Determination of bovine rotavirus genotypes (G and P) circulating in Uttarakhand (2010-2012) by polymerase chain reaction
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Keywords:
Bovine G and P genotypes, Epidemiology, Rotavirus, RT-PCR, UttarakhandAbstract
The study describes the prevailing bovine rotavirus (RV) genotypes in high altitude areas of Uttarakhand state of India. The results confirmed the dominance of G3 genotype, which is responsible for diarrhea either alone or together with G8 and G10 genotypes. The P typing results on the other hand showed prevalence of only P[11] genotype in all bovine rotavirus isolates, confirming that least diversity exists in the major outer capsid protein gene (VP4) of bovines. The rotavirus existed in 4 types of genotypic combinations i.e. G3P[11], G10P[11], G3G8P[11] and G3G10P[11] in cattle calves of the region.
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References
Basera S S, Singh R, Vaid N, Sharma K, Chakravarti S and Malik Y P S. 2010. Detection of rotavirus infection in bovine calves by RNA-PAGE and RT-PCR. Indian Journal of Virology 21(2): 144–47.
Beg S A, Wani S A, Hussain I and Bhat M A. 2010. Determination of G and P type diversity of group A rotaviruses in faecal samples of diarrhoeic calves in Kashmir, India. Letters in Applied Microbiology 51: 595–99.
Bhat M A, Wani S A, Taku A and Dutta T K. 2005. Molecular epidemiology of rotavirus infection in animals in India: An update. Indian Journal of Animal Sciences 75: 1276–82.
Dash S K, Tewari A, Kumar K, Goel A and Bhatia A K. 2011. Detection of Rotavirus from diarrhoeic cow calves in Mathura, India. Veterinary World 4: 554–56.
Estes M, Kapikian A. 2007. Rotaviruses. (Eds.) Knipe D M, Howley P M, Griffin D E, Lamb R A, Martin M A, Roizman B and Straus S E. Fields virology. pp. 1917–74. Philadelphia: Kluwer Health/Lippincott, Williams and Wilkins.
Ghosh S, Samajdar S, Sinha M, Kobayashi N, Taniguchi K and Naik T N. 2008. Molecular characterization of rare bovine group A rotavirus G15P[11] and G15P[21] strains from eastern India: identification of simian SA11–like VP6 genes in G15P[21] strains. Virus Genes 37: 241–49.
Ghosh S, Varghese V, Samajdar S, Sinha M, Kobayashi N and Naik T N. 2007. Molecular characterization of bovine group A rotavirus G3P[3] strains. Archives of Virology 152: 1935–40.
Gulati B R, Nakagomi O, Koshimura Y, Nakagomi T and Pandey R. 1999. Relative frequencies of G and P types among rotaviruses from Indian diarrheic cow and buffalo calves. Journal of Clinical Microbiology 37: 2074–76.
Kapikian A Z, Hoshino Y and Chanock R M. Rotaviruses. 2001. Fields Virology. 4th edn. pp. 1787–1833. (Eds) D M Knipe and P M Howley. Philadelphia, PA: Lippincott Williams and Wilkins.
Kusumakar A L, Savita, Malik Y P S, Minakshi and Prasad G. 2010. Genomic diversity among group A rotaviruses from diarrheic children, piglets, buffalo and cow calves of Madhya Pradesh. Indian Journal of Microbiology 50: 83–88.
Malik Y P S, Kumar N, Sharma K, Haq A A, Kumar A and Prasad M. 2013. Sequence and phylogenetic analysis of bovine rotavirus isolates (G6 genotypes) from India. Advances in Veterinary and Animal Sciences 1(1): 41–43.
Malik Y P S, Sharma K, Vaid N, Chakravarti S, Chandrashekar K M, Basera S S, Singh R, Minakshi, Prasad S, Gulati B R, Bhilegaonkar K N and Pandey A B. 2012. Frequency of group A rotavirus with mixed G and P genotypes in bovines: predominance of G3 genotype and its emergence in combination with G8/G10 types. Journal of Veterinary Science 13(3): 271– 78.
Manuja B K, Prasad M, Manuja A, Gulati B R and Prasad G. 2008. A novel genomic constellation (G10P[3]) of group A rotavirus detected from buffalo calves in northern India. Virus Research 138: 36–42.
Martella V, Banyai K, Matthijnssens J, Buonavoglia C and Ciarlet M. 2009. Zoonotic aspects of rotaviruses. Veterinary Microbiology 140: 246–55.
