Antimicrobial assay of shigatoxigenic E. coli (STEC) and enteropathogenic E. coli (EPEC) isolated from diarrhoeic faecal samples of piglets and infants in Mizoram

Abstract views: 149 / PDF downloads: 17


  • JUBEDA BEGUM PhD, Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
  • TAPAN KUMAR DUTTA Associate Professor, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
  • PARIMAL ROY CHOUDHARY Assistant Professor, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
  • RAJESH CHANDRA PhD, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
  • ZOMUANKIMA VARTE MVSc, Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Selesih


Antimicrobial sensitivity, Enteropathogenic Escherichia coli, Infant diarrhoea, Piglet diarrhoea, Shigatoxigenic Escherichia coli


Antimicrobial resistance is a common problem which is accelerating day by day in veterinary medicine. The main reason is believed to be the indiscriminate and irrational use of antibiotics. Diarrhoeic faecal samples (584: 320 from piglets and 264 from infants) were collected from different farms and hospitals located in different districts of Mizoram. Out of 1,260 E. coli isolates, 65 (5.15%) E. coli isolates were found positive for at least 1 virulence gene (stx1, stx2, eaeA, hlyA) under the study, of which 40 (3.17%) and 25 (1.98%) were recorded as STEC and EPEC, respectively, when screened by multiplex PCR. All the 65 E. coli isolates were subjected to antimicrobial sensitivity test against 12 commonly used antimicrobial agents. Among the isolates from piglets, highest sensitivity was exhibited by chloramphenicol (89.58%) and highest resistance by nalidixic acid (85.41%). On the other hand, enrofloxacin exhibited 100% sensitivity while amoxicillin, polymyxin B and kanamycin exhibited 100% resistance among the infant isolates. It may be concluded that 5.15% isolates were positive for virulence and the isolates showed increased tendency of resistance to many of the commonly used antibiotics reflecting a greater threat to treat the commonly occurring diseases with antibiotics.


Download data is not yet available.


Abea C M, Trabulsia L M, Blancob J, Blancob M, Dahbib G, Blancob J E, Morab A, Franzolina M A, Taddeia C R, Martinezc M B, Piazzaa R M F and Eliasa W P. 2009. Virulence features of atypical enteropathogenic Escherichia coli identified by the eae+ EAF-negative stx” genetic profile. Diagnostic Microbiology and Infectious Disease 64: 357–65. DOI:

Asadi Karam M, Bouzari S, Oloomi M, Aslani M and Jafari A. 2010. Phenotypic and genotypic characterization of nteropathogenic Escherichia coli (EPEC) strains in Tehran, Iran. Iranian Journal of Microbiology 2: 3–7.

Bauer A W, Kirby M M, Sheris J S and Turek M. 1966. Antibiotic sensitivity testing by single disc method. American Journal of Clinical Pathology 45: 493–96. DOI:

Begum J, Dutta T K, Chandra R, Choudhary P R, Varte Z and Bitew M. 2014. Molecular and phenotypic characterization of shigatoxigenic Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) from piglets and infants associated with diarrhoea in Mizoram, India. African Journal of Biotechnology 13: 1452–61. DOI:

Bhat M A, Nishikawa Y and Wani S A. 2008. Prevalence and virulence gene profiles of Shigatoxin- producing Escherichia coli and enteropathogenic Escherichia coli from diarrhoeic and healthy lambs in India. Small Ruminant Research 75: 65– 70. DOI:

Dhanashree B and Mallya P. 2008. Detection of shiga-toxigenic Escherichia coli (STEC) in diarrhoeagenic stool and meat samples in Mangalore, India. Indian Journal of Medical Research 128: 271–77.

Duda-Madej A, Gooeciniak G, Andrzejewska B, Duda A K and Sobieszczañska B. 2013. Association of untypeable enteropathogenic Escherichia coli (EPEC) strains with persistent diarrhea in children from the region of lower Silesia in Poland. Polish Journal of Microbiology 62: 461–64. DOI:

Ghosh P K and Ali A. 2010. Isolation of atypical enteropathogenic Escherichia coli from children with and without diarrhoea in Delhi and the National Capital Region, India. Journal of Medical Microbiology 59: 1156–62. DOI:

Habrun B, Kompes G, Cvetniæ Z, Spicic S, Benic M and Mitak M. 2010. Antimicrobial sensitivity of Escherichia coli, Salmonella spp., Pasteurella multocida, Streptococcus suis and Actinobacillus pleuropneumoniae isolated from diagnostic samples from large pig breeding farms in Croatia. Veterinarski Arhiv 80: 571–83.

Herman V, Pascu C, Costinar L, Faur B, Vãduva I, Surpat A, Irimie S and ªerbescu M E. 2010. Coli strains‘ characterization isolated from pig septicemic colibacilosis. Lucrãri Stiinlifice Medicinã Veterinarã, Timisoara. xliii (1): 93–96.

Iseri L, Zafer Apan T, Aksoy A, Koç F, Sedef Göçmen J and Nuristani D. 2011. The prevalence of Enterotoxigenic E. coli Isolated from the stools of children aged 0–10 years with diarrhea in Mid-Anatolia Region, Turkey. Brazilian Journal of Microbiology 42: 243–47. DOI:

Kikuvi G M, Ole-Mapenay I, M, Mitema E S and Ombui J N. 2013. Antimicrobial resistance in Escherichia coli isolates from faeces and carcass samples of slaughtered cattle, swine and chickens in Kenya.

