Dose dependent effect of pregnant mare serum gonadotropin and human chorionic gonadotropin on in vitro maturation of goat oocytes

J KOUAMO; S D KHARCHE

doi:10.56093/ijans.v84i4.39842

Authors

J KOUAMO University of Ngaoundere, PO BOX: 454. Ngaoundere-Cameroon
S D KHARCHE Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India

https://doi.org/10.56093/ijans.v84i4.39842

Keywords:

Goats, hCG, In vitro maturation, Oocytes, PMSG

Abstract

This study was aimed to evaluate the dose dependent effect of pregnant mare serum gonadotropin and human chorionic gonadotropin on in vitro maturation goat oocytes. The study was planned in 2 experiments. In the first experiment effect of pregnant mare serum gonadotropin supplementation on nuclear maturation of goat oocytes using 5 different concentrations of PMSG, was studied. The treatment was divided into group 1 (control medium containing TCM-199, sodium pyruvate (0.25mM), gentamycin (50µg/ml), L-glutamine (100µg/ml), estradiol 17-ß (1µg/ml), BSA (3mg/ml), supplemented with 10% FBS); group 2 (control medium + 10 IU/ml PMSG); group 3 (control medium + 20 IU/ml PMSG); group 4 (control medium + 30 IU/ml PMSG); group 5 (control medium + 40 IU/ml PMSG); and group 6 (control medium + 50 IU/ml PMSG). The oocytes of these treatment groups were matured in maturation media for 27 h in humidified atmosphere of 5% CO₂ at 38.5°C in a CO₂ incubator. The maturation rates achieved in groups 1–6 were 20.0, 48.51, 74.63, 74.15, 75.0 and 76.33%, respectively. In experiment 2, the effect of hCG supplementation on nuclear maturation of goat oocytes using 3 different concentrations of PMSG, was studied. Second experiment was divided into group 1 (control medium + 20 IU/ml PMSG +10IU/ml hCG), group 2 (control medium + 20 IU/ml PMSG +20IU/ml hCG) and group 3 (control medium + 20 IU/ml PMSG +30IU/ml hCG). The maturation rates achieved in groups 1–3 were 77.0, 85.19 and 84.22%, respectively. It can be concluded that supplementation of 20 IU/mL PMSG and 20IU hCG in maturation media significantly increased the maturation rate.

Downloads

Download data is not yet available.

References

Brackett B G and Zuelke K A. 1993. Analysis of factors involved in the in-vitro production of bovine embryos. Theriogenology 39: 61–64.

Chauhan M S, Katiyar P K, Madan M L, Singla S K and Manik M S. 1996. Influence of follicle stimulating hormone on in vitro maturation and cleavage of buffalo (Bubalus bubalis) oocytes after in vitro fertilization. Theriogenology 45: 243.

Cognie Y, Baril G, Poulin N and Mermillod P. 2003. Current status of embryo technologies in sheep and goat. Theriogenology 59: 171–88.

Farag I M, Girgis S M, Khalil W K B, Hassan N H A, Sakr A A M, Abd Allah S M and Ali N I. 2009. Effect of hormones, culture media and oocyte quality on in vitro maturation of Egyptian sheep oocytes. Journal of Applied Bioscience 24: 1520–34.

Feng M S, Feng M M S, Liang M Y, Ziyu L G and Le C Z. 2002. Effect of sera and gonadotrophin on in vitro nuclear maturation of goat oocytes. Chinese Journal of Veterinary Science 22: 334–36.

Freitas V J F, Serova I A, Andriiva L I, Dvoriantchikov G, Lopes Junior E S, Teixeira D I A, Moura R R, Melo L M, Pereira A F, Carvalho A C C and Serov O. 2007. Production of transgenic goat (Capra hircus) with human granulocyte colony stimulating factor (hG-CSF) gene in Brazil. Anais da Academia Brasileira de Ciências 79 : 585–592.

