Detection of Arcobacter spp in poultry, pigs, their meat and environment samples by conventional and PCR assays

MOHIT VERMA; NAMITA JOSHI; R S RATHORE; H V MOHAN

doi:10.56093/ijans.v85i9.51694

Authors

MOHIT VERMA Department of Veterinary Public Health, College of Veterinary Science, Narendra Dev University of Agriculture and Technology, Kumarganj, Uttar Pradesh 224 229 India
NAMITA JOSHI Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
R S RATHORE Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
H V MOHAN Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v85i9.51694

Keywords:

Arcobacter, Characterization, Environment, Pig, Poultry

Abstract

Samples (300) comprising poultry and pig faeces, meat, poultry intestinal contents and environmental samples were investigated bacteriologically for the presence of Arcobacter spp. On the basis of morphology and biochemical tests, 34 (11.33%) of the isolates were identified as Arcobacter. The isolates grew at 28°C aerobically but failed to grow at 42°C. Arcobacters were differentiated from closely related campylobacters by their ability to grow in aerobic condition and negative for hippurate hydrolysis test. The genus specific amplification of 16S rRNA gene by PCR gave an amplification product of 1223 bp in all 34 presumptive Arcobacter isolates. The highest rate of Arcobacter isolation was from poultry meat samples (18%) followed by poultry environmental samples (16%), poultry intestinal contents (13.33%), poultry faecal (10%), pork (10%) and pig faecal (8%) and no arcobacters could be isolated from the pig environments. Multiplex PCR (m-PCR) targeting for 16S r RNA and 23S rRNA genes detected A. butzleri (12), A. skirrowii (6) and A. cryaerophilus (4). However, some of isolates showed mixed culture of both A. butzleri and A. skkirrowii (5), A. skkirrowii and A. cryaerophilus (4) and A. butzleri and A. cryaerophilus (3).

Downloads

Download data is not yet available.

References

Amare L B, Saleha A A, Zunita Z, Jalila A and Hassan L. 2011. Prevalence of Arcobacter spp. on chicken meat at retail markets and in farm chickens in Selangor, Malaysia. Food Control 22: 732–36. DOI: https://doi.org/10.1016/j.foodcont.2010.11.004

Collado L, Cleenwerck I, Trappen S V, Vos P D and Figueras M J. 2009. Arcobacter mytili sp. nov. isolated from Mussels in Spain. International Journal of Systematic and Evolutionary Microbiology 59: 1391–96. DOI: https://doi.org/10.1099/ijs.0.003749-0

Harrab B, Schwarz S and Wenzel S. 1998. Identification and characterization of Arcobacter isolates from broilers by biochemical tests, antimicrobial resistance patterns and plasmid analysis. Journal of Veterinary Medicine 45: 87–94. DOI: https://doi.org/10.1111/j.1439-0450.1998.tb00770.x

Harmon K M and Wesley I V. 1996. Identification of Arcobacter isolates by PCR. Letters Applied Microbiology 23: 241–44. DOI: https://doi.org/10.1111/j.1472-765X.1996.tb00074.x

Ho H T, Lipman L J and Gaastra W. 2006. Arcobacter, what is known and unknown about a potential foodborne zoonotic agent. Veterinary Microbiology 115: 1–13. DOI: https://doi.org/10.1016/j.vetmic.2006.03.004

Ho T K H, Lipman L J A and Gaastra W. 2008. The introduction of Arcobacter spp. poultry slaughterhouses. International Journal of Food Microbioogy 125: 223–29. DOI: https://doi.org/10.1016/j.ijfoodmicro.2008.02.012

Houf K, De Zutter L, Van Hoof J and Vandamme P. 2003. Molecular characterization of Arcobacter isolates collected in a poultry slaughter house. Journal of Food Protection 66: 364–69. DOI: https://doi.org/10.4315/0362-028X-66.3.364

