Polymorphism of keratin-associated protein (KAP) 3.2 gene and its association with wool traits in Rambouillet sheep

VIKAS MAHAJAN; A K DAS; R K TAGGAR; DHIRENDER KUMAR; N KUMAR

doi:10.56093/ijans.v85i3.47313

Authors

VIKAS MAHAJAN Assistant Professor, ILFC, KCVAS, Amritsar
A K DAS Principal Scientist, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
R K TAGGAR Associate Professor and Head, Sher-e-Kashmir University of Agricultural Science and Technology-Jammu, R S Pura, Jammu and Kashmir 181 102 India
DHIRENDER KUMAR Assistant Professor, Division of Animal Genetics and Breeding, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Science and Technology-Jammu, R S Pura, Jammu and Kashmir 181 102 India
N KUMAR Assistant Professor, Division of Animal Genetics and Breeding, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Science and Technology-Jammu, R S Pura, Jammu and Kashmir 181 102 India

https://doi.org/10.56093/ijans.v85i3.47313

Keywords:

Keratin associated protein 3.2, PCR-SSCP, Polymorphisms, Sheep, Wool traits

Abstract

In the present study, association of polymorphic variants of keratin-associated protein (KAP) 3.2 gene with wool traits in Rambouillet sheep was investigated. Genomic DNA was isolated from blood samples of 100 Rambouillet sheep. A 393 bp segment was amplified by PCR using ovine specific primers for KAP 3.2 gene. The genotyping was done by using PCR-SSCP. KAP 3.2 gene locus revealed 3 genotypes, viz. AA, AB and BB with genotype frequencies 0.46 (46), 0.40 (40) and 0.14 (14), respectively, with allele frequencies for allele A (0.66) and allele B (0.34). The result showed that the population was in Hardy-Weinberg equilibrium. Population genetic indexes such as expected heterozygosity (He) 0.45, effective number of alleles (ne) 1.81, Shannon indices (I) 0.64, Fixation index of individual with sub-population (FIS) 0.11, Standard error (SE) 0.03 and polymorphic information content (PIC) 35% were found. The least squares means of various genotypes for greasy fleece weight (GFW) differed significantly. The animals with AB genotype (2.08±0.08 kg) had higher GFW followed by AA genotype (1.75±0.08 kg), and BB genotypes (1.71Â±0.15 kg) was found to be associated. From the study, it may be concluded that KAP 3.2 gene might be a potential molecular marker for genetic selection in Rambouillet sheep.

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References

Barba C, Mendez S, Martí M, Parra J L and Coderch L. 2009. Water content of hair and nails. Thermochimica Acta 494: 136– 40. DOI: https://doi.org/10.1016/j.tca.2009.05.005

Bassam B J, Caetano-Anolles G and Gresshoff P M. 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry 196: 80–83. DOI: https://doi.org/10.1016/0003-2697(91)90120-I

Botstein D, White R L, Skolnick M and Davis R W. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics 32: 314–31.

Chen H Y, Zeng X C, Hui W Q, Zhao Z S and Jia B. 2011. Developmental expression patterns and association analysis of sheep KAP 8.1 and KAP 1.3 genes in Chinese Merino sheep. Indian Journal of Animal Sciences 81(4): 391–96.

Department of Animal Husbandry Dairying and Fisheries (DAHDAF). 2010. Estimates of Eighteen livestock census. Government of India, New Delhi, India.

Feughelman M. 1996. Mechanical Properties and Structure of Alpha-Keratin Fibres: Wool, Human Hair and Related Fibres. UNSW Press Sydney, Australia. http://www.indiaenvironmentportal.org.in/files/file/J&K%20EconomicSurvey%202013–2014.pdf

Itenge-Mweza T O, Hickford J G H, Forrest R H J, McKenzie G W and Frampton C M. 2009. Improving the quality of wool through the use of gene markers. South African Journal of Animal Science 39(1): 219–23. DOI: https://doi.org/10.4314/sajas.v39i1.61191

McLaren R. J, Rogers G R, Davies K P, Maddox J F and Montgomery G W. 1997. Linkage mapping of wool keratin and keratin-associated protein genes in sheep. Mammalian Genome 8: 938–40. DOI: https://doi.org/10.1007/s003359900616

Plowman J E. 2003. Proteomic database of wool components. The Journal of Chromatography B 787: 63–76. DOI: https://doi.org/10.1016/S1570-0232(02)00211-8

Powell B C. 1996. The keratin proteins and genes of wool and hair. Wool Technology and Sheep Breeding 44: 100–18.

Rogers M A, Langbein L, Praetzel Wunder S, Winter H and Schweizer J. 2006. Human hair keratin associated proteins (KAPs). International Review of Cytology 251: 209–63. DOI: https://doi.org/10.1016/S0074-7696(06)51006-X

Schweizer J, Bowden P E, Coulombe P A, Langbein L, Lane E B, Magin T M, Maltais L, Omary M B, Parry D A, Rogers M A and Wright M W. 2006. New consensus nomenclature for mammalian keratins. Journal of Cell Biology 174: 169–74. DOI: https://doi.org/10.1083/jcb.200603161

Sethi I C. 2006. Statistical Package for Animal Breeding (SPAB2). Project Report, IASRI, New Delhi.

Wang Z Y, Chen Y L, Xu Q L, Ma Z R and Qi Q Q. 2011. Polymorphism of KAP 3.2 gene and its effect on partial economic traits in Tibetan sheep. Acta Veterinaria et Zootechnica Sinica 42(2): 284–88.

Wang Z Y, Qi Q and Chen Y. 2010a. Study of the correlations between polymorphism of KAP gene and wool production traits in plateau Tibetan sheep. Chinese Agricultural Science Bulletin 26(14): 18–22.

Wang Z Y, Qi Q and Chen Y. 2010b. Analysis on single nucleotide polymorphisms of keratin-associated proteins gene in plateau Tibetan sheep. China Animal Husbandry and Veterinary Medicine 37(10): 120–24.

Xu H F, Zhao Z S, Xue A Y, Amina Li G L, Ban Q and Zhang J. 2008. Studies on the relation between KAP 1.3 gene and 5 breeds of sheep, and with some wool traits. Journal of Shihezi University (Natural Science) 26(1): 60–63.

Yeh F C, Yang R C, Boyle B J T, Ye Z H and Mao X J. 1999. POPGENE 32 version 1.32, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada. URL:<http://www.ualberta.ca/~fyeh/fyeh >