Detection of bovine viral diarrhoea virus (BVDV) in peripheral blood mononuclear cells (PBMCs) of BVDV antibody positive Indian cattle following mitogen stimulation

V GUPTA; N MISHRA; K RAJUKUMAR; S P BEHERA; P DUBEY

doi:10.56093/ijans.v84i2.37833

Authors

V GUPTA Indian Veterinary Research Institute, Anand Nagar, Bhopal, Madhya Pradesh 462 021 India
N MISHRA Indian Veterinary Research Institute, Anand Nagar, Bhopal, Madhya Pradesh 462 021 India
K RAJUKUMAR Indian Veterinary Research Institute, Anand Nagar, Bhopal, Madhya Pradesh 462 021 India
S P BEHERA Indian Veterinary Research Institute, Anand Nagar, Bhopal, Madhya Pradesh 462 021 India
P DUBEY Indian Veterinary Research Institute, Anand Nagar, Bhopal, Madhya Pradesh 462 021 India

https://doi.org/10.56093/ijans.v84i2.37833

Keywords:

Bovine viral diarrhea virus, BVDV antibody, Cattle, Peripheral blood mononuclear cells, PHA

Abstract

The aim of the present study was possible detection of BVDV in peripheral blood mononuclear cells (PBMCs) of antibody positive naturally infected Indian cattle following phytohaemagglutinin A (PHA) stimulation. Of 250 serum samples from cattle in organized dairy firms in India tested by virus neutralization test (VNT), 93 cattle (37.2%) were found positive for BVDV neutralizing antibodies. The PBMCs from BVDV-immune cattle were stimulated with 5µg/ml of PHA for 72 h prior to BVDV RNA detection by RT-PCR or infectious virus detection by virus isolation. Of the 93 BVDV immune field cattle tested positive, BVDV RNA was detected only in PHA stimulated PBMCs of 3 cattle, while no infectious BVDV could be detected by virus isolation. This is for the first time, that BVDV RNA was detected following mitogen (PHA) stimulation of PBMCs in BVDV antibody positive Indian cattle.

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