Storage stability of certain serum enzymes of chicken (Gallus domesticus)

Abstract

Many times loss of activity of some of the diagnostically important enzymes before estimation would lead to false interpretation of the result in veterinary practice. The present study was designed to assess the effect of time and various storage temperatures on the activity of chicken serum enzylne like creatine kinase (CK), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), choline esterase (ChE), alanine amino transferase(ALT) and aspartate amino transferase (AST). It was observed that at the end of 10 days of post collection, the activity of ALT and ChE was significantly (P<0.05) higher in the serum samples stored at freezing (-200 C) temperature when cOlnpared to sample stored at 40 C and 37° C. Sera stored at 40 C showed significantly (P<0.05) higher values for the activity of ALP, LDH and AST, while comparing with other temperature considered for storage on day '10' post collection. It was also found that among these six enzymes tested CK was more thermo labile.