Economic impact of ovine footrot and serological diversity and virulence of Dichelobacter nodosus in north Kashmir, India

Z A KABLI; S A WANI; I HUSSAIN; M A BHAT; M A RATHER; S N MAGRAY

doi:10.56093/ijans.v84i7.42089

Authors

Z A KABLI Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shuhama (Alusteng), Srinagar 190 006 India
S A WANI Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shuhama (Alusteng), Srinagar 190 006 India
I HUSSAIN Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shuhama (Alusteng), Srinagar 190 006 India
M A BHAT Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shuhama (Alusteng), Srinagar 190 006 India
M A RATHER Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shuhama (Alusteng), Srinagar 190 006 India
S N MAGRAY Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shuhama (Alusteng), Srinagar 190 006 India

https://doi.org/10.56093/ijans.v84i7.42089

Keywords:

Dichelobacter nodosus, Footrot, PCR, Serogrouping, Sheep, Virulence

Abstract

The present study was aimed to determine the prevalence and economic impact of footrot as well as to determine the serological diversity and strain differentiation of Dichelobacter nodosus in north Kashmir, India, comprising district Baramulla, Bandipora and Kupwara. The overall prevalence was 14.77% with the estimated economic impact to the tune of Rs 41 million annually to the sheep production in north Kashmir. The prevalence was highest (17.41%) in district Kupwara and lowest (12.71%) in district Bandipora while district Baramulla recorded a prevalence rate of 14.44%. Out of 300 samples collected from footrot lesions of naturally infected sheep, 150 detected positive for D. nodosus by PCR. Over all prevalence of serogroups B, E and I of D. nodosus was 92.7, 32 and 26%, respectively. Infection due to single serogroup was found in 87 samples while, 63 samples revealed mixed infection of 2 or 3 serogroups. Out of 226 isolates of D. nodosus from positive samples, 139 (61.50%) belonged to serogroup B, 48 (21.23%) to E and 39 (17.25%) to I. Virulence characterization revealed that 66.80% showed the presence of integrase (intA) gene, thus were considered as virulent strains. Serogroup wise intA gene was found in 91 (65.46%) isolates of serogroup B, 33 (68.75%) of E and 27 (69.23%) of I. These findings suggested the need for incorporation of serogroups B and E in the formulation of a vaccine to effectively combat footrot in north Kashmir.

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References

Cheetham B F, Tanjung L R, Sutherland M, Druitt J, Green G, McFarlane J, Bailey G D, Seaman J T and Katz M E. 2006. Improved diagnosis of virulent footrot using intA gene. Veterinary Microbiology 116: 166–74. DOI: https://doi.org/10.1016/j.vetmic.2006.04.018

Claxton P D, RibeiroL A and Egerton J R. 1983. Classification of Bacteroides nodosus by agglutination test. Australian Veterinary Journal 70: 123–26.

Claxton P D. 1986. Serogrouping of Bacteroides nodosus isolates. Proceedings of a Workshop, Melbourne, 1985. pp. 131– 134 CSIRO, Division of Animal Health and Australian Wool Corporation, Glebe, Sydney, N.S.W, Australia.

Dhungyel O P, Whittington R J and Egerton J R. 2002. Serogroup specific single and multiplex PCR with pre-enrichment culture and immunomagnetic bead capture for identifying strains of D. nodosus in sheep with footrot prior to vaccination. Molecular and Cellular Probes 16: 285–96. DOI: https://doi.org/10.1006/mcpr.2002.0427

Farooq S, Wani S A, Hussain I and Bhat M A. 2010. Prevalence of ovine footrot in Kashmir, India and molecular characterization of Dichelobacter nodosus by PCR. Indian Journal of Animal Sciences 80: 826–30

Ghimire S C, Egerton J R, Dhungyel O P and Joshi H D. 1998.Identification and characterization of serogroup M among Nepalese isolates of Dichelobacter nodosus, the transmitting agent of footrot in small ruminants. Veterinary Microbiology 62: 217–33. DOI: https://doi.org/10.1016/S0378-1135(98)00206-5

Grogono T R and Johnston A M. 1997. A Study of Ovine Lameness. MAFF Open Contract OC 5945K, DEFRA Publications, London.

