Methylene blue reduction test in semen analysis of White Pekin ducks

A K NAHAK; S C GIRI; P C MISHRA; D N MOHANTY; S K DASH

doi:10.56093/ijans.v84i10.44264

Authors

A K NAHAK NRC on Mithun, Jharnapani, Medziphema
S C GIRI CARI, Regional Centre, Bhubaneshwar
P C MISHRA Orissa University of Agriculture and Technology, Bhubaneshwar, Odisha 751 003 India
D N MOHANTY Orissa University of Agriculture and Technology, Bhubaneshwar, Odisha 751 003 India
S K DASH Orissa University of Agriculture and Technology, Bhubaneshwar, Odisha 751 003 India

https://doi.org/10.56093/ijans.v84i10.44264

Keywords:

MBRT, Semen parameters, White Pekin Duck

Abstract

This study was undertaken to investigate the relationship between methylene-blue reduction time (MBRT) and other semen quality parameters in duck by analysis of correlation coefficients. Semen ejaculates (32), collected at 2 days interval from 10 randomly selected drakes, were examined for different seminal parameters like pH, volume, colour, mass activity, individual motility, livability, concentration, number of spermatozoa per ejaculate, total sperm abnormality per cent and MBRT. The overall mean time to decolourise methylene blue in the white Pekin ducks was 10.03±0.24 min with a range from 8 to 13 min. MBRT was negatively correlated with mean spermatozoa concentration, number of spermatozoa per ejaculation and colour of semen samples but positively correlated with individual motility and livability. High correlation of MBRT with seminal parameters indicated its practical utility in monitoring semen characteristics.

Downloads

Download data is not yet available.

References

Bratton R W, Foote R H, Henderson C R, Musgrave S D, Dunbar R S Jr and Dunn H O.1956. The relative usefulness of combinations of laboratory tests for predicting the fertility of bovine semen. Journal of Dairy Science 39: 1542–9. DOI: https://doi.org/10.3168/jds.S0022-0302(56)94887-1

Burrows W H and Quinn J P. 1936. Artificial insemination in fowls. Journal of Heredity 27: 31–37. DOI: https://doi.org/10.1093/oxfordjournals.jhered.a104138

Cyriac S, Joseph L, Peethambaran P A, Narayanankutty K and Karthiayini K. 2013. Semen quality characteristics of White Pekin, Kuttanad (Anas platyrhynchos domesticus) and Muscovy (Cairina moschata momelanotus) drakes. Indian Journal of Animal Sciences 83 (6): 595–99.

Etuk I F, Ojewola G S and Nwachukwu E N. 2006. Effect of management systems on semen quality of Muscovy drakes. International Journal of Poultry Science 5: 482–84. DOI: https://doi.org/10.3923/ijps.2006.482.484

Ghonim A I A, Awad A L, El.sawy A M, Fatouh M H and Ibraheim Z A. 2009. Effect of frequency of semen collection, dilution rate and insemination dose on semen characteristics and fertility of Domyati ducks. Egyptian Journal of Poultry Science 29: 1023–1045.

Ghonim A I A, Awad A L, Elkloub K and El. Moustafa M. 2010. Effect of feeding different levels of energy and crude protein on semen quality and fertility of Domyati ducks. Egyptian Journal of Poultry Science 30: 583–600.

Herman H A and Madden F W. 1953. The Artificial Insemination of Dairy Cattle. Lucas Bros. Publ., Columbia, MO, USA.

Jones J E and Wilson H R. 1967. Use of an electronic counter for sperm concentration determination in chicken semen. Poultry Science 46: 532–33. DOI: https://doi.org/10.3382/ps.0460532

Nair S C. 2003. ‘Effect of blue green algae (Spirulina platensis) on haematological, biochemical and fertility parameters of egg type male chicken.’ M.V.Sc. Thesis. Kerala Agricultural University.

Pistenma P A, Snapir N and Mel H C. 1971. Biophysical characterization of fowl spermatozoa. 1. Preservating of motility and fertilizing capacity under conditions of low temperature and low sperm concentration. Journal of Reproduction and Fertility 24:153–60. DOI: https://doi.org/10.1530/jrf.0.0240153

Saxena, M. S. 2000. Veterinary Andrology and Artificial Insemination. 1st edn, pp. 89–94. CBS publishers and distributors, New Delhi, India.

Sharma P V. 1995. A simplified staining technique for evaluation of acrosomal status of sperm cells. Indian Journal of Animal Reproduction 16: 127.

Tardif A L, Farrell P B, Trouern-Trend V, Simkin M E, Foote R H. 1998. Use of Hoechst 33342 stain to evaluate live fresh and frozen bull sperm by computer assisted analysis. Journal of Andrology 19: 201–6. DOI: https://doi.org/10.1002/j.1939-4640.1998.tb01989.x

VanDemark N L, Mercier E and Salisbury G W. 1945. The methylene blue reduction test and its relation to other measures of quality in bull semen. Journal of Dairy Science 28: 121–8. DOI: https://doi.org/10.3168/jds.S0022-0302(45)95152-6

Zemjanis R. 1970. Collection and evaluation of semen. Diagnostic and Therapeutic techniques in Animal Reproduction. 2nd edn. Williams and Wilkins Co., Baltimore MD pp. 139–56.