Molecular characterization of field isolates of Pasteurella multocida from Poultry

Abstract

Study reports isolation and molecular characterization of P. multocida from poultry by using peR and REA. Swabs (69) from 43 different poultry farms were processed and 5 cases were positive for P. multocida. All the P. multoicda isolates were oxidase, catalase positive, non-haemolytc and showed no growth on MLA. Biochemically isolates belonged . to P. multocida subsp. multocida. Isolates were sensitive to enrofloxacin, ciprofloxacin, pefloxacin and chloramphenicol. peR was positive for 5 infra-orbital sinus swabs. In REA using enzymes Hha I and Hpa II, the isolates revealed two distinct banding patterns.