Pesticide residue accumulation in buffalo ovaries: a potential hazard to fertility

U RATNAKARAN; S P S GHUMAN; J S BEDI; J P S GILL

doi:10.56093/ijans.v84i7.42107

Authors

U RATNAKARAN Kerala Veterinary and Animal Sciences University, Kerala
S P S GHUMAN Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
J S BEDI Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
J P S GILL Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India

https://doi.org/10.56093/ijans.v84i7.42107

Keywords:

Blood, Buffalo, Follicular fluid, Ovary, Pesticide residue

Abstract

The present study was conducted on 58 female buffaloes subjected to slaughtering at a local abattoir. The study investigated the presence of pesticide residues in their blood, ovarian tissue as well as follicular fluid samples. These samples were subjected to gas chromatography (GC) to detect the presence of residues of organochlorine pesticides, organophosphorous pesticides and synthetic pyrethroids, followed by their confirmation using gas chromatography mass spectrometry (GCMS). About 32.7% blood, 53.4% ovarian tissue and 21.4% follicular fluid samples were found positive for pesticide residue(s) and their respective alarming levels of pesticide residues were 47.7±113.7 ng/ml, 124.3±106.1 ng/g and 245.6±477.1 ng/ml. The most detected pesticide residues in the ovarian/ follicular fluid samples were DDT / endosulphan and their metabolites. In conclusion, a much higher load of pesticide residues in ovarian/follicular fluid of buffaloes as comapred to their blood suggested the potential hazard of these residues to fertility status of buffaloes.

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References

Battu R, Singh P and Joia B. 1989. Contamination of bovine (buffalo, Bubalus bubalis (L.)) milk from indoor use of DDT and HCH in malaria control programmes. Science of the Total Environment 86: 281–87. DOI: https://doi.org/10.1016/0048-9697(89)90290-8

Campagna C, Sirard M A, Ayotte P and Bailey J L. 2001. Impaired maturation, fertilization, and embryonic development of porcine oocytes following exposure to an environmentally relevant organochlorine mixture. Biology of Reproduction 65: 554–60. DOI: https://doi.org/10.1095/biolreprod65.2.554

Curtis C F and Lines J D. 2000. Should DDT be banned by international treaty? Parasitology Today 16: 119–21. DOI: https://doi.org/10.1016/S0169-4758(99)01605-1

Ghuman S P S, Ratnakaran U, Bedi J S and Gill J P S. 2012. Impaired fertility in dairy animals in the backdrop of high pesticide contamination: a perspective. Proceedings of International Symposium on ‘One Health: Way Forward to Challenges in Food Safety and Zoonoses in 21st Century’ and XI Annual Conference of Indian Association of Veterinary Public Health Specialists (IAVPHS). Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana in collaboration with University of Saskatchewan, Canada, from 13–14 December 2012.

Ghuman S P S, Ratnakaran U, Bedi J S and Gill J P S. 2013. Impact of pesticide residues on fertility of dairy animals: A Review. Indian Journal of Animal Sciences 83: 1243–55.

Kamarianos A, Karamanlis X, Goulas P, Theodosiadou E and Smokovitis A. 2003. The presence of environmental pollutants in the follicular fluid of farm animals (cattle, sheep, goats, and pigs). Reproductive Toxicology 17: 185–90. DOI: https://doi.org/10.1016/S0890-6238(02)00118-1

Mathur H B, Agarwal H C, Johnson S and Saikia N. 2005. Analysis of pesticide residues. Blood Samples from Villages of Punjab, Centre for Science and Environment, New Delhi. www.cseindia.org.

Mills P A, Bong B A, Kamps L A and Burke J A. 1972. Elution solvent system for florisil column clean up in organochlorine pesticide residues analysis. Journal Association of Official Analytical Chemist 55: 39–43. DOI: https://doi.org/10.1093/jaoac/55.1.39

Moreno F M, Jimenez T M, Garrido F A, Martinez V J L, Olea S F and Olea N. 2004. Determination of organochlorine compounds in human biological samples by GC-MS/MS. Biomedical Chromatography 18 (Suppl 2): 102–11. DOI: https://doi.org/10.1002/bmc.300

Petro E M L, Adrian C, Leroy J L M R, Dirtu A C, Coen W D and Bols P E J. 2010. Occurrence of endocrine disrupting compounds in tissues and body fluids of Belgian dairy cows and its implications for the use of the cow as a model to study endocrine disruption. Science of the Total Environment 408: 5423–28. DOI: https://doi.org/10.1016/j.scitotenv.2010.07.051

Pocar P, Brevini T A, Perazzoli F, Cillo F, Modina S and Gandolfi F. 2001. Cellular and molecular mechanisms mediating the effects of polychlorinated biphenyls on oocyte developmental competence in cattle. Molecular Reproduction and Development 60: 535–41. DOI: https://doi.org/10.1002/mrd.1118

Ratnakaran U, Ghuman S P S, Bedi J S and Gill J P S. 2012. Reproductive status of bovines’ vis à-vis serum concentrations of pesticide residues. Proceedings of 28th Annual Convention of ISSAR and National Symposium on ‘Addressing animal reproductive stresses through biotechnological tools. Khanapara, Guwahati, Asom from November 21–23, 2012. pp. 364–65.

Rolland M, Le Moal J, Wagner V, Royere D, and De Mouzon J. 2013. Decline in semen concentration and morphology in a sample of 26,609 men close to general population between 1989 and 2005 in France. Human Reproduction 28: 462–70. DOI: https://doi.org/10.1093/humrep/des415

Toppari J, Larsen J C, Christiansen P, Giwercman A, Grandjean P, Guillette L J, Jegou B, Jensen T K, Jouannet P, Keiding N, Leffers H, McLachlan J A, Meyer O, Muller J, Rajpert- De Meyts E, Scheike T, Sharpe R, Sumpter J and Skakkebaek N E. 1996. Male reproductive health and environmental xenoestrogens. Environmental Health Perspectives 104 (Suppl 4): 741–803. DOI: https://doi.org/10.1289/ehp.96104s4741

UNIDO. 2009. UNIDO Green Industry Newsletter http://www.unido.org/fileadmin/usermedia/Publications/Newsletter/Green Industry. Issued 4, April 2009.

USEPA. 2003. EPA Test Methods. In http: /www.epa.gov.

Zama A M and Uzumcu M. 2010. Epigenetic effects of endocrine- disrupting chemicals on female reproduction: An ovarian perspective. Frontiers in Neuroendocrinology 31: 420. DOI: https://doi.org/10.1016/j.yfrne.2010.06.003