes having 100% sequence identity with European strain of BoHV-1.2. We found that, apparently healthy, seronegative animals can be sources of BoHV-1, attributable to the unique pathogenesis/ latency of BoHV-1. This finding necessitates mandatory culling of breeding animals which are positive either in antigen detection or by serology, especially in countries which do not practice vaccination but report high seroprevalance of BoHV-1.

Downloads

Download data is not yet available.

References

Ackermann M and Engels M. 2006. Pro and contra IBR eradication. Veterinary Microbiology 11: 293–302.

Anonymous. 2010. Infectious Bovine Rhinotracheitis. Manual of Diagnostic Tests and Vaccines for Terrestrial Animal P: 752– 63.

Antinone S E, Shubeita G T, Coller K E, Lee J I, Haverlock-Moyns S, Gross S P and Smith GA. 2006. The Herpesvirus capsid surface protein, VP26, and the majority of the tegument proteins are dispensable for capsid transport toward the nucleus. Journal of Virology 80: 5494–98.

Arce R C F D, Almeid R S, Silva T C, Franco A C, Spilki F, Roehe P M and Arns C W. 2002. Restriction endonuclease and monoclonal antibody analysis of Brazilian isolates of bovine herpesviruses types 1 and 5. Veterinary Microbiology 88: 315–24.

Benoît M, Julien T, Philippe K, Frédéric S and Etienne T. 2007. Bovine herpes virus 1 infection and infectious bovine rhinotracheitis. Veterinary Research 38: 181–209.

Boelaert F P, Biront B, Soumare M, Dispas E, Vanopdenbosch J P, Vermeersch A, Raskin J, Dufey D, Berkvens P and Kerkhofs. 2000. Prevalence of Bovine herpesvirus-1 in the Belgian cattle population. Preventive Veterinary Medicine 45: 285–95.

Castrucci G, Martin W B, Frigeri F, Ferrari M, Salvatori D and Tagliati S.1997. A serological survey of Bovine herpesvirus-1 infection in selected dairy herds in northern and central Italy. Comparative Microbiology Immunology and Infectious Diseases 20: 315–17.

Chandranaik B M, Chethana C S and Renukaprasad C. 2010. Isolation of bovine herpes virus from semen and application of Real time PCR for diagnosis of IBR/IPV in clinical samples. Veterinary Arhive 80(4): 467–75.

Chintu Ravishankar, Nandi S, Chander V and Mohapatra T K. 2012. Glycoprotein C gene based molecular subtyping of bovine herpes virus isolate from Uttarpradesh, India. Indian Journal of Virology 23: 19–23.

Durham P J K and Hassard L E. 1990. Prevalence of antibodies to infectious bovine rhinotracheitis, parainfluenza-3, bovine respiratory syncytial and bovine viral diarrhoea viruses in cattle in Saskatchewan and Alberta. Canadian Veterinary Journal 31: 815–20.

Ganguly S and Mukhopadhay. 2010. Serological survey of infectious bovine Rhinotracheitis. Indian Veterinary Journal 87: 711–12.

Ghram A and Minocha H C. 1990. Neutralizing antibodies to Bovine herpesvirus-1 (BHV-1) and bovine parainfluenza-3 (PI-3) viruses in cattle in Tunisia. Arch Inst Pasteur Tunis 67: 25–31. Guarino H, Scarsi R, GIL A, Núñez A, Sienra R, Cerrnichiaro N and Piaggio J. 2000. Prevalence of bovine Herpesvirus-1,

Bovine Viral Diarrohea, Parainfluenza-3, and Bovine Respiratory Syncytial antibodies in dairy farms of Uruguay. Proceedings of the 21st Word Buiatrics Congress. Punta del Este, Uruguay, December.

Hage J J, Schukken Y H, Barkema H W, Benedictus G, Rijsewijk F A and Wentink G H. 1996. Population dynamics of bovine herpesvirus 1 infection in a dairy herd. Veterinary Microbiology 53: 169–80.

James N M and Edwards J D. 2011. Fenner’s Veterinary Virology. 4th edn. Academic Press. London, Boston, New York, Sydney, Tokyo, Toronto.

