Disturbance of oxidant/antioxidant balance, acute phase response and high mobility group boxâ€“1 protein in acute undifferentiated diarrhea in crossbred piglets

U K DE; REENA MUKHERJEE; S NANDI; BHARAT BHUSHAN; U DIMRI; A K VERMA

doi:10.56093/ijans.v84i8.43266

Authors

U K DE Indian Veterinary Research Institute,Izatnagar, Uttar Pradesh 243 122 India
REENA MUKHERJEE Indian Veterinary Research Institute,Izatnagar, Uttar Pradesh 243 122 India
S NANDI Indian Veterinary Research Institute,Izatnagar, Uttar Pradesh 243 122 India
BHARAT BHUSHAN Indian Veterinary Research Institute,Izatnagar, Uttar Pradesh 243 122 India
U DIMRI Indian Veterinary Research Institute,Izatnagar, Uttar Pradesh 243 122 India
A K VERMA Indian Veterinary Research Institute,Izatnagar, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v84i8.43266

Keywords:

Ceruloplasmin, Haptoglobin, HMBG1, Oxidative stress, Piglet diarrhoea

Abstract

The objective of the present study was to investigate the status of high mobility group box–1 (HMGB1) protein, oxidative stress and acute phase proteins in natural cases of acute undifferentiated diarrhoea in piglets aged 1–15 days old. The study was conducted on 30 crossbred (Landrace × indigenous) piglets; fifteen suffering from acute enteritis (group 1) and fifteen healthy piglets as control (group 2). The diarrhoea was diagnosed on the basis of clinical symptoms. From the results of the study, it is concluded that HMGB1 protein, markers of oxidative stress and acute phase proteins might play important roles in the pathophysiology of piglet diarrhoea and that these may be targets for supportive therapy.

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References

Argenzio R A and Rhoads J M. 1997. Reactive oxygen metabolites in piglet cryptosporidiosis. Pediatric Research 41: 521–26. DOI: https://doi.org/10.1203/00006450-199704000-00011

Chen H H, Lin J H, Fung H P, Ho L L, Yang P C, Lee W C, Lee Y P and Chu R M. 2003. Serum acute phase proteins and swine health status. The Canadian Journal of Veterinary Research 67: 283–90.

Dai S, Sodhi C, Cetin S, Richardson W, Branca M, Neal M D, Prindle T, Ma C, Shapiro R A, Li B, Wang J H and Hackam D J. 2010. Extracellular high mobility group box–1 (HMGB1) inhibits enterocyte migration via activation of Toll-like receptor-4 and increased cell-matrix adhesiveness. Journal of Biological Chemistry 285: 4995–5002. DOI: https://doi.org/10.1074/jbc.M109.067454

Daudelin J F, Lessard M, Beaudoin F, Nadeau É, Bissonnette N, Boutin Y, Brousseau J P, Lauzon K, Fairbrother J M, 2011. Administration of probiotics influences F4 (K88)-positive enterotoxigenic Escherichia coli attachment and intestinal cytokine expression in weaned pigs. Veterinary Research 42: 69. DOI: https://doi.org/10.1186/1297-9716-42-69

Draper H H and Hadley M. 1990. Malondialdehyde determination as index of lipid peroxidation. Methods in Enzymology 186: 421–31. DOI: https://doi.org/10.1016/0076-6879(90)86135-I

Grau-Roma L, Heegaard P M, Hjulsager C K, Sibila M, Kristensen C S Allepuz A, Piñeiro M, Larsen L E, Segalés J and Fraile L. 2009. Pig-major acute phase protein and haptoglobin serum concentrations correlate with PCV2 viremia and the clinical course of postweaning multisystemic wasting syndrome. Veterinary Microbiology 138: 53–61. DOI: https://doi.org/10.1016/j.vetmic.2009.03.005

Green E M and Adams H R. 1992. New perspectives in circulatory shock: pathophysiologic mediators of the mammalian response to endotoxemia and sepsis. Journal of the American Medical Association 200: 1834–41.

Jeong K I, Zhang Q, Nunnari J and Tzipori S. 2010. A piglet model of acute gastroenteritis induced by Shigella dysenteriae Type 1. Journal of Infectious Diseases 201: 903–11. DOI: https://doi.org/10.1086/650995

Katsuda K, Kohmoto M, Kawashima K and Tsunemitsu H. 2006.Frequency of enteropathogen detection in suckling and weaned pigs with diarrhea in Japan. Journal of Veterinary Diagnostic Investigation 18: 350–54. DOI: https://doi.org/10.1177/104063870601800405

Keel M K and Songer J G. 2006. The Comparative Pathology of Clostridium difficile -associated Disease. Veterinary Pathology 43: 225–40. DOI: https://doi.org/10.1354/vp.43-3-225

Kim Y J, Kim E H and Hahm K B. 2012. Oxidative stress in inflammation-based gastrointestinal tract diseases: challenges and opportunities. Journal of Gastroenterology and Hepatology 27: 1004–10. DOI: https://doi.org/10.1111/j.1440-1746.2012.07108.x

Kono H and Rock K L. 2008. How dying cells alert the immune system to danger. Nature Reviews Immunology 8: 279–89. DOI: https://doi.org/10.1038/nri2215

Lobo V, Patil A, Phatak A and Chandra N. 2010. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Review 4: 118–26. DOI: https://doi.org/10.4103/0973-7847.70902

