Electrophoretic profile of serum proteins from Dromedary camels

PREETI CHOUDHARY; DINESH CHANDRA

doi:10.56093/ijans.v84i4.39843

Authors

PREETI CHOUDHARY National Research Centre for Camel, Bikaner, Rajasthan 334 001 India
DINESH CHANDRA Maharana Pratap University of Agriculture and Technology, Udaipur, Rajasthan

https://doi.org/10.56093/ijans.v84i4.39843

Keywords:

Camel, Electrophoretic pattern, Immunoglobulin SDS-PAGE, Serum protein

Abstract

Electrophoretic pattern of serum protein and dialysed serum protein profiles were studied from apparently healthy camels on 10 and 15% SDS-PAGE in addition to total protein and immunoglobulin content. The electrophoretic pattern of camel serum protein revealed 7 prominent bands of molecular weights ranging from 28.21 kDa to 123.07 kDa on 10% SDS-PAGE and dialysed serum protein revealed 4 prominent bands of molecular weights ranging from 24.30 kDa to 110.87 kDa. The electrophoretic pattern of camel serum protein on 15% SDS PAGE revealed 8 prominent bands of molecular weights ranging from 0.005 to 121.04 kDa along with 5 bands of dialysed serum protein. There are about 7 lower molecular weight bands which may be of prealbumin and other lower molecular weights of proteins. The lower molecular weight bands may be of prealbumin. The band with higher molecular weight may be of different globulin fractions including immunoglobulins. Total protein and immunoglobulin content were low in present study suggesting that it is due to very hot temperature and limited grazing facility during summer.

Downloads

Download data is not yet available.

References

Al-Busadah K A. 2007. Some biochemical and haematological indices in different breeds of camels in Saudi Arabia. Scientific Journal of King Faisal University (Basic and Applied Sciences) 8(1): 131–42.

BgVV/GTZ. 1994. 11th Information from the service laboratory. Measurement of Neonatal gammaglobulins for Assessment of Colostral Immunoglobulin transfers. The Zinc Sulphate Turbidity Test BgVV, Berlin.

El-Bahrawy K A and El Hassanein E E. 2011. Seasonal variations of some blood and seminal plasma biochemical parameters of male dromedary camles. American-Eurasian Journal of Agriculture and Environment Science 10(3): 354–60.

Kataria N and Kataria A K. 2004. Blood profile during stress in dromedary camel. Veterinary Practitioner 5(2): 159–61.

Khadjeh G H. 1998. Electrophoretic pattern of serum proteins in the Iranian one humped camel (Caemlus dromedaries). Indian Journal of Animal Sciences 68 (5): 476–77.

Laemmli U K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriphage T4. Nature 227: 680–85.

Nazifi S, Saeb M and Snaashari H R. 2001. Using Polyacrylamide gel electrophoresis for determination of serum and synovial

fluid proteins in clinically healthy camels (Camelus dromedarius). Journal of Camel Practice and Research 8 (2): 80–84.

Patodkar V R, Somkumar A P, Parekar S and Khade N. 2010. Influence of sex on certain biochemical parameters in Nomadic Camels (Camelus dromedaries) nearby Pune, in Maharashtra. Veterinary World 3: 115–17.

Purohit S K, Jatkar P R and Ghosal A K. 1981. A note on the agar gel electrophoretic characterization of camel serum protein. Indian Veterinary Journal 58: 739–40.

Roy A and Sharma KN (1991). Immunoglobulin levels in camel serum and their antibacterial activity. Indian Journal of Comparative Microbiology and Immunology of Infectious Diseases 12(3):82–91.

Sena S D, Mal G and Dixit S K. 2011. Effect of herbal immune modulator on growth rate and protein profile of dromedary camel calves during summer stress. Journal of Camel Practice and Research 18 (1): 31–34.

Ungar-Waron H, Elias E, Gluckman A and Trainin Z. 1987. Dromedary IgG: purification, characterization and quantitation in sera of dams and new born. Israel Journal of. Veterinary Medicine 43: 198–203.

Weber K and Osborn M. 1969. The reliability of molecular weight determination of do-decyl sulphate polyacrylamide gel electophoresis. Journal of Biological Chemistry 244: 4406–12.