Comparative the efficiency of different oocyte recovery methods from cattle ovaries

Abstract

The present study was undertaken in cattle to study the efficiency of 3 different methods of oocyte recovery. Slaughterhouse ovaries were collected and the oocytes were collected from the ovaries by aspiration, puncturing and slicing methods. Total 417 cattle ovaries were collected and 586 oocytes were recovered in different grades. The maximum number (27.93%) of A grade oocytes were recovered by puncturing method followed by slicing (23.05%) and aspiration method (20.69%). Slicing method showed the highest recovery rate per ovary i.e. 1.75 whereas puncturing method showed the lowest recovery rate per ovary (1.04). It can be concluded that slicing of ovary is the best oocyte recovery method.