An initial molecular signature of Indian isolates of Toxocara

K JEEVA; N CHATTERJEE; A K BERA; S MAITI; HIRA RAM; P S BANERJEE; T K GOSWAMI; P J DAS; J CHAMUA; A A P MILTON; M ARAVIND; G BHUVANAPRIYA; K THILAGAVATHI; S K GHOSH; S M DEB; D BHATTACHARYA

doi:10.56093/ijans.v85i7.50230

Authors

K JEEVA Principal Scientist, ICAR-National Research Centre on Yak, West Kameng District, Arunachal Pradesh 790 101 India
N CHATTERJEE Principal Scientist, ICAR-National Research Centre on Yak, West Kameng District, Arunachal Pradesh 790 101 India
A K BERA Principal Scientist, ICAR-National Research Centre on Yak, West Kameng District, Arunachal Pradesh 790 101 India
S MAITI Principal Scientist, ICAR-National Research Centre on Yak, West Kameng District, Arunachal Pradesh 790 101 India
HIRA RAM Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
P S BANERJEE Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
T K GOSWAMI Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
P J DAS Principal Scientist, ICAR–National Research Centre on Yak, West Kameng District, Arunachal Pradesh 790 101 India
J CHAMUA National Research Centre on Mithun, Jharnapani, Medziphama, Nagaland
A A P MILTON Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
M ARAVIND Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
G BHUVANAPRIYA Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
K THILAGAVATHI Veterinary College and Research Institute, Namakkal, Tamil Nadu 637 002 India
S K GHOSH Department of Biotechnology, Assam University, Silchar, Asom
S M DEB Principal Scientist, ICAR-National Research Centre on Yak, West Kameng District, Arunachal Pradesh 790 101 India
D BHATTACHARYA Principal Scientist, ICAR–National Research Centre on Yak, West Kameng District, Arunachal Pradesh 790 101 India

https://doi.org/10.56093/ijans.v85i7.50230

Keywords:

Molecular signature, Toxocara canis, Toxocara cati, Toxocara vitulorum

Abstract

This communication deals with molecular signature of Toxocara canis, T. cati and T. vitulorum collected from different geographical locations and host assemblages of India. T. vitulorum was collected from cattle, yak and mithun of West Bengal, Arunachal Pradesh and Nagaland, respectively. Isolated parasites were initially identified morphologically before proceeding for molecular characterization. ATP synthase subunit 6 (atp6) gene had a 598 bp stretch which contained both the punctuation codons but was unique in its characteristics due to presence of abbreviated stop codon (T). On the basis of phylogenetic analysis of atp6, 12S and transcribed spacer sequences, three species could be clustered in three different groups. Number of preferred and non preferred codons also varied in between three species of Toxocara of Indian origin. Atp6 gene had abundance in guanine (G) and thymine (T) bases which has also been described as unique characteristic for Neodermata. Restriction profile of transcribed sequences, 5.8S gene and a small fragment of 28S gene could differentiate Indian isolates of Toxocara in two different clades.

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