Evaluation of comparison of recombinant Omp 28 antigen in indirect ELISA for sero-prevalence study of canine brucellosis
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Authors
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C MATHUR
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D K SINHA
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P CHOUDHURY
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HARI SHANKAR
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S K SRIVASTAVA
Keywords:
Canine brucellosis, Indirect ELISA, Recombinant Outer membrane protein 28 (rec. Omp 28), Sonicated antigenAbstract
Brucella melitensis ree. Omp 28 was evaluated and compared as antigen in I-ELISA with HSE and SA as well as with RBPT. Dog serum samples (787) were collected and tested. Asero-prevalence of 12.3, 8.3, 7.6 and 4.3% of canine brucellosis was recorded by ree. Omp ELISA, SA ELISA, RBPT, HSE ELISA, respectively. Significant differences were observed between sera-prevalence rates estimated by all tests except between SAELISA and RBPT. The ree. Omp ELISA had the highest relative sensitivity while HSE ELISA exhibited the maximum relative specificity in comparison with RBPT. The present study advocated to use rec. Omp 28 antigen based ELISA for diagnosis of canine brucellosis.
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