Gupta P S, Nandi S, Ravindranatha B M and Sarma P V. 2001. Effect of commercially available PMSG on maturation, fertilization and embryo development of buffalo oocytes in vitro. Reproduction Fertility and Development 13 (5–6): 355–60.

Jimenez-Macedo A R, Paramio M T, Anguita B, Morato R and Romaguera. 2007. Effect of ICSI and embryo biopsy on embryo development and apoptosis according to oocyte diameter in prepubertal goat oocytes. Theriogenology 67: 1399–1408.

Keskintepe L, Darwish G M, Kenimer A T and Brackett B G. 1994. Term development of caprine embryos derived from immature oocytes in vitro. Theriogenology 42: 527–35.

Kharche S D, Goel A K, Jindal S K , Sinha N K and Yadav P. 2008. Effect of somatic cells co-culture on cleavage and development of in vitro fertilized embryos. Indian Journal of Animal Sciences 78; 686–92.

Kharche S D, Goel A K, Jindal S K and Sinha N K. 2006. In vitro maturation of caprine oocytes in different concentrations of estrous goat serum. Small Ruminant Research 64, 186–89.

Kharche S D, Goel P, Jha B K, Goel A K and Jindal S K. 2011. Factors influencing in-vitro embryo production efficiency of caprine oocytes: A review. Indian Journal of Animal Sciences 81 (4), 344–61.

Ledda S, Bogliole L, Calvia P, Leom G and Naitana S. 1997. Meiotic progression and developmental competence of oocytes collected from juvenile and adult ewes. Journal of Reproduction and Fertility 109: 73–78.

Martino A, Palomo M J, Mogas T and Paramio M T. 1994. Influence of the collection technique of prepubertal goat oocytes on in vitro maturation and fertilization. Theriogenology 42: 859–73.

Mogas T, Palomo M J, Izquierdo M D and Paramio M T. 1997a. Developmental capacity of in vitro matured and fertilized oocytes from prepubertal and adult goats. Theriogenology 47: 1189–1203.

Mogas T, Palomo M J, Izquierdo M D and Paramio M T. 1997b. Morphological events during in-vitro fertilization of prepubertal goat oocytes matured in-vitro. Theriogenology 48: 815–29.

Ongeri E M, Bormann C L, Butler R E, Melican D, Gavin W G and Echelard Y. 2001. Development of goat embryos after in vitro fertilization and parthenogenetic activation by different methods. Theriogenology 55: 1933–45.

Pawshe C H, Palanisamy A, Taneja M, Jain S K and Totey S M. 1996. Comparison of various maturation treatments on in vitro maturation of goat oocytes and their early embryonic development and cell numbers. Theriogenology 46: 971–82.

Rahman A N M A, Abdullah R B and Wan-Khadijah W E. 2008. In-vitro maturation of oocytes with special reference to goat: a review. Biotechnology 7: 599–611.

Seydou S, Oladele G, Eugene A and Seyoum G. 1999. The effects of serum source and hormone supplementation on goat oocytes maturation, fertilization and early embryo development during the non-breeding season. Society for the Study of Reproduction, 32nd Annual Meeting, July 31–August 3.

Sirard M A, Defroster S and Aside M. 2007. In vivo and in vitro effects of FSH on oocyte maturation and developmental competence. Theriogenology 68: 71–76.

Snedecor G W and Cochran W G. 1989. Statistical Methods. 8th edn, pp.125–128. Iowa State University Press, Ames, Iowa, USA.

Yadav E N, Kharche S D, Goel A K, Jindal S K and Johri D K. 2007. Comparative efficacy of different techniques for oocytes recovery from pre pubertal goat ovaries. Indian Journal of Animal Sciences 77 (10): 988–990.

Yadav P, Kharche S D, Goel A K, Jindal S K and Sharma M C. 2010. Effect of hormones, EGF and ß-mercaptoethanol on in- vitro maturation of caprine oocytes. Reproduction Fertility and Development 22 (1): 337 (Abstract).