Endocrinological interventions and subsequent conception of repeat breeding cows having cystic ovarian follicles

Abstract

The experiment was intended to evaluate the efficacy of various hormonal interventions for treating cystic ovarian follicles and subsequent conception in repeat breeding crossbred cows. Repeat breeding cows (22) with the history of frequent/constant estruses were subjected to rectal palpation of ovaries and blood samplings for plasma progesterone (P4) profiles on day 0 (estrus), 5, 10, 15 and 20 of estrous cycle. The hormonal treatment was given to the cows on the basis of presence/absence of CL and P4 profiles and subsequently divided into group 1 (n=5; control untreated), group 2 (n=8; GnRH alone treated) and group 3 (n=9; GnRH+ PGF2 ex + GnRH treated). Group 1 cows were not treated and advised for breeding at next estrus. Group 2 animals had follicular cyst without CL (P4 = <0.5 ng/ml up to day 5 of estrous cycle) and were administered with 20 flg buserelin acetate intramuscularly at the time of breeding during next estrus. Group 3 cows had f,?llicular cyst along with CL on either of the ovaries (P4= < 1 ng/ml up to day 10 of estrous cycle) were given 20 Ilg buserelin acetate intramuscularly at spontaneous estrus (day 0) followed by 25 Jlg dinoprost intramuscularly at 12 th day of estrous cycle followed by breeding at induced estrus along with administration of 20 Jlg buserelin acetate intramuscularly. The pregnancy diagnosis was done at 90 days post-breeding. Amongst control group (group 1), no cow was found pregnant. In group 2, 3/8 (37.5%) cows whereas, in group 3, 5/9 (55.5%) cows were pregnant. Hence it was concluded that GnRH+ PGF2 a + GnRH was more effective than GnRH alone to intervene repeat breeding due to cystic ovarian follicle.