Effect of supplementing certain vitamins and chelated trace minerals on reducing incidence of sub-clinical mastitis in crossbred cows

B M BHANDERI; M R GARG; AJAY GOSWAMI

doi:10.56093/ijans.v85i2.46612

Authors

B M BHANDERI Scientist-II, National Dairy Development Board, Anand, Gujarat 388 001 India
M R GARG General Manager, National Dairy Development Board, Anand, Gujarat 388 001 India
AJAY GOSWAMI Scientist-I, Animal Nutrition Group, National Dairy Development Board, Anand, Gujarat 388 001 India

https://doi.org/10.56093/ijans.v85i2.46612

Keywords:

Crossbred cows, Minerals, Somatic cell counts, Sub-clinical mastitis, Vitamin E

Abstract

Crossbred cows (93) having a history of sub-clinical mastitis (SCM) and clinical mastitis (CM) in previous lactation were selected from 57 farms in Sabarkantha district of Gujarat. Twenty crossbred cows from 12 farms served as control, and the remaining (n=73) were fed daily 10 g supplement per animal containing coated vitamins E and A, chelated copper (Cu), zinc (Zn), chromium (Cr), along with iodine(I), for 4 weeks prior to calving. Milk samples were collected post calving on day 10, 30, 60 and 90 for analysis of somatic cell counts (SCC), sodium (Na), potassium (K), chloride (Cl) content, pH and electrical conductivity (EC). Blood samples were also collected for neutrophil count, measurement of immunoglobulin and ferric reduction anti-oxidant power (FRAP). The milk producers were provided with Mastect strip for weekly checkup of SCM. Out of 73 supplemented cows, only 15 (21%) showed signs of SCM and CM with Mastect strip and California mastitis test, which was confirmed by SCC in milk (average 7.26×105 cells/ml milk). However, 16 animals (80%) out of 20 in control group were affected by SCM as indicated by the SCC (average 10.11×105 cells/ml milk), which was later aggravated to CM. Milk pH, EC, Na and Cl content in milk were higher in animals affected by SCM than the normal animals. In supplemental group, SCC in 58 (79%) animals was within the normal range (1.22–2.36×105 cells/ml milk) and no signs of SCM or CM were observed. Milk lactose, protein, solids-not-fat (SNF) content and FRAP were higher in unaffected as compared to mastitis affected animals. On feeding the supplement, blood neutrophil count decreased, whereas, immunoglobulin and FRAP activity increased significantly (P<0.05). The inference could be drawn that feeding a vitamins and chelated minerals based supplement 4 weeks prior to calving could significantly help in reducing the incidence of SCM in crossbred cows.

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References

Benzie I F F and Strain J J. 1996. The ferric reducing ability of plasma (FRAP) on a measure of anti-oxidant power: The FRAP Assay. Analytical Biochemistry 239: 70–76. DOI: https://doi.org/10.1006/abio.1996.0292

Bhanderi B M and Garg M R. 2012. A study on reducing the incidence of sub-clinical and clinical mastitis in dairy cows by feeding a vitamins and minerals based strategic feed supplement. Indian Journal of Dairy Science 65 (5): 388–92.

Borucki Castro S I, Berthiaume R R, Robichaud A and Lacasse P. 2012. Effects of iodine intake and teat-dipping practices on milk iodine concentrations in dairy cows. Journal of Dairy Science 95 (1): 213–20. DOI: https://doi.org/10.3168/jds.2011-4679

Brooks I B, Luster G A and Easterly D B. 1996. A procedure for the rapid determination of the major cations in milk by Atomic Absorption Spectroscopy. Manual of Perkin Elmer Inc. no. 0303–0152.pp. 27–35.

Drake E A, Paape M J, DiCarlo A L, Leino L and Kapture J. 1992. Evaluation bulk tank milk samples. Proceedings of 31st Annual Meeting of National Mastitis Council, Arlington, VA., pp. 236–42.

Garg M R, Bhanderi B M and Gupta S K. 2008. Effect of supplementing certain chelated minerals and vitamins to overcome infertility in field animals. Indian Journal of Dairy Science 61 (1): 181–84.

Garg M R, Bhanderi B M, Sherasia P L and Goswami A. 2014. Recent concepts in mineral nutrition. Proceedings of Global Animal Nutrition Conference–2014 on “Climate Resilient Livestock Feeding Systems for Global Food Security” organized jointly by the ANSI, NIANP, CLFMA of India and VIV India during April 20–22, 2014 at Bangalore, pp. 113–20.

