Novel PCR-RFLP for detecting single nucleotide polymorphism in exon1 of GPR54 gene and its association with age at first kidding in goats*


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Authors

  • G RADHIKA Assistant Professor, Department of Animal Genetics and Breeding, Kerala Veterinary and Animal Sciences University, Wayanand, Kerala 673 121 India
  • K C RAGHAVAN Professor, Kerala Veterinary and Animal Sciences University, Wayanand, Kerala 673 121 India
  • T STEPHY Research Associate, Kerala Veterinary and Animal Sciences University, Wayanand, Kerala 673 121 India
  • T V ARAVINDAKSHAN Professor, Kerala Veterinary and Animal Sciences University, Wayanand, Kerala 673 121 India
  • K A MERCEY Professor, Department of Statistics, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Wayanand, Kerala 673 121 India
  • T VENKATACHALAPATHY Associate Professor, CASAGB, Mannuthy

https://doi.org/10.56093/ijans.v85i9.51760

Keywords:

Age at first kidding, Goats, GPR54 gene, KiSS peptides, Litter size, PCR-RFLP

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References

Anonymous. 2004. Project completion report of ICAR ADHOC project on Conservation and Evaluation of Malabari goats. COVAS, Mannuthy.

Aparcio S A J R. 2005. Kisspeptins and GPR54- The new biology of the mammalian GnRH axis. Cell Metabolism 1: 293–96. DOI: https://doi.org/10.1016/j.cmet.2005.04.001

Cao G L, Chu M X, Fang L, Di R, Feng T and Li N. 2010. Analysis on DNA sequence of Kiss-1 gene and its association with litter size in goats. Molecular Biology Reports 37: 3921–29. DOI: https://doi.org/10.1007/s11033-010-0049-7

Colledge W H. 2004. GPR54 and puberty. Trends in Endocrinology and Metabolism 15: 9448–53. DOI: https://doi.org/10.1016/S1043-2760(04)00218-8

Feng T, Zhao Y Z, Chu M X, Zhang Y J, Fang L, Di R, Cao G L and Li N. 2010. Association between sexual precocity and alleles of KISS-1 and GPR54 genes in goats. Animal Biotechnology 20: 3172–76. DOI: https://doi.org/10.1080/10495390903004493

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Maitra A, Sharma R, Ahlawat S and Tantia M S. 2014a. Novel genetic polymorphisms in Caprine GPR54 gene associated with reproductive functions. Indian Journal of Animal Sciences 84(11): 1196–201.

Maitra A, Sharma R, Ahlawat S and Tantia M S, Roy M and Prakash V. 2014b. Association analysis of polymorphisms in caprine KiSS1 gene with reproductive traits. Animal Reproduction Science 151: 71–77. DOI: https://doi.org/10.1016/j.anireprosci.2014.09.013

Popa S M, Clifton D K and Steiner R A. A KiSS to remember. 2005. TRENDS in Endocinology and Metabolism 16(6): 249–50. DOI: https://doi.org/10.1016/j.tem.2005.06.005

Sambrook J, Fritsch E F and Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. 6.4-6.11. Cold spring Harbor Laboratory Press, New York.

Seminara S B, Messager S, Chatzidaki E E, Thresher R R, Acierno J S, Shagoury J K, Bo-Abbas Y, Kuohong W, Schwinof K M, Hendrick A G, Zahn D, Dixon J, Kaiser U B, Slaugenhaupt S A, Gusella J F, Rahilly S, Carlton M B L, Crowley W F, Aparcio S A J R and Colledge W H. 2003. The GPR54 gene as a regulator of puberty. New England Journal of Medicine 349: 1614–27. DOI: https://doi.org/10.1056/NEJMoa035322

Stephen M, Raja T V and Sosamma I. 2005. Survey and characterisation of Attappady Black goats of Kerala, India. Animal Genetic Resources Information 37: 43–51. DOI: https://doi.org/10.1017/S1014233900001954

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Submitted

2015-09-11

Published

2015-09-11

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Section

Short-Communication

How to Cite

RADHIKA, G., RAGHAVAN, K. C., STEPHY, T., ARAVINDAKSHAN, T. V., MERCEY, K. A., & VENKATACHALAPATHY, T. (2015). Novel PCR-RFLP for detecting single nucleotide polymorphism in exon1 of GPR54 gene and its association with age at first kidding in goats*. The Indian Journal of Animal Sciences, 85(9), 1056–1058. https://doi.org/10.56093/ijans.v85i9.51760
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