Laxminarayan R, Duse A, Wattal C, Zaidi A K, Wertheim H F, Sumpradit N, Vlieghe E, Hara G L, Gould I M, Goossens H, Greko C, So A D, Bigdeli M, Tomson G, Woodhouse W, Ombaka E, Peralta A Q, Qamar F N, Mir F, Kariuki S, Bhutta Z A, Coates A, Bergstrom R, Wright G D, Brown E D and Cars O. 2013. Antibiotic resistance-the need for global solutions. Lancet Infectious Diseases 13: 1057–98. DOI:

Mandakini R, Dutta T K, Chingtham S, Roychoudhury P, Samanta I, Joardar S N, Pachauau A R and Chandra R. 2015. ESBLproducing Shiga-toxigenic E. coli (STEC) associated with piglet diarrhoea in India. Tropical Animal Health and Production 47: 377–81. DOI:

Mekata H, Iguchi A, Kawano K, Kirino Y, Kobayashi I and Misawa N. 2014. Identification of O serotypes, genotypes, and virulotypes of Shiga toxin-producing Escherichia coli isolates, including non-O157 from beef cattle in Japan. Journal of Food Protection 77: 1269–74. DOI:

Meng Q, Bai X, Zhao A, Lan R, Du H, Wang T, Shi C, Yuan X, Bai X, Ji S, Jin D, Yu B, Wang Y, Sun H, Liu K, Xu J and Xiong Y. 2014. Characterization of Shiga toxin-producing Escherichia coli isolated from healthy pigs in China. BMC Microbiology 14: 5. doi: 10.1186/1471–2180–14–5 DOI:

Moyo S J, Maselle S Y, Matee M I, Langeland N and Mylvaganam H. 2007. Identification of diarrheagenic Escherichia coli isolated from infants and children in Dar el Salaam, Tanzania. BMC Infectious Diseases 7: 1–7. DOI:

Nataro J P and Kaper J B. 1998. Diarrhoeagenic Escherichia coli. Clinical Microbiology Reviews 11: 142–201. DOI:

Paton J C and Paton A W. 1998. Pathogenesis and diagnosis of Shiga toxin-producing E. coli infections. Clinical Microbiology Reviews 11: 450–79. DOI:

Pennington, H. 2010. Escherichia coli O157. Lancet 376: 1428– 35. DOI:

Rajii M A, Minga U M and Machang R S. 2008. Prevalence and characterization of verotocytoxin producing Escherichia coli O157 from diarrhoea patients in Morogoro, Tanzania. Tanzania Journal of Health Research 10: 151–58. DOI:

Rivero M A, Passucci J A, Rodriguez E M and Parma A E. 2010. Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina. Journal of Medical Microbiology 59: 345–52. DOI:

Rodas C, Mamani R, Blanco J, Blanco J E, Wiklund G, Svennerholm A, Sjoling A and Iniguez V. 2011. Enterotoxins, colonization factors, serotypes and antimicrobial resistance of enterotoxigenic Escherichia coli (ETEC) strains isolated from hospitalized children with diarrhea in Bolivia. Brazilian Journal of Infectious Diseases 15: 132–37. DOI:

Schroeder C M, Zhao C, DebRoy C, Torcolini J, Zhao S, White D G, Wagner D D, McDermott P F, Walker R D and Meng J. 2002. Antimicrobial resistance of Escherichia coli O157 isolated from humans, cattle, swine, and food. Applied and Environmental Microbiology 68: 576–81. DOI:

Shakya P, Barrett P, Diwan V, Marothi Y, Shah H, Chhari N, Tamhankar A J, Pathak and, Lundborg C S. 2013. Antibiotic resistance among Escherichia coli isolates from stool samples of children aged 3 to 14 years from Ujjain, India. BMC Infectious Diseases 13: 477. doi: 10.1186/1471–2334– 13–477. DOI:

Sudershan R V, Naveen Kumar R, Kulkarni Bharathi, Kashinath L, Bhaskar V and Polasa K. 2014. E. coli pathotypes and their antibiotic resistance in young children with diarrhea in Hyderabad, India. International Journal of Current Microbiology and Applied Science 3(9): 647–54. DOI:

Tadesse D A, Zhao S, Tong E, Ayers S, Singh A, Bartholomew M J and McDermott P F. 2012. Antimicrobial drug resistance in Escherichia coli from humans and food animals. Emerging Infectious Diseases 18: 741–49. DOI:

Tian G B, Wang H N, Zhang A Y, Zhang Y, Fan W Q, Xu C W, Zeng B, Guan Z B, Zou L K. 2012. Detection of clinically important b-lactamases in commensal Escherichia coli of human and swine origin in western China. Journal of Medical Microbiology 61: 233–38. DOI:

Van den Boggard A E and Stobberingh E E. 1999. Antibiotic usage in animals: impact on bacterial resistance and public health. Drugs 58: 589–607. DOI:









How to Cite

BEGUM, J., DUTTA, T. K., CHOUDHARY, P. R., CHANDRA, R., & VARTE, Z. (2015). Antimicrobial assay of shigatoxigenic E. coli (STEC) and enteropathogenic E. coli (EPEC) isolated from diarrhoeic faecal samples of piglets and infants in Mizoram. The Indian Journal of Animal Sciences, 85(10), 1067–1072.