Kargar M S, Akhavizadegan M A, Charkhkar S and Meshkot M. 2001. Seroepidemological surveyfor antibodies against infectious bovine rhinotracheitis and bovine herpes 4 viruses among cattle in different provinces of Iran. Arch Razi Institute 52: 3–100.

Laszlo E, Gergely S, Janos T, Istvan M and Otto S. 2011. Symptomless intra-uterine transmission of bovine herpes virus 4 to bovine fetuses. Microbial Pathogenesis 24: 64–67.

Lopasev V N, Cartes M A, Monken C E, Velpandi A and Srinivasan A. 1991. An efficient and simple method of DNA extraction from whole blood and cell lines to identify infectious agents. Journal of Virological Methods 34: 105 –12.

Lowen K G and Darcel C L Q. 1985. A comparison of two methods for isolation of bovine herpes virus-1 from extended bovine semen. Theriogenology 23: 935–39.

Nandi S, Manish M and Manoj K. 2010. Serosurveillance of infectious bovine rhinotracheitis in buffalo bulls. Indian Veterinary Journal 87: 544–45.

Nuotio L, Neuvonen E and Hyyatiainen M. 2007. Epidemiology and eradication of IBR/IPV virus in Finland. Acta Veternarina Scandinavica 49: 1–6.

Patil S S, Desai G S, Shome R, Koppad K K, Gajendraghad M R and Prabhudas K. 2006. Cloning and sequencing if partial gB gene of Indian isolate of BoHV-1. Indian Journal of Comparative Microbiology Immunology and Infectious Diseases 27: 101–03.

Patil S S, Hemadri D, Sreekala K, Veeresh H, Chandranaik B M and Prabhudas K N. 2012. Genetic charecterisation of Bovine herpes virus isolates in India. Indian Journal of Animal Sciences 82 (8): 848–50.

Ranganatha S, Patil S S, Rathnamma D, Chandranaik B M and Isloor S. 2013. Molecular characterisation of IBR virus. Indian Journal of Animal Research (in press).

Reed L V and Muench H. 1938. A simple method of estimating fifty per cent end points. American Journal of Hygiene 27: 493– 97.

Richard A G, Thomas J K and Barbara A O. 2007. Kuby Immunology. 6th edn. W H Freeman and company, New York. Ros C and Belak S. 1999. Studies of genetic relationships between bovine, caprine, cervine, and rangiferine alpha herpesviruses and improved molecular methods for virus detection and identification. Journal of Clinical Microbiology 37: 1247–53. Rudi Weilben, Luiz C K, Teresinah F C and Marlon C R. 1992. Isolation of bovine herpes virus-1 from preputial swabs and semen of bulls with balanoposthitis. Journal of Veterinary

Diagnostic Investigation 4: 341–43.

Sambrook J, Fritsch E F and Maniatis T. 1989. Molecular Cloning. 2nd edn. Cold spring harbor lab press, USA.

Singh B K, Sreenivasan M A, Tongaonkar S S, Kant R and Choudhury P N R. 1986. Isolation of infectious bovine rhinotracheitis virus from semen and aborted materials of dairy cattle. Indian Journal of Animal Sciences 56 (8): 823–26.

Surendra K S L, Samir K R, Samayam P, Pokala J V, Velturi M, Chandrasekhar R, Mohana S and Villuppanoor A S. 2011. Genetic characterization of Indian isolates of BHV-1 by restriction endonuclease analysis and nucleotide sequencing of US 1.67 and UL 44 regions of viral genome. Proceedings of 25th International Conference of Indian Association of Veterinary Microbiologists, Immunologists and Specialists in Infectious Diseases. Pp. 14–15. Bangalore, India.

Tamura K, Dudley J, Nei M and Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24: 1596–99.

Van engelenburg F A C, Maes R K, Van Oirschot J T and Rijsewijk F A M. 1993. Development of a rapid and sensitive PCR for detection of herpes virus type-1 in bovine semen. Journal of Clinical Microbiology 31: 3129–35.

Wang J, O’Keefe J, Orr D, Loth L, Banks M, Wakeley P, West D, Card R, Ibata G, Van Maanen K, Thoren P, Isaksson M and Kerkhofs P. 2008. An international inter-laboratory ring trial to evaluate a real-time PCR assay for the detection of bovine herpesvirus 1 in extended bovine semen. Veterinary Microbiology 126: 11–19.