Changes in haematology, blood mineral profile, ultra structure and superoxide dismutase activities in erythrocytes in hypophosphetemic buffaloes given excess molybdenum

A K SHARMA; S N S RANDHAWA; S K UPPAL; R RANJAN

doi:10.56093/ijans.v84i5.40653

Authors

A K SHARMA Guru Angad Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
S N S RANDHAWA Guru Angad Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
S K UPPAL Guru Angad Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
R RANJAN Guru Angad Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India

https://doi.org/10.56093/ijans.v84i5.40653

Keywords:

Buffalo, Erythrocytes, Minerals, Molybdenosis, Phosphorus deficiency

Abstract

Phosphorus deficiency in buffaloes resulted into development of macrocytic, normochromic anaemia with significant decline in haemoglobin, packed cell volume and total erythrocyte count. Erythrocytic superoxide dismutase activity was markedly decreased in hypophosphataemic animals given excess molybdenum (T₂) in comparison to hypophosphataemic animals not given molybdenum (T₃). Scanning electron microscopy of erythrocytes in both T₂ and T₃ animals showed presence of spherocytes, discocytes and slight membrane corrugations; while erythrocytes in healthy control (T₁) appeared biconcave with mild poikilocytosis. From the present study it can be concluded that phosphorus deficiency induces ultrastructural abnormalities in erythrocyte, which is further aggravated by excess molybdenum supplementation. Increased oxidative damage may contribute structural changes in phosphorus deficiency and molybdenum toxicity.

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References

Akhtar M S, Lodhi L A, Ahmad I, Qureshi Z I and Muhammad G. 2012. Serum trace mineral variations in Nili- Ravi buffaloes suffering with prepartum vaginal prolapse in two different agro-ecological zones of Punjab, Pakistan. Theriogenology 77: 1328–33.

Benjamin M M. 1985. Outline of Veterinary Clinical Pathology. 3rd edn. Kalyani Publishers, Ludhiana, India.

Bhardwaj R M and Rana J P. 1988. ‘Surface electron microscopy of buffalo red blood cells.’ M V Sc Thesis. Haryana Agricultural University, Hisar, India. (fide II World Buffalo Congress, I.C.A.R., Pusa, New Delhi, India).

Clemens M R and Waller H D. 1987. Lipid peroxidation in erythrocytes. Chemistry and Physics of Lipids 45: 251–68.

Dimri U, Sharma M C, Swarup D, Ranjan R and Kataria M. 2008. Alterations in hepatic lipid peroxides and antioxidant profile in Indian water buffaloes suffering from sarcoptic mange. Research in Veterinary Science 85: 101–05.

Gomorri G. 1942. A modification of colorimetric phosphorus determination for the use with the photoelectric colorimeter. Journal of Laboratory and Clinical Medicine 27: 955–60.

Guyton A C. 1991. A Textbook of Medical Physiology. 8th edn. W.B. Saunders Company, West Washington Square, Philadelphia.

Hansen S L, Schlegel P, Legleiter L R, Lloyd K E and Spears J W. 2008. Bioavailability of copper from copper glycinate in steers fed high dietary sulfur and molybdenum. Journal of Animal Science 86: 173–79.

Hundal H S, Kumar R, Singh D and Manchanda J S. 2006. Available nutrient and heavy metal status of soils of Punjab, North-West India. Journal of Indian Society of Soil Science 54: 1–5.

Khan Z I, Ashraf M, Al-Qurainy F, Ahmad K, Gondal S and Fardous A. 2011. Studies on the transfer of copper from soil to pastures at different sampling periods: a case study of a semiarid region (Sargodha) in Pakistan. Biological Trace Element Research 141: 126–30.

Marklund S and Marklund G. 1974. Involvement of superoxide anion radical in the auto-oxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry 47: 469–74.

Ogawa E, Kobayashi K, Yoshiura N and Mukai J. 1989. Hemolytic anemia and red blood cell metabolic disorder attributable to low phosphorus intake in cows. American Journal of Veterinary Research 50: 388–92.

Özkan C, Kaya A, Aslan L and Akgül Y. 2012. Diagnosis and treatment of hypophosphatemia in young Turkeys. Pakistan Veterinary Journal 32: 125–27.

Radostits O M, Blood D C, Gay C C and Hinchcliff K W. 2000. Veterinary Medicine: A Textbook of Diseases of Cattle, Sheep, Pigs, Goats and Horses, 9th edn. W B Saunders, London.

Randhawa S S, Uppal S K, Joshi B P and Randhawa C S. 2009. Epidemiological and clinic-therapeutic studies on leucoderma in dairy animals. Veterinary Record 164: 631–32.

Raymond S and Wilkinson J H. 1963. Clinical Chemistry: Theory and Practice, Academic Press, New York, London.

Schalm O W, Jain N C and Carrol E J. 1975. Veterinary Haematology.3rd edn, pp. 54–70. Lea and Febiger, Philadelphia.

Snedecor G W and Cocharan W G. 1994. Stastical Methods. 9th edn. Affliated East-West Press Pvt. Ltd, Iowa State University Press, Iowa.

Underwood E J. 1981. The Mineral Nutrition of Livestock. 2nd edn. Commonwealth Agricultural Bureau. The Central Press, Aberdeen, U.K.

Xin X, Waterman D F, Hemben R W and Harmon R J. 1991. Effect of copper status on neutrophil function, superoxide dismutase and copper distribution in steers. Journal of Dairy Science 74: 3078–85.