DNA barcoding of commonly prevalent Culicoides midges in South India

ARCHANA M; PLACID E D SOUZA; S K JALALI; C RENUKAPRASAD; RAKSHITH OJHA

doi:10.56093/ijans.v85i1.46154

Authors

ARCHANA M PhD Scholar, Karnataka Veterinary, Animal and Fisheries Science University, Hebbal, Bengaluru, Karnataka 560 024 India
PLACID E D SOUZA Professor and Head, Department of Parasitology, Veterinary College, Karnataka Veterinary, Animal and Fisheries Science University, Hebbal, Bengaluru, Karnataka 560 024 India
S K JALALI Principal Scientist, Karnataka Veterinary, Animal and Fisheries Science University, Hebbal, Bengaluru, Karnataka 560 024 India
C RENUKAPRASAD Vice Chancellor, Karnataka Veterinary, Animal and Fisheries Science University, Hebbal, Bengaluru, Karnataka 560 024 India
RAKSHITH OJHA SRF, National Bureau of Agriculturally Important Insects, Hebbal, Bengaluru

https://doi.org/10.56093/ijans.v85i1.46154

Keywords:

Bold Systems v3, CuliCOIdes, Cytochrome oxidase I, DNA barcoding

Abstract

DNA barcoding has gained increased recognition as a molecular tool for species identification of insects. Interspecific variation in DNA sequences of some genes is much higher than intraspecific and provides an opportunity to use DNA sequences for species identification. A study was therefore undertaken to barcode 5 commonly prevalent CuliCOIdes species in farming regions of Bengaluru districts in Karnataka state. The barcoding of Cytochrome oxidase I (COI) gene of C. anophelis, C. palpifer, C. huffi, C. innoxius and C. circumscriptus yielded an amplified fragment of 648 bp sequence. Barcode for all 5 species was generated using BoldSystems v3 and submitted to genbank for accession numbers. DNA barcoding enabled exact identification of 5 prevalent species.

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