Quality assessment of yak oocytes for in vitro production of yak embryos

M HUSSAIN; P CHAKRAVARTY; M S CHAUHAN; S SOREN; B K D BORAH; J DOLEY; P J DAS

doi:10.56093/ijans.v84i9.43526

Authors

M HUSSAIN NDRI, Karnal, Haryana
P CHAKRAVARTY Assam Agricultural University, Khanapara, Asom
M S CHAUHAN Assam Agricultural University, Khanapara, Asom
S SOREN Assam Agricultural University, Khanapara, Asom
B K D BORAH Assam Agricultural University, Khanapara, Asom
J DOLEY Assam Agricultural University, Khanapara, Asom
P J DAS Assam Agricultural University, Khanapara, Asom

https://doi.org/10.56093/ijans.v84i9.43526

Keywords:

Embryo manipulation, Fertilization, In vitro maturation, Oocytes, Yak

Abstract

Yak oocytes were isolated and morphologically classified into 3 categories, i.e. good, fair and poor quality oocytes according to the compactness of the cumulus cells, and were assigned for in vitro maturation, fertilization and embryonic development. Oocytes were retrieved by both aspiration and post-aspiration slicing technique, and the overall recovery rates of good, fair and poor quality oocytes were estimated at 46.55, 30.6 and 22.84% respectively. Maturation rates (M II) in good quality oocytes were significantly higher as compared to that in fair and poor quality oocytes. Total and normal fertilization rates were also higher in good quality oocytes than in fair and poor oocytes. Good quality oocytes led to embryonic development with significant numbers of transferrable embryos (i.e. morula and blastocyst); 60% of morula was further developed to blastocyst. Thus, it is concluded that the selection of good oocytes according to cumulus cells compactness is an important criterion for in vitro maturation, fertilization, and embryonic development in yaks.

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References

Bavister B D. 2002. Early history of in vitro fertilization. Reproduction 124: 181–96. DOI: https://doi.org/10.1530/rep.0.1240181

Brackett B G and Oliphant G. 1975. Capacitation of rabbit spermatozoa in vitro. Biology of Reproduction 12: 260–74. DOI: https://doi.org/10.1095/biolreprod12.2.260

Chauhan M S, Singla S K, Palta P, Manik R S and Madan M L. 1999. Effect of epidermal growth factor on the cumulus expansion, meiotic maturation and development of buffalo oocytes in-vitro. Veterinary Record 144: 266–67. DOI: https://doi.org/10.1136/vr.144.10.266

Downs S M, Schroeder A C and Eppig J J. 1986. Serum maintains the fertilizability of mouse oocytes matured in vitro by preventing the hardening of the zona-pellucida. Gamete Research 15: 115–22. DOI: https://doi.org/10.1002/mrd.1120150203

Fukui Y. 1990. Effect of follicle cell on the acrosome reaction, fertilization and development competence of bovine oocytes matured in vitro. Molecular Reproduction and Development 26: 40–46. DOI: https://doi.org/10.1002/mrd.1080260107

Guo X, Ping Y, Yufeng X and Jinxing L. 2006. White yak oocyte collection methods and storage condition on in-vitro maturation of ovarian influence. Journal of Animal Sciences and Veterinary Medicine 25 (6): 13–15.

Im K S, Kim H J, Chung K M, Kim H S and Park K W. 1995. Effect of ovary type, oocyte grade, hormone, sperm concentration and fertilization medium on in vitro maturation, fertilization and development of bovine follicular oocytes. American Journal of Animal Sciences 8 (2): 123–27. DOI: https://doi.org/10.5713/ajas.1995.123

Kumar S and Maurya S N. 2000. Evaluation of oocytes retrival methods from bubaline ovaries. Indian Journal of Animal Research 34 (2): 130–32.

Pawshe C H. 2010. In vitro maturation, fertilization and embryonic development of morphological classified goat oocytes. Indian Journal of Animal Sciences 80 (110): 1080–83.

Pavlok A, Lucas H A and Niemann H. 1992. Fertilization and development competence of bovine oocytes derived from different categories of antral follicles. Molecular Reproduction and Development 31: 63–67. DOI: https://doi.org/10.1002/mrd.1080310111

Trounson A. 1992. The production of ruminant embryos of in vitro of bovine oocytes. Animal Reproduction Sciences 28: 125–37. DOI: https://doi.org/10.1016/0378-4320(92)90099-Y

Zi X D, Yin R H, Chen S W, Liang G N, Zhang D W and Guo C H. 2009. Development competence of embryos derived from reciprocal in vitro fertilization between yak (Bos grunniens) and cattle (Bos taurus). Journal of Reproduction and Development 55 (5): 480–83. DOI: https://doi.org/10.1262/jrd.20202

Zi X D, Zhong G H, Wen Y L, Zhong J C, Liu C L, Ni Y A, Yezi Y H and Ashi M G. 2004. Growth performance, carcass composition and meat quality of Jiulong-yak (Bos grunniens). Asian-Australasian Journal of Animal Science 17: 410–14. DOI: https://doi.org/10.5713/ajas.2004.410