PCR detection of Pasteurella multocida in eagle PCR based detection, characterization and antibiogram of Pasteurella multocida from wild eagle (Aquila rapax) in Jammu and Kashmir

Abstract

Pasteurella multocida, a gram negative, coccobacilli was isolated and identified from a dead wild eagle in Jammu and Kashmir. Cultural and biochemical tests proved the association of P. multocida. Species ,and type specific multiplex PCR assay confirmed the presence of P. multocida other than serotype B:21B:2,51B:5. Isolate was recorded as a multi-drug resistant P. multocida by antimicrobial sensitivity assay using disc diffusion method. This is probably first report to isolate a multi-drug resistant P. multocida from a wild eagle in India.