Luan Z G, Zhang H, Ma X C, Zhang C and Guo R X. 2010. Role of high-mobility group box 1 protein in the pathogenesis of intestinal barrier injury in rats with severe acute pancreatitis. Pancreas 39: 216–23. DOI: https://doi.org/10.1097/MPA.0b013e3181bab5c5

Lubos E, Handy D E and Loscalzo J. 2008. Role of oxidative stress and nitric oxide in atherothrombosis. Frontiers in Bioscience 13: 5323–44. DOI: https://doi.org/10.2741/3084

Moen S T, Blumentritt C A, Slater T M, Patel S D, Tutt C B, Estrella-Jimenez M E, Pawlik J, Sower L, Popov V L, Schein C H, Gilbertson S R, Peterson J W and Torres A G. 2010. Testing the Efficacy and Toxicity of Adenylyl Cyclase Inhibitors against Enteric Pathogens Using In Vitro and In Vivo Models of Infection. Infection and Immunity 78: 1740–49. DOI: https://doi.org/10.1128/IAI.01114-09

Pelletier N, Boudreau F, Yu S J, Zannoni S, Boulanger V and Asselin C. 1998. Activation of haptoglobin gene expression by cAMP involves CCAAT/enhancer-binding protein isoforms in intestinal epithelial cells. FEBS Letters 439: 275–80. DOI: https://doi.org/10.1016/S0014-5793(98)01388-X

Petersen H H, Nielsen J P and Heegard P M H. 2004. Application of acute phase protein measurements in veterinary clinical chemistry. Veterinary Research 35: 163–87. DOI: https://doi.org/10.1051/vetres:2004002

Ranjan R, Naresh R, Patra R C and Swarup D. 2006. Erythrocyte lipid peroxides and blood zinc and copper concentrations in acute undifferentiated diarrhoea in calves. Veterinary Research Communication 30: 249–54. DOI: https://doi.org/10.1007/s11259-006-3185-8

Shea-Donohue T, Fasano A, Smith A and Zhao A. 2010. Enteric pathogens and gut function: Role of cytokines and STATs. Gut Microbes 1: 5: 316–24. DOI: https://doi.org/10.4161/gmic.1.5.13329

Snedecor G W and Cochran W G. 1994. Statistical methods, (Iowa State University Press, Ames, Iowa, USA).

Soneja A, Drews M and Malinski T. 2005. Role of nitric oxide, nitroxidative and oxidative stress in wound healing. Pharmacological Reports 57 Suppl: 108–19.

Splichalova A, Splichal I, Chmelarova P and Trebichavsky I. 2011. Alarmin HMGB1 is released in the small intestine of gnotobiotic piglets infected with enteric pathogens and its level in plasma reflects severity of sepsis. Journal of Clinical Immunology 31: 488–97. DOI: https://doi.org/10.1007/s10875-010-9505-3

Stadnyk A W and Waterhouse C C M. 1997. Epithelial cytokines in intestinal inflammation and mucosal immunity Current Opinion in Gastroenterology 13: 510–17. DOI: https://doi.org/10.1097/00001574-199711000-00012

Suffredini A F, Fantuzzi G, Badolato R, Oppenheim J J and O’grady N P. 1999. New insights into the biology of the acute phase response. Journal of Clinical Immunology 19: 203–14. DOI: https://doi.org/10.1023/A:1020563913045

Sunderman S W Jr and Nomoto S. 1970. Measurement of human serum ceruloplasmin by its p-phenylenediamine oxidase DOI: https://doi.org/10.1093/clinchem/16.11.903

activity. Clinical Chemistry 16: 903–10.

Vitali R, Stronati L, Negroni A, Di Nardo G, Pierdomenico M, del Giudice E, Rossi P and Cucchiara S. 2011. Fecal HMGB1 is a novel marker of intestinal mucosal inflammation in pediatric inflammatory bowel disease. American Journal of Gastroenterology 106: 2029–40. DOI: https://doi.org/10.1038/ajg.2011.231

Wang H, Ward M F, Fan X G, Sama A E and Li W. 2006. Potential role of high mobility group box 1 in viral infectious diseases. Viral Immunology 19: 3–9. DOI: https://doi.org/10.1089/vim.2006.19.3

Yang H, Ochani M, Li J, Qiang X, Tanovic M, Harris H E, Susarla S M, Ulloa L, Wang H, DiRaimo R, Czura C J, Wang H, Roth J, Warren H S, Fink M P, Fenton M J, Andersson U and Tracey K J. 2004. Reversing established sepsis with antagonists of endogenous high-mobility group box 1. Proceedings of National Academy of Science USA 101, 296–30. DOI: https://doi.org/10.1073/pnas.2434651100

Yucel A A, Gulen S, Dincer S, Yucel A E and Yetkin G I. 2012. Comparison of two different applications of the Griess method for nitric oxide measurement. Journal of Experimental and Integrative Medicine 2: 167–71. DOI: https://doi.org/10.5455/jeim.200312.or.024

Zadrozny L M, Stauffer S H, Armstrong M U, Jones S L and Gookin J L. 2006. Neutrophils Do Not Mediate the Pathophysiological Sequelae of Cryptosporidium parvum Infection in Neonatal Piglets. Infection and Immunity 74: 5497–505. DOI: https://doi.org/10.1128/IAI.00153-06

Zyczko K and Zyczko G M. 2005. Effects of transferrin and ceruloplasmin on the resistance of sucking piglets to Escherichia coli strains causing diarrhea. Medycyna Weteryneryjna 61: 426–29.