Harmon R J. 1994. Physiology of mastitis and factors affecting somatic cell count. Journal of Dairy Science 77: 2103–12. DOI: https://doi.org/10.3168/jds.S0022-0302(94)77153-8

Harmon R J and Torre P M. 1997. Economic implications of copper and zinc proteinates: role in mastitis control. Proceedings of the 13th Annual Symposium on Biotechnology in the Feed Industry. p. 419. (Eds.) Lyons T P and Jacques K A. Nottingham University Press, Loughborough, Leics, UK.

IDF. 1984. Recommended methods for somatic cell count in milk. Doc. No. 168, International Dairy Federation, Belgium, pp. 15–30.

Kellogg D W, Tomlinson D J, Socha M T and Johnson A B. 2004. Review: Effects of zinc methionine complex on milk production and somatic cell counts of dairy cattle: twelve-trial summary. Proceedings of Animal Science 20 (4): 295–301. DOI: https://doi.org/10.15232/S1080-7446(15)31318-8

Kellogg D W. 1990. Zinc methionine affects performance of lactating cows. Feedstuffs 62: 15.

NRC. 2001. Nutrient Requirements of Dairy Animals.7th edn. National Academy of Science – National Research Council, Washington, DC.

Persson K. 1992. ‘Studies on inflammation in the bovine teat with regard to its role in the defense against udder infections.’ Dissertation, Uppsala, Sweden.

Politis I, Hidiroglou M, Batra T R, Gilmore J A, Gorewit R C and Scherf H. 1995. Effects of vitamin E on immune function of dairy cows. American Journal of Veterinary Research 56: 179–84.

Politis I, Hidiroglou N, White J H, Gilmore J A, Williams S N, Scherf H and Frigg M. 1996. Effects of vitamin E on mammary and blood leukocyte function with emphasis on chemotaxis in peri-parturient dairy cows. American Journal of Veterinary Research 57: 468–71.

Popovic Z. 2004. ‘Performance and udder health status of dairy cows influenced by organically bound zinc and chromium.’ Ph.D. Thesis, University of Belgrade, Belgrade.

SAS. 2012. SAS Procedures Guide Release 9.3. edn. SAS Institute Inc.: Cary, NC, USA.

Sharma N. 2007. Alternative approach to control intra-mammary infection in dairy cows: A review. Asian Journal of Animal and Veterinary Advances 2 (2): 50–62. DOI: https://doi.org/10.3923/ajava.2007.50.62

Sharma N, Gautam A, Upadhyay S R, Hussain K, Soodan J S and Gupta S K. 2006. Role of antioxidants in udder health: a review. Indian Journal of Field Veterinarian 2 (1): 73–76.

Smith K L, Harrison J H, Hancock D D, Todhunter D A and Conrad H R. 1984. Effect of vitamin E and selenium supplementation on incidence of clinical mastitis and duration of clinical symptoms. Journal of Dairy Science 67: 1293. DOI: https://doi.org/10.3168/jds.S0022-0302(84)81436-8

Snedecor G W and Cochran W G. 1986. Statistical Methods. Oxford and IBH 678 Publishing Co., New Delhi.

Sordillo L M, Shafer-Weaver K and DeRosa D. 1997. Immunobiology of the mammary gland. Journal of Dairy Science 80 (8): 1851–65. DOI: https://doi.org/10.3168/jds.S0022-0302(97)76121-6

Spears J W and Weiss W P. 2008. Role of anti-oxidants and trace minerals in health and immunity of transition dairy cows. Veterinary Journal 176 (1): 70–76. DOI: https://doi.org/10.1016/j.tvjl.2007.12.015

Suttle N F and Jones D G. 1989. Recent developments in trace element metabolism and function: Trace elements, disease resistance and immune responsiveness in ruminants. Journal of Nutrition 119: 1055. DOI: https://doi.org/10.1093/jn/119.7.1055

Torre P M, Harmon R J, Hemken R W, Clark T W, Trammell D S and Smith B A. 1996. Mild dietary copper insufficiency depresses blood neutrophil function in dairy cattle. Journal of Nutrition and Immunology 4: 3.

Weiss W P and Spears J W. 2006. Vitamin and trace mineral effects on immune function of ruminants. Ruminant Physiology. pp. 473–96. (Eds) Sejrsen K, Hvelplund T, Nielsen M O Wageningen Academic Publishers, Utrecht, The Netherlands. DOI: https://doi.org/10.3920/9789086865666_019

Wheelock J V, Rook J A F, Neave F K and Dodd F H. 1996. The effect of bacterial infection of the udder on the yield and composition of cow’s milk. Journal of Dairy Research 33: 99–215. DOI: https://doi.org/10.1017/S0022029900011869

Xin Z, Hemken R W, Waterman D F and Harmon R J. 1991. Effects of copper status on neutrophil function, superoxide dismutase, and copper distribution in steers. Journal of Dairy Science 74: 3078. DOI: https://doi.org/10.3168/jds.S0022-0302(91)78493-2