Gurung R B, Tshering P, Dhungyel O P and Egerton J R. 2006. Distribution and prevalence of footrot in Bhutan. Veterinary Journal 171: 346–51. DOI: https://doi.org/10.1016/j.tvjl.2004.11.012

Hindmarsh F and Fraser J. 1985. Serogrouping of Bacteroides nodosus isolated from ovine footrot in Britain. Veterinary Record 116: 187–88. DOI: https://doi.org/10.1136/vr.116.7.187

Katz M E, Howarthm P M, Young W K, Rifkin G G, Depiazi L J and Rood J I. 1991. Identification of three gene regions associated with virulence in Dichelobacter nodosus, the causative agent of ovine footrot. Journal of General Microbiology 137: 2117–24. DOI: https://doi.org/10.1099/00221287-137-9-2117

Kennan R M, Dhungyel O P, Whillington R J, Egerton J R and Roods J I. 2003. Transformation-mediated serogroup conversion of Dichelobacter nodosus. Veterinary Microbiology 92: 169–78. DOI: https://doi.org/10.1016/S0378-1135(02)00359-0

La Fontaine S, Egerton J R and Rood J I. 1993. Detection of Dichelobacter nodosus using species-specific oligonucleotides as PCR primers. Veterinary Microbiology 35: 101–17. DOI: https://doi.org/10.1016/0378-1135(93)90119-R

Marshall D J, Walker R J, Cullis B R and Luff M F. 1991. The effect of footrot on body weight and wool growth of sheep. Australian Veterinary Journal 68: 45–49. DOI: https://doi.org/10.1111/j.1751-0813.1991.tb03126.x

Nieuwhof GJ and Bishop SC. 2005. Costs of the major endemic diseases of sheep in Great Britian and the potential benefits of reduction in disease impacts. Animal Science 81: 57–67. DOI: https://doi.org/10.1079/ASC41010023

Palmer M A. 1993. A gelatin test to detect activity and stability of proteases produced by Dichelobacter (Bacteroides) nodosus. Veterinary Microbiology 36: 113–22. DOI: https://doi.org/10.1016/0378-1135(93)90133-R

Rather M A, Wani S A, Hussain I, Bhat M A, Kablia Z A and Magraya S N. 2011. Determination of prevalence and economic impact of ovine footrot in central Kashmir India with isolation and molecular characterization of Dichelobacter nodosus. Anaerobe 17: 73–77. DOI: https://doi.org/10.1016/j.anaerobe.2011.02.003

Stewart D. 1989. Footrot and foot abscesses of ruminants. Footrot in Sheep. pp. 5–45. (Eds) Egerton J R, Yong W K and Riffkin G G. Boca Raton. CRC Press, Florida.

Stewart D J. 1979., The role of elastase in differentiation of Bacteroides nodosus infections in sheep and cattle. Research in Veterinary Science 27: 99–105. DOI: https://doi.org/10.1016/S0034-5288(18)32866-2

Stewart D J, Kortt A A and Lilley G G.1990. New approaches to footrot vaccination and diagnosis utilising the proteases of Bacteroides nodosus. Advances in Veterinary Dermatology pp. 359–369. (Eds) Tscharner C von and Halliwell R E W. Bailliere Tindall.

Thomas J H. 1964. The pathogenesis of footrot in sheep with reference to protease of Fusiformis nodosus. Australian Journal of Agricultural Research 15: 1001–16. DOI: https://doi.org/10.1071/AR9641001

Thorley C M. 1976. A simplified method for the isolation of Bacteroides nodosus from ovine footrot and studies on its colony morphology and serology. Journal of Applied Bacteriology 40: 301–09. DOI: https://doi.org/10.1111/j.1365-2672.1976.tb04178.x

Wani S A, Samanta I and Shah S S. 2007. Isolation and characterization of Dichelobacter nodosus from ovine and caprine footrot in Kashmir, India. Research in Veterinary Science 83: 141–44. DOI: https://doi.org/10.1016/j.rvsc.2006.11.006

Wassink G J, Grogono-Thomas R, Moore L J and Green L E. 2003. Risk factors associated with the prevalence of footrot in sheep from 1999 to 2000. Veterinary Record 152: 351–58. DOI: https://doi.org/10.1136/vr.152.12.351