A new cell line from goat embryonic kidney may be a candidate to produce influenza virus vaccine


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Authors

  • MIN XIANG Department of Animal Nutrition and Reproductio, Wuhan Institute of Animal and Veterinary Science, Wuhan, Hubei, PR 430 065 China
  • SHUN LU Department of Animal Nutrition and Reproduction, Wuhan Institute of Animal and Veterinary Science, Wuhan, Hubei, PR 430 065 China
  • QISHUANG GAO Department of Animal Nutrition and Reproduction, Wuhan Institute of Animal and Veterinary Science, Wuhan, Hubei, PR 430 065 China
  • HAIJUN HUANG Department of Animal Nutrition and Reproduction, Wuhan Institute of Animal and Veterinary Science, Wuhan, Hubei, PR 430 065 China
  • ZHIHUA CHEN Department of Animal Nutrition and Reproduction, Wuhan Institute of Animal and Veterinary Science, Wuhan, Hubei, PR 430 065 China
  • BIFEI TAO Department of Animal Nutrition and Reproduction, Wuhan Institute of Animal and Veterinary Science, Wuhan, Hubei, PR 430 065 China
  • CAIYAO ZHAN Department of Animal Nutrition and Reproduction, Wuhan Institute of Animal and Veterinary Science, Wuhan, Hubei, PR 430 065 China
  • YUNGUO QIAN Department of Animal Nutrition and Reproduction, Wuhan Institute of Animal and Veterinary Science, Wuhan, Hubei, PR 430 065 China
  • RENLI SUN Department of Animal Nutrition and Reproduction, Wuhan Institute of Animal and Veterinary Science, Wuhan, Hubei, PR 430 065 China
  • YU XIA Department of Animal Nutrition and Reproduction, Wuhan Institute of Animal and Veterinary Science, Wuhan, Hubei, PR 430 065 China
  • WEIWEN TONG Department of Animal Nutrition and Reproduction, Wuhan Institute of Animal and Veterinary Science, Wuhan, Hubei, PR 430 065 China
  • LIANFANG WANG Department of Animal Nutrition and Reproduction, Wuhan Institute of Animal and Veterinary Science, Wuhan, Hubei, PR 430 065 China
  • JUAN HUA Department of Animal Nutrition and Reproduction, Wuhan Institute of Animal and Veterinary Science, Wuhan, Hubei, PR 430 065 China
  • LIWEN TAO Department of Animal Nutrition and Reproduction, Wuhan Institute of Animal and Veterinary Science, Wuhan, Hubei, PR 430 065 China

https://doi.org/10.56093/ijans.v85i8.50930

Keywords:

Goat embryonic kidney cell, Influenza virus, Receptor, Tumorigenicity

Abstract

This study established a goat embryonic kidney (GEK) cell line which was susceptible to influenza virus. Kidney cells were separated from fetus of Matou goat and GEK cell line was established by cell biology techniques. Tumorigenicity of GEK cell line was tested by tumorigenesis assay, and its marker protein expression was tested by immunohistochemistry. Receptors of influenza virus and susceptibility to H1N1 influenza virus were also tested by immunofluorescence method and cell infection assay, respectively. Results showed that GEK cells transplanted into BALB/c nude mice could not induce tumorigenesis. Immunohistochemical analysis showed that the cells expressed cytokeratin 18 (CK-18) and vimentin which are marker proteins of renal epithelial cells. Immunofluorescent assay indicated that the abundance of α 2,3-sialic acid (SA) receptor and α 2,6-SA receptor on the surface of GEK cells were higher than that of Madin-Darby canine kidney (MDCK) cells. The influenza virus titers in GEK cells were similar to that of MDCK cells. Therefore, the GEK cell line established here will possibly contribute to the future influenza virus isolation and vaccines development.

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Submitted

2015-08-10

Published

2015-08-10

Issue

Vol. 85 No. 8 (2015)

Section

Articles

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Copyright (c) 2015 The Indian Journal of Animal Sciences

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The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.

How to Cite

XIANG, M., LU, S., GAO, Q., HUANG, H., CHEN, Z., TAO, B., ZHAN, C., QIAN, Y., SUN, R., XIA, Y., TONG, W., WANG, L., HUA, J., & TAO, L. (2015). A new cell line from goat embryonic kidney may be a candidate to produce influenza virus vaccine. The Indian Journal of Animal Sciences, 85(8), 828–831. https://doi.org/10.56093/ijans.v85i8.50930
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