Laparoscopy Liver biopsy through cauterization in dogs

Abstract

Liver biopsy was collected through laparoscopy with simultaneous cauterization in the present study, whose principal airn was to evaluate the efficacy of the applied nlethod and to study its effects in the liver of healthy dogs. Furthermore, we tried to verify the main haematological and biochemical profile alterations related to the liver function, and to investigate the viability of the fragments collected by histopathology. To attain this objective, 18 clinically healthy dogs were submitted to hepatic biopsy with forceps connected to the cautery, applying frequency energy at 45 watts performed cautery. Thirtysix liver biopsies through laparoscopy were conducted in the animals. In group 1, one hepatic fragment per animal was collected, in group 2, two hepatic fragments per animal were collected and in group 3, thrc:e fragments per animals were collected. Haematocrit and alanine-mninotransferase measurements were conducted to evaluate the animals at the pre biopsy period, at 1and 2 h and day at 1, 3 and 7 post biopsy. The results revealed that the procedure was safe and effective for liver biopsy in dogs. There were no clinical and haemato-biochenlical alterations related to the technique. The fragments collected were viable for histopathology. Liver biopsy through laparoscopy with simultaneous cauterization was effective and useful in dogs. This technique was successfully applied in other small species of animals.