Molecular characterization of Salmonella serovars of zoonotic importance*
Keywords:Animal faecal samples, Foods of animal origin, Human stool, Salmonella serovars, Virulence genes
Isolates (50) of Salmonella were recovered from 1,132 samples from foods of animal origin and fecal samples from animals and human beings; belonging to 10 different serovars with most prevalent serovar S. Typhimurium (21) followed by S. Weltevreden (12), S. Ughelli (5), S. Essen (3), S. Elisabethville (2), S. Lagos (2), S. Drogana (2), S. Enteritidis (1), S. London (1) and un-typable Salmonella (1). Emerging Salmonella serovars, viz S. Elisabethville, S. Essen, S. Lagos, S. Ughelli and S. Drogana were first time recovered from Pantnagar and its vicinity. S. Drogana was recovered possibly for the first time from human source in India. Multiple Salmonella serovars (up to 3 serovars comprising S. Typhimurium, S. Weltevreden and S. Essen) were recovered from single cattle dung sample while, multiple serovars (up to 2 serovars) were also recorded in many single samples, viz cattle dung (S. Weltevreden and S. Ughelli), poultry droppings (S. Essen and S. Ughelli), pig faeces (S. Weltevreden and S. London), sheep faeces (S. Typhimurium and S. Drogana) and pig faeces (S. Weltevreden and S. Ughelli). Different virulence genes, viz. invA, sipA, sefA, fliC, stn and sopB were detected in Salmonella isolates using PCR-based molecular technique. Among these virulence genes, invA gene was the most prevalent one as is present in 98% Salmonella isolates followed by sopB, stn, sipA, fliC and sefA genes in 96, 86, 78, 32 and 10% Salmonella isolates, respectively. Salmonella serovars of zoonotic importance recovered from varied sources exhibited different virulence enes that may cause serious infections in animals as well as in human beings. Ultimately these virulent serovars may pose great risks to the health and production in the animals and serious health hazards in human.
Aldridge P, Gnerer J, Karlinsey J E and Hughes K T. 2006. Transcriptional and translational control of the Salmonella fliC gene. Journal of Bacteriology 188: 4487–96.
CDC. 2006. Salmonella Surveillance: Annual Summary. 2006. Centre for disease Control and Prevention, Atlanta, Ga.
Chiu C H and Ou J T. 1996. Rapid identification of Salmonella serovars in faeces by specific detection of virulence genes invA and spvC by an enrichment broth culture-multiplex PCR combination assay. Journal of Clinical Microbiology 34: 2619– 22.
Cortez A L L, Carvalho A C F B, Ikuno A A, Burger K P and Vidal-Martins A M C. 2006. Identification of Salmonella species isolated from chicken abattoir by multiplex-PCR. Research in Veterinary Science 81(3): 340–44.
Darwin K H and Miller V L. 1999. Molecular basis of the interaction of Salmonella with the intestinal mucosa. Clinical Microbiology Review 12 (3): 405–28.
Hepfelmeier S, Ehabar K, Stecher B, Barthel M, Kremer M and Hardt W D. 2004. Role of Salmonella pathogenicity island 1 effecter protein sipA, sopB, sopE and sopE2 in Salmonella enterica subspecies 1 serovar Typhimurium colitis in streptomycin-pre-treated mice. Infection and Immunity 72 (2): 795–809.
ISO 6579: 2002(E). Microbiology - General Guidance on Methods for the Detection of Salmonella. 4th edn. International Organization for Standardization, Geneva, Switzerland.
Murugkar H V, Rahman H and Dutta P K. 2003. Distribution of virulence genes in Salmonella serovars isolated from man and animals. Indian Journal of Medical Research 117: 66–70.
Oliveira S D, Rodenbusch C R, Ce M C, Rocha S L S and Canal C W. 2003. Evaluation of selective and non-selective enrichment PCR procedures for Salmonella detection. Letters in Applied Microbiology 36: 217–21.
Prager R, Furth A and Tsachape H. 1995. Salmonella enterotoxin (stn) gene is prevalent in among strains of Salmonella enterica, but not among bongori and other Enterobacteriaceae. FEMS Immunology and Medical Microbiology 12: 47–50.
Rahman H, Prager R and Tschape H. 2000. Occurrence of sef and pef genes among different serovars of Salmonella. Indian Journal of Medical Research 111: 40–42.
Rahman H. 2006. Prevalence and phenotypic expression of sopB gene among clinical isolates of Salmonella enterica. Indian Journal of Medical Research 123: 83–88.
Sambrook J and Russell D W. 2001. Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Singh B R, Babu N, Jyoti J, Shankar H, Vijo T V, Agrawal R K, Chandra M, Kumar D and Teewari E. 2007. Prevalence of multi-drug-resistant Salmonella in equids maintained by low income individuals and on designated equine farms in India. Journal of Equine Veterinary Science 27(6): 266–76.
Skyberg J A, Louge C M and Nolan L K. 2006. Virulence genotyping of Salmonella spp. with multiplex PCR. Avian Diseases 50: 77–81.
Soumet C, Ermel G, Fach P and Colin P. 1994. Evaluation of different DNA extraction procedures for the detection of Salmonella from chicken products by polymerase chain reaction. Letters in Applied Microbiology 19: 294–98.
Swamy S C, Barnhart H M, Lee M D and Dreesen D W. 1996. Virulence determinants invA and spvC in salmonellae isolated from poultry products, wastewater and human sources. Applied and Environmental Microbiology 62: 3768–71.
USDA/FSIS. 2002. Isolation and identification of Salmonella from meat, poultry and egg products, MLG 4.02, rev. 10/25/02. USDA/FSIS Microbiology Laboratory Guidebook. 3rd edn. U.S. Department of Agriculture, Food Safety Inspection Service, Washington, DC
Wang W P, Li L, Shen J Z, Yang F J and Wu Y N. 2009. Quinolone resistance in Salmonella is associated with decreased mRNA expression of virulence genes invA and avrA, growth and intracellular invasion and survival. Veterinary Microbiology 133: 328–34.
WHO/CDC. 2003. Salmonella serotype Typhi. Manual for the laboratory identification of and anti-microbial susceptibility testing of bacterial pathogens of public health importance in the developing world.
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