Matsuda Y and Nakagomi O. 1989. Antigenic and molecular characterization of bovine rotaviruses isolated in Japan. Research in Virology 140: 337–50.
Matthijnssens J, Ciarlet M, McDonald S M, Attoui H, Banyai K, Brister J R, Buesa J, Esona M D, Estes M K, Gentsh J R, Iturriza- Gomara M, Johne R, Kirkwood C D, Martella V, Mertens P P C, Nakagomi O, Parreno V, Rahman M, Ruggeri F M, Saif L J, Santos N, Steyer A, Taniguchi K, Patton J T, Desselberger U and Van Ranst M. 2011. Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG). Archives of Virology 156(8): 1397–13.
Matthijnssens J, Potgieter C A, Ciarlet M, Parreno V, Martella V, Banyai K, Garaicoechea L, Palombo E A, Novo V, Zeller M, Arista S, Gerna G, Rahman M and Ranst M V. 2009. Are human P[14] rotavirus strains the result of interspecies transmissions from sheep or other ungulates belonging to the mammalian order of Artiodactyla? Journal of Virology 83: 2917–29.
McDonald S M, Matthijnssens J, McAllen J K, Hine E, Overton L, Wang S, Lemey P, Zeller M, Van Ranst M, Spiro D J and Patton J T. 2009. Evolutionary dynamics of human rotaviruses: balancing reassortment with preferred genome constellations. PLoS Pathogens 5(10): e1000634.
Minakshi, Prasad G and Grover Y P. 2009. Occurrence of dual infection of bovine group A rotavirus in diarrhoeic calf in Haryana, India. Indian Journal of Animal Sciences 79(12): 1205–08.
Minakshi, Prasad G, Malik Y P S and Pandey R. 2005. G and P genotyping of bovine group A rotaviruses in faecal samples of diarrhoeic calves by DIG-labelled probes. Indian Journal of Biotechnology 4: 93–99.
Nakagomi O and Nakagomi T. 1991. Genetic diversity and similarity among mammalian rotaviruses in relation to interspecies transmission of rotavirus. Archives of Virology 120: 43–55.
Nataraju S M, Chattopadhyay U K and Krishnan T. 2009. A study on the possibility of zoonotic infection in rotaviral diarrhoea among calves and buffalo calves in and around Kolkata, India.
European Review for Medical and Pharmacological Sciences 13: 7–11.
Niture G S, Karpe A G and Prasad M. 2011. Characterization of buffalo, poultry and human rotaviruses in characterization of buffalo, poultry and human rotaviruses in Western India. Veterinarski Arhiv 81: 307–19.
Parashar U D, Gibson C J, Bresee J S and Glass R I. 2006. Rotavirus and severe childhood diarrhea. Emerging Infectious Diseases 12: 304–06.
Rai R B, Hansha A, Rai S, Singh B, Kumar H, Singh A K, Damodaran T and Dhama K. 2011. Prevalence of rota and coronavirus infections in calves of Barabanki and Raebareli districts of Uttar Pradesh. Indian Journal of Veterinary Pathology 35: 73–74.
Saravanan M, Parthiban M and Ramadass P. 2006. Genotyping of rotavirus of neonatal calves by nested-multiplex PCR in India. Veterinarski Arhiv 76: 497–05.
Sharma G, Anil T, Rajesh C and Bhat M A. 2009. Prevalence of bovine rotaviral diarrhoea in Jammu. Indian Journal of Virology 20: 7–12.
Singh T C and Jhala M K. 2011. G-typing of bovine rotaviruses by using VP7 gene specific heminested RT-PCR from diarrhoeic calf faecal samples. Buffalo Bulletin 30: 113–38.
Varshney B, Jagannath M R, Vethanayagam R R, Kodhandharaman S, Jagannath H V, Gowda K, Singh D K and Rao C D. 2002. Prevalence of, and antigenic variation in, serotype G10 rotaviruses and detection of serotype G3 strains in diarrheic calves: implications for the origin of G10P11 or P11 type reassortant asymptomatic strains in newborn children in India. Archives of Virology 147: 143–65.
Wani S A, Bhat M A, Ishaq S M and Ashrafi M A. 2004. Determination of bovine rotavirus G genotypes in Kashmir, India. Revue Scientifique et Technique Office International des Eµpizooties 23(3): 931–36.
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