Use of chelating agent for optimum post thaw quality of buck semen
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https://doi.org/10.56093/ijans.v84i8.43215
Keywords:
Acrosome, Buck semen, Cryopreservation, Egg yolk, EDTA, Glycerol, HOSAbstract
Ejaculates (35) from adult Sirohi bucks (2–4 years old) were utilized for the present study to find out the freezability of buck semen at different levels of chelating agent used (ethylene diamine tetra acetic acid - EDTA: 0, 0.01, 0.05 and 0.1%) by conventional method of freezing. The ejaculates were collected twice at weekly intervals using artificial vagina and were extended to maintain sperms concentration approximately 100 million / dose (0.25 ml) with tris- citric acid- fructose (TCF) diluent having 10% (v/v) egg yolk and 6% (v/v) glycerol as cryo protecting agent. Filling and sealing of straws were done at 5ºC in cold handing cabinet after 4 h of equilibration period then straws were vapor frozen for 10 min above 2 cm of liquid nitrogen and finally put in to liquid nitrogen. Post thaw motility, live sperm count, abnormalities, acrosomal integrity and hypo osmotic swelling test had been conducted to know freezability. Analysis of data using SPSS 16 revealed that post thaw motility, live sperm count, abnormalities, acrosomal integrity and hypo osmotic swelling positive spermatozoa differed significantly at different levels of EDTA. The post thaw motility, live sperm count, acrosomal integrity and hypo osmotic swelling positive spermatozoa were significantly highest in 0.1% of EDTA used in the present study. So, 0.01% EDTA can be used as an additive in semen dilutor in routine freezing process for better post thaw recovery of buck semen.
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References
Bailey J L and Buhr M M. 1995. Regulation of internal Ca2+ by chilled bull and boar spermatozoa. Cryobiology 32: 259–69. DOI: https://doi.org/10.1006/cryo.1995.1025
Bakas L S and Disalvo E A.1991. Effect of Ca2+ on the cryoprotective action of trehalose. Cryobiology 28: 347–53. DOI: https://doi.org/10.1016/0011-2240(91)90041-L
Hancock J L. 1951. A staining technique for the study of temperature shock in semen. Nature (London). 167: 323. DOI: https://doi.org/10.1038/167323b0
Khalifa T A A and El-Saidy B E. 2006. Pellet-freezing of Damascus goat semen in a chemically defined extender. Animal Reproduction Science 93: 303–15. DOI: https://doi.org/10.1016/j.anireprosci.2005.08.008
Mann T. 1964. The biochemistry of semen and of the male reproductive tract. London, Methuen.
Ranjan R, Ramachandran N, Jindal S K and Sinha N K. 2009a. Hypo osmotic swelling test in frozen thawed goat spermatozoa. Indian Journal of Animal Sciences 79 (10): 1022–23.
Ranjan R, Ramachandran N, Jindal S K, Sinha N K, Goel A K, Kharche S D and Sikarwar A K S. 2009b. Effect of egg yolk levels on keeping quality of Marwari buck semen at refrigeration temperature. Indian Journal of Animal Sciences 79 (7): 662–64.
Ritar A J and Salamon S. 1991. Effect of month of collection, method of processing, concentration of egg yolk and duration of frozen storage on viability of Angora goat spermatozoa. Small Ruminant Research 4: 29–37. DOI: https://doi.org/10.1016/0921-4488(91)90050-Z
Roldan E R S, Murase T and Shi Q X. 1994. Exocytosis in spermatozoa in response to progesterone and zona pellucid. Science 266: 1578–81. DOI: https://doi.org/10.1126/science.7985030
Roy A. 1957. Egg yolk-coagulating enzyme in the semen and Cowper’s gland of the goat. Nature 179: 318–19. DOI: https://doi.org/10.1038/179318b0
Salamon S and Maxwell W M C. 1995. Frozen storage of ram semen I. Processing, freezing, thawing and fertility after cervical insemination. Animal Reproduction Science 37: 185– 249. DOI: https://doi.org/10.1016/0378-4320(94)01327-I
Shannon P and Curson B. 1972. Toxic effect and mode of action of dead sperm on diluted bovine semen. Journal of dairy science 55: 614–20. DOI: https://doi.org/10.3168/jds.S0022-0302(72)85544-9
Shannon P and Curson B. 1982. Kinetics of the aromatic L amino- acid oxidase and the effect of catalase on fertility of diluted bovine semen stored at 5°C and ambient temperatures. Journal of reproduction and fertility 64: 463–68. DOI: https://doi.org/10.1530/jrf.0.0640463
Shannon P and Curson B. 1983.Effects of cysteine and EDTA on in vitro survival at 37 °C and fertility of diluted bovine semen. New Zealand Journal of Agricultural Research 26: (1) 85–88. DOI: https://doi.org/10.1080/00288233.1983.10420955
Tuli R K and Holtz W. 1995. Effect of season on the freezability of Boer goat semen in the northern temperate zone. Theriogenology 43: 1359–63. DOI: https://doi.org/10.1016/0093-691X(95)00120-W
Watson P F. 1990. Artificial insemination and the preservation of semen. In: Lamming GE (ed.), Marshall’s Physiology of Reproduction. Churchill Livingstone, Edinburgh 747–869.
Watson P F. 1975. Use of Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa. Veterinary Records 97: 12–15. DOI: https://doi.org/10.1136/vr.97.1.12
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