Immune response of sheep to bentonite clay and alum-adjuvanted enterotoxaemia vaccines

Abstract

An investigation was undertaken to evaluate the immune response of sheep to 2 types of enterotoxaemia vaccines prepared by incorporating bentonite clay and alum separately as adjuvants. Clostridium perjringens type D was used for preparation of vaccines. Two groups of sheep were immunized separately with both vaccine fonnulations, and the immune response of sheep against all the formulations was assayed by mouse neutralization test (MNT). It was evident from the result that both types of vaccines protected sheep against enterotoxaemia up to 120th day post vaccination (MNT titre of 2 IU/mI) without booster dose of vaccination. Bentonite clay adjuvanted enterotoxaemia vaccine was found superior to alum precipitated vaccine in terms of cost, availability and production of higher immune response at 30th day of post vaccination.