Sex differentiation in diversified poultry species by PCR based molecular method from blood

ABDUL RAHIM; SANJEEV KUMAR; LAXMIKANT SAMBHAJI KOKATE; ANANTA KUMAR DAS

doi:10.56093/ijans.v85i7.50302

Authors

ABDUL RAHIM Ph.D. Scholar, Molecular Genetics Laboratory, Central Avian Research Institute, Izatnagar
SANJEEV KUMAR Principal Scientist, Avian Genetics and Breeding, Central Avian Research Institute, Izatnagar
LAXMIKANT SAMBHAJI KOKATE Livestock Development Officer, Department of Animal Husbandry, Karanja, Wardha, Maharasthtra
ANANTA KUMAR DAS Subject Matter Specialist, Howrah KVK, Jagatballavpur, Howrah, West Bengal

https://doi.org/10.56093/ijans.v85i7.50302

Keywords:

Blood, Diversified poultry, PCR, Sex differentiation, W-chromosome

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References

Clinton M. 1994. A rapid protocol for sexing chick embryos (Gallus g. domesticus). Animal Genetics 25: 361–62. DOI: https://doi.org/10.1111/j.1365-2052.1994.tb00374.x

Dubiec A and Zagalska –Neubauer M. 2006. Molecular techniques for sex identification in birds. Biological Letter 43: 3–12.

Ellegren H. 1996. First gene on the avian W chromosome (CHD) provides a tag for universal sexing of non–ratite birds. Proceedings of the Royal Society of London B 263: 1635–41. DOI: https://doi.org/10.1098/rspb.1996.0239

Hofstetter J R, Zhang A, Mayeda A R, Guscar T, Nurnberger J J I and Lahiri D K.1997. Genomic DNA from mice: A comparison of recovery methods and tissue sources. Biochemical and Molecular Medicine 62: 197–202. DOI: https://doi.org/10.1006/bmme.1997.2637

Kagami H, Nakamura H and Tomita T. 1990. Sex identification in chickens by means of the presence of the W chromosomespecific repetitive DNA units. Japanese Poultry Science 27: 379–84. DOI: https://doi.org/10.2141/jpsa.27.379

Khatib H and Gruenbaum Y. 1996. Chicken red blood cells as a substrate for direct polymerase chain reaction. Animal Genetics 27: 53–54. DOI: https://doi.org/10.1111/j.1365-2052.1996.tb01177.x

Mizuno S and Macgregor H. 1998. The ZW lampbrush chromosomes of birds: a unique opportunity to look at the molecular cytogenetics of sex chromosomes. Cytogenetic and Cell Genetics 80: 149–57. DOI: https://doi.org/10.1159/000014972

Morinha F, Cabral J A and Bastos E. 2012. Molecular sexing of birds: A comparative review of polymerase chain reaction (PCR) based method. Theriogenology 78: 703–14. DOI: https://doi.org/10.1016/j.theriogenology.2012.04.015

Nakabayashi O, Kikuchi H, Kikuchi T and Mizuno S 1998. Differential expression of genes for aromatase and estrogen receptor during the gonadal development in chicken embryos. Journal of Molecular Endocrinology 20: 193–202. DOI: https://doi.org/10.1677/jme.0.0200193

Ogawa A, Solovei I, Hutchison N, Saitoh Y, Ikeda J E, Macgregor H and Mizuno S. 1997. Molecular characterization and cytological mapping of a non-repetitive DNA sequence region from the W chromosome of chicken and its use as a universal probe for sexing Carinatae birds. Chromosome Research, 5: 93–101. DOI: https://doi.org/10.1023/A:1018461906913

Ogawa A, Murata K and Mizuno S. 1998.The location of Z- and W-linked marker genes and sequence on the homomorphic sex chromosomes of the ostrich and the emu. Proceeding of Natural Academy Science, 95: 4415–18. DOI: https://doi.org/10.1073/pnas.95.8.4415

Rahim A, Kumar S, Sharma D, Kokate L S, Das A K, Singh J, and Kataria M C. 2012. Species and gender identification in diversified poultry species by PCR analysis of CHD gene. Indian Journal of Poultry Science 47: 281–86.

Tomasulo A M, Silvia N D L and Rocha C D. 2002. Molecular method of Sexing Waterbirds without DNA Extraction. International Journal of Waterbird Biology 25: 245– 48. DOI: https://doi.org/10.1675/1524-4695(2002)025[0245:MMOSWW]2.0.CO;2