Houf K, On S L, Coenye T, Mast J, Van Hoof J, Vandamme P 2005. Arcobacter cibariussp. nov., isolated from broiler carcasses. International Journal of Systematic Evolutionary Microbiology 55(2): 713–17. DOI: https://doi.org/10.1099/ijs.0.63103-0

Houf K, On S L, Coenye T, Debruyne L, De Smet and S, Vandamme P. 2009. Arcobacter thereius sp. nov., isolated from pigs and ducks. International Journal of Systematic Evolutionary Microbiology 59: 2599–604. DOI: https://doi.org/10.1099/ijs.0.006650-0

Jacob J, Loir H and Feuerpfeil I. 1993. Isolation of Arcobacter butzleri from drinking water reservoir in Eastern Germany. Zentralblatt-fuer-Hygiene193: 557–62.

Jiang Z, DuPont, H L, Brown E L, Nandy R K, Ramamurthy T, Sinha A, Ghosh S, Guin S, Gurleen K, Rodrigues S, Chen J J, McKenzie R and Steffen R. 2010. Microbial etiology of travelers’ diarrhea in Mexico, Guatemala, and India: importance of enterotoxigenic Bacteroides fragilis and Arcobacter species. Journal of Clinical Microbiology 48(4): 1417–19. DOI: https://doi.org/10.1128/JCM.01709-09

Kabeya H, Maruyama S, Morita Y, Kubo M, Yamamoto K, Arai S, Izumi T, Kobayashi Y, Katsube Yand Mikami T. 2003. Distribution of Arcobacter species among livestock in Japan. Veterinary Microbiology 93: 153–58. DOI: https://doi.org/10.1016/S0378-1135(02)00312-7

Kabeya, H., Maruyama, S., Morita, Y., Ohsuga, T., Ozawa, S., Kobayashi, Y., Abe, M., Katsube, Y. and Mikami, T. 2004. Prevalence of Arcobacter species in retail meats and antimicrobial susceptibility of the isolates in Japan. International Journal of Food Microbiology 90: 303–08. DOI: https://doi.org/10.1016/S0168-1605(03)00322-2

Lehner A, Tasara T, Stephan R. 2005. Relevant aspects of Arcobacter spp. as potential food-borne pathogens. International Journal of Food Microbiology 102: 127–35. DOI: https://doi.org/10.1016/j.ijfoodmicro.2005.03.003

Mohan H V. 2008. ‘Detection of Arcobacter spp. in man, animals and foods of animal origin by using cultural and PCR techniques.’ M.V.Sc Thesis, Deemed University, IVRI, Izatnagar.

Patyal A, Rathore R S, Mohan H V, Dhama K and Kumar A. 2011. Prevalence of Arcobacter spp. in humans, animals and foods of animal origin including sea food from India. Transboundary and Emerging Diseases 58 (5): 402–10. DOI: https://doi.org/10.1111/j.1865-1682.2011.01221.x

Prouzet-Mauleon V, Labadi L, Bouges N, Menard A and Megraud F. 2006. Arcobacter butzleri: underestimated enteropathogen. Emerging Infectious Diseases 12: 307–09. DOI: https://doi.org/10.3201/eid1202.050570

Van Driessche E, Houf K, Vangroen weghe F, De Zutter L and Van Hoof J. 2003. Prevalence, enumeration and strain variation of Arcobacter species in the faeces of healthy cattle in Belgium. Veterinary Microbiology. 105(2): 149–54. DOI: https://doi.org/10.1016/j.vetmic.2004.11.002

Vandamme P and De Ley J.1991. Proposal for a new family, Campylobacteraceae. International Journal of Systematic Bacteriology 41: 451–55. DOI: https://doi.org/10.1099/00207713-41-3-451

Vandamme P, Vancanneyt M, Pot B, Mels L, Hoste B, Dewettinck D, Vlaes L, van den Borre C, Higgins R and Hommez J. 1992. Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleria comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. International Journal of Systemtic Bacteriology 42(3): 344–56. DOI: https://doi.org/10.1099/00207713-42-3-344