Changes in sera proteome in relation to day of pregnancy in early pregnant buffaloes

A K BALHARA; MEENAKSHI GUPTA; A K MOHANTY; S K PHULIA; R K SHARMA; SURENDER SINGH; INDERJEET SINGH

doi:10.56093/ijans.v84i4.39841

Authors

A K BALHARA Central Institute for Research on Buffaloes, Hisar, Haryana 125001 India
MEENAKSHI GUPTA Central Institute for Research on Buffaloes, Hisar, Haryana 125001 India
A K MOHANTY Central Institute for Research on Buffaloes, Hisar, Haryana 125001 India
S K PHULIA Central Institute for Research on Buffaloes, Hisar, Haryana 125001 India
R K SHARMA Central Institute for Research on Buffaloes, Hisar, Haryana 125001 India
SURENDER SINGH National Dairy Research Institute, Karnal
INDERJEET SINGH Central Institute for Research on Buffaloes, Hisar, Haryana 125001 India

https://doi.org/10.56093/ijans.v84i4.39841

Keywords:

Buffaloes, Early pregnancy, Proteomics, Serum

Abstract

The present study was planned for studying the sequential changes in early pregnant buffalo for the development of diagnostics for early pregnancy. In the present study, sera samples obtained at weekly intervals from early pregnant (day 0 to day 42 post-AI) buffaloes, and on days 0, 7 and 14 from non-pregnant cyclic buffaloes, were subjected to depletion of high abundant proteins followed by 2-dimensional gel electrophoresis and densitometric analysis. At least 65 2-D gel spots exhibited up-regulation, down-regulation or specific appearance at a specific stage during early buffalo pregnancy, except for the spots correlating with the high abundant proteins’ location. Comparison with ExPASy and NCBI databases matched 48 of these spots with known proteins, but with varying degrees of confidence in terms of Mascot score and the species. Although high abundant proteins were depleted before 2-D electrophoresis, yet in some of the picked spots isoforms of common abundant proteins, viz. serum albumin, igg, serrotransferrin, complement and mhc molecules, were found. synaptojanin-1, apolipoprotein a-1, apolipoprotein b, keratin 10 and von Willebrand factors were some of the proteins identified in these spots, which have a documented role in embryogenesis and early pregnancy.

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References

Agarwal A, Gupta S and Sikka S. 2006. The role of free radicals and antioxidants in reproduction. Current Opinion in Obstetrics and Gynecology 18: 325–32.

Atsunobu T, Hamano S, Yamanaka A, Hanada T, Ishibashi T, Mak T W, Yoshimura A and Yoshida H. 2003. Cutting edge: role of IL-27/WSX-1 signalling for induction of T-bet through activation of STAT1 during initial Th1 commitment. Journal of Immunology 170 (10): 4886–90

Authie E and Pobel T. 1990. Serum haemolytic complement activity and C3 levels in bovine trypanosomosis under natural conditions of challenge—early indications of individual susceptibility to disease. Veterinary Parasitology 35 (1–2): 43–59.

Ayalon N. 1978. A review of embryonic mortality in cattle. Journal of Reproduction and Fertility 54(2): 483–93.

Bainbridge D R J, Sargent I L and Ellis S A. 2001. Increased expression of major histocompatibility complex (MHC) class I transplantation antigens in bovine trophoblast cells before fusion with maternal cells. Reproduction 122: 907 – 13.

Beindorff N, Nagai K, Shirasuna K, Herzog K, Hoeffmannn K, Sasaki M, Bollwein H and Miyamoto A. 2010. Vascular changes in the corpus luteum during early pregnancy in cow. Journal of Reproduction and Development 56: 263–270.

Committee on Reproductive Nomenclature. 1972. Recommendations for standardizing bovine reproductive terms. Cornell Veterinarian 62 (2): 216–37.

Finucane K A, McFadden T B, Bond J P, Kennelly J J and Zhao F Q. 2008. Onset of lactation in the bovine mammary gland: gene expression profiling indicates a strong inhibition of gene expression in cell proliferation. Functional and Integrative Genomics 8 (3): 251–64.

Hafez E S E. 1993. Reproduction in Farm Animals. 6th edn, pp 188–201. Lea and Febiger, Philadelphia, USA.

Hayes J D and Strange R C. 1995. Potential contribution of the glutathione S-transferase supergene family to resistance to oxidative stress. Free Radical Research 22(3): 193–207.

Herrera F, Chen Q, Fischer W H, Maher P and Schubert D R. 2009. Synaptojanin-1 plays a key role in astrogliogenesis: possible relevance for Down’s syndrome. Cell Death and Differentiation 16: 910–20.

Kim H R, Han R X, Yoon J T, Park C S and Jin D I. 2010. A two- dimensional electrophoresis reference map for the bovine placenta during late pregnancy. Proteomics 10: 564–73.

Meirelles F V, Caetano A R, Watanabe Y F, Ripamonte P, Carambula S F, Merighe G K and Garcia S M. 2004. Genome activation and developmental block in bovine embryos. Animal Reproduction Science 82–83: 13–20.

Morris D and Diskin M. 2008. Effects of progesterone on embryo survival. Animal 2: 1112–19.

Niswender G D, Juengel J L, Silva P J, Rollyson M K and McIntush E W. 2000. Mechanisms controlling the function and life span of the corpus luteum. Physiological Reviews 80:1–29.

Riding G A, Jones A, Holland M K, Hill J R and Lehnert S A. 2008. Proteomic analysis of bovine conceptus fluids during early pregnancy. Proteomics 8:160–77.

Roy A C, Loke D F, Saha N, Viegas O A, Tay J S and Ratnam S S. 1994. Interrelationships of serum paraoxonase, serum lipids and apolipoproteins in normal pregnancy. A longitudinal study. Gynaecology and Obstetrics Investigations 38 (1): 10–13.

Telford N A, Watson A J, and Schultz G A. 1990. Transition from maternal to embryonic control in early mammalian development: A comparison of several species. Molecular Reproduction and Development 26: 90–100.

Uchimura A, Hidaka Y, Hirabayashi T, Hirabayashi M and Yagi T. 2009. DNA polymerase d is required for early mammalian embryogenesis. PLoS ONE 4 (1) e4184. (online journal available at www.plosone.org)