Omp31 gene based molecular detection of Brucella melitensis from serum samples of goats

A SINGH; V K GUPTA; A KUMAR; V K SINGH; SHIVASARANAPPA N

doi:10.56093/ijans.v84i3.38697

Authors

A SINGH Pandit Deen Dayal Upadhayay Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh 281 001 India
V K GUPTA Pandit Deen Dayal Upadhayay Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh 281 001 India
A KUMAR Pandit Deen Dayal Upadhayay Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh 281 001 India
V K SINGH Pandit Deen Dayal Upadhayay Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh 281 001 India
SHIVASARANAPPA N Central Institute for Research on Goats (CIRG), Makhdoom 281 001

https://doi.org/10.56093/ijans.v84i3.38697

Keywords:

Brucellosis, Goat, Omp31gene, Serum PCR

Abstract

The present study analyses the sensitivity and specificity of OMP31 gene based amplification directly from sera samples. Sero-positive animals (73) in rOmp31 i-ELISA were subjected to Omp31 gene amplification from sera samples and only 40 samples amplified 723kb product suggesting these as true positive animals. When similar test was applied with the sera samples of 16 infected (culture positive) and 20 non-infected (culture negative) goats, all the culture positive animals revealed 723 kb product while none of the culture negative goats revealed amplification. Hence serum based PCR appeared to be 100% sensitive and specific in truly infected animals. Thus serum is the optimal specimen for the diagnosis of brucellosis and leads to simplification of assay and shortens turnaround time.

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