Phenotypic and antigenic characterization of Mycoplasma gallisepticum by RFLP and SDS-PAGE

VADIVALAGAN K; SUKUMAR K; SUDHA RANI V; SIVACHANDRAN M; DORAIRAJAN N; MANICAVASAKA DINAKARAN A

doi:10.56093/ijans.v84i3.38708

Authors

VADIVALAGAN K Veterinary College and Research Institute, Namakkal, Tamil Nadu 637 002 India
SUKUMAR K Veterinary College and Research Institute, Namakkal, Tamil Nadu 637 002 India
SUDHA RANI V SRRDL, IAH&VB, Bengaluru, Karnataka 560 024
SIVACHANDRAN M Madras Veterinary College, Chennai, Tamil Nadu 600 007
DORAIRAJAN N Veterinary College and Research Institute, Namakkal, Tamil Nadu 637 002 India
MANICAVASAKA DINAKARAN A Veterinary College and Research Institute, Namakkal, Tamil Nadu 637 002 India

https://doi.org/10.56093/ijans.v84i3.38708

Keywords:

Mycoplasma gallisepticum, PCR, Phenotypic variation, RFLP, SDS-PAGE

Abstract

Phenotypic and antigenic variations among Mycoplasma gallisepticum vaccine 6/85 and field isolates were investigated by SDS-PAGE, RFLP and haemagglutination inhibition test. The SDS-PAGE system followed by photographic analysis showed weak variability between isolates, being the major difference close to the 150 kDa level where a prominent peptide band was detected only in the MG4 isolate. After digestion with Eco R1, two bands of sizes approximately 330 bp and 190 bp could be appreciated only in MG4 field isolate. Hyperimmune serum was used in serological test for this antigenic variability study. There were strong cross reaction between isolates. These studies verified the utility of newer technologies for disease outbreak investigation and the possibility of MG causing disease in layers under the field condition.

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