Development of 12 polymorphic EST-SSR for endangered fish, Hucho taimen (Pallas)


Keywords:
cDNA library, EST-SSR, Genetic diversity, Hucho taimen (Pallas)Abstract
Hucho taimen (Pallas) is an indigenous and endangered cold-water fish in China. To access the genetic diversity of wild breeding parents of this species and promote its breeding selection program, 12 polymorphic EST-SSR markers were developed from its cDNA library by (CA)16 probe and were analyzed on 66 wild breeding parents. The number of alleles per locus (Na), expected heterozygosity (He) and polymorphism information content (PIC) ranged from 2 to 9, 0.2019 to 0.8542 and 0.1802 to 0.8312 respectively. In total, a new and economical method for EST-SSR was established. The EST-SSR markers characterized in this study will strengthen our interpretation of Hucho taimen and facilitate its breeding selection program to improve the endangered statue of this species.Downloads
Downloads
Submitted
Published
How to Cite
Issue
Section
License
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.