Role of antioxidant additives in the protection of DNA integrity of buck spermatozoa with RAPD assay

SONIA SARASWAT; S K JINDAL; S D KHARCHE; P K ROUT; R RANJAN; R PRIYADHARSINI

doi:10.56093/ijans.v84i3.38713

Authors

SONIA SARASWAT Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
S K JINDAL Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
S D KHARCHE Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
P K ROUT Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
R RANJAN Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
R PRIYADHARSINI Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India

https://doi.org/10.56093/ijans.v84i3.38713

Keywords:

Antioxidants, Buck semen, Random amplified polymorphic DNA

Abstract

The role of antioxidants (reduced glutathione, ascorbic acid and α-tocopherol) on DNA integrity with the random amplified polymorphic DNA (RAPD) was studied. This assay relies on presence or absence of amplification product on ethidium bromide stained agarose gels. The size of amplification products ranged from 190 to 1,900 base pair. Antioxidants effectively protect sperm DNA from damage. Moreover, random amplified polymorphic DNA technique can be used for the studies of the molecular seminal characteristics of buck semen.

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