Egg yolk powder an alternative to fresh-egg yolk for buffalo semen cryopreservation

MAHAK SINGH; NIRMAL CHANDRA BARIK; S K GHOSH; J K PRASAD; J S RAJORIA; Y K SONI; ASHOK KUMAR; J K CHAUDHARY; N SRIVASTAVA

doi:10.56093/ijans.v85i1.46163

Authors

MAHAK SINGH Scientist, Indian Council of Agricultural Research (Regional Centre) for NEH Region, Barapani
NIRMAL CHANDRA BARIK M.V.Sc. Scholar, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122 India
S K GHOSH Principal Scientist, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122 India
J K PRASAD Senior Scientist,Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122 India
J S RAJORIA Ph.D. Scholar, Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122 India
Y K SONI Scientist, Central Institute for Research on Cattle, Meerut
ASHOK KUMAR Scientist, Indian Council of Agricultural Research (Regional Centre) for NEH Region, Barapani
J K CHAUDHARY Ph.D. Scholar, Germ Plasm Centre, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122 India
N SRIVASTAVA Scientist, Central Institute for Research on Cattle, Meerut

https://doi.org/10.56093/ijans.v85i1.46163

Keywords:

Buffalo semen, Cryopreservation, Egg yolk powder, Semen, Sperm

Abstract

The present work was carried out to evaluate the effect of commercial egg yolk (EY) powder as an alternative to fresh EY on buffalo semen freezability. Ejaculates (12) from 3 bulls (4 from each bull) were collected as per standard procedure. After initial selection of ejaculates on the basis of mass activity (+3 and above) and individual progressive motility (70% and above), ejaculates were divided into 3 parts and diluted with tris dilutor, G1, containing 5% EY powder; G2, containing 10% EY powder, and G3, containing fresh EY (20%). Ejaculates were processed and cryopreserved using automated biological cell freezer followed by examination of frozen samples after 24 h. Semen quality variables (individual progressive motility, viability using propidium iodide, acrosome integrity using FITC-PSA and membrane integrity by HOST) were examined at fresh and post-thaw stage. At fresh stage semen quality variables did not vary among 3 groups.

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