Trend of association of BoLA-DQA1 alleles with FMDV vaccine elicited immune response in crossbred cattle

R K VANDRE; A K SHARMA; G R GOWANE; M SANKAR; A SANYAL; POONAM BISHT; B PATTNAIK

doi:10.56093/ijans.v84i6.41569

Authors

R K VANDRE College of Veterinary Sciences and Animal Husbandry, Rewa
A K SHARMA Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
G R GOWANE Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
M SANKAR Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
A SANYAL Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
POONAM BISHT Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
B PATTNAIK Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v84i6.41569

Keywords:

Bovine leukocyte antigen, Crossbred cattle, DQA1, FMD, Foot and mouth disease

Abstract

Major histocompatibility complex molecules play a major role in immunological defense against pathogens. Polymorphism of bovine leukocyte antigen (BoLA) DQA1 gene is being intensively investigated for potential association with economically important diseases of cattle. Accordingly, we investigated the association of DQA1 Exon 2 polymorphism as evidenced by the variation in the binding pockets with variability in immune response to inactivated trivalent (O, A and Asia1) foot and mouth disease virus (FMDV) vaccine in a closed population of crossbred cattle. Antibody titer of >1.8 was set as the cutoff value to distinguish the protected (> 1.8) and unprotected (<1.8) animals. Eleven different alleles of over 1% frequency were detected in the population. Allele *0102 occupied highest rank followed by *10011 and *1402 for protective immune response while the allele *1401 ranked lowest for unprotected immune response for all the 3 serotypes. The correlation coefficient (ρ) of overall rank with individual ranks of serotype O, A, and Asia1 was also high in magnitude and positive. The rank correlations were statistically significant for all the serotypes except between O and Asia. Logistic regression analysis revealed that the effect of DQA1 alleles was nonsignificant on vaccine elicited immune response based on Wald statistics. However, it is evident that odds of protection is high [Exp (β)>1] for a good proportion of DQA1 alleles in a given serotype. The knowledge has potential implications in future selection programmes if integrated with the complete BoLA haplotype details and production traits of the herd.

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