Phylogenic diversity analysis of rumen acetogens in adult sheep fed on conventional roughage diet


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Authors

  • P K MALIK Senior Scientist, National Institute of Animal Nutrition and Physiology, Bengaluru, Karnataka 560 030 India
  • A THULASI Senior Scientist, National Institute of Animal Nutrition and Physiology, Bengaluru, Karnataka 560 030 India
  • N M SOREN Senior Scientist, National Institute of Animal Nutrition and Physiology, Bengaluru, Karnataka 560 030 India
  • L JOSE Ph.D. Scholar, National Institute of Animal Nutrition and Physiology, Bengaluru, Karnataka 560 030 India
  • K S PRASAD Principal Scientist, National Institute of Animal Nutrition and Physiology, Bengaluru, Karnataka 560 030 India
  • C S PRASAD Former Director, National Institute of Animal Nutrition and Physiology, Bengaluru, Karnataka 560 030 India

https://doi.org/10.56093/ijans.v85i10.52414

Keywords:

fhs gene, Molecular characterization, Phylogenic diversity, Rumen acetogens, Sheep

Abstract

The study was conducted to screen the Indian sheep for the presence as well as diversity of rumen acetogens. Rumen liquor samples from 3 adult female sheep (Mandya x Rambouillet) was collected through stomach tube. Genomic DNA was isolated and amplified using touchdown PCR protocol for partial formyltetrahydrofolate synthetase (fhs) gene sequence. Bioinformatic analysis of obtained sequences was done to construct phylogenic tree for molecular characterization and diversity analysis. PCR based amplification and further agarose gel electrophoresis revealed the presence of fhs functional gene in sheep rumen confirming the occurrence of acetogens in adult sheep fed on conventional roughage diet. However, we could not amplify the gene acs conserved with acetogens described by Australian workers. From the phylogenic tree it is clear that act1 group is distant from all other clones (act2 - act10) obtained in this study and represent a distinct uncultured acetogens genera. The act3 and act5 clones are also present in different clusters and are distant to act2, act10, act4 and act6 are closer to each other in the phylogenic tree but distant from act1. From the phylogeny tree it is clear that majority of the acetogens in these sheep are different from those acetogens reported elsewhere in the world. Thus, there is an urgent need to screen our livestock for the acetogens species/strains and to culture them for exploring the prospects of their use as alternate H2 sink for methane mitigation from ruminants.

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References

Boccazzi P and Patterson J A. 2011. Using hydrogen limited anaerobic continuous culture to isolate low hydrogen threshold ruminal acetogenic bacteria. Agriculture Food Analytical Bacteriology 1: 33–44.

Gagen E J, Denman S E, Padmanabha J, Zadbuke S, Jassim R Al, Morrison M and McSweeney C S. 2010. Functional gene analysis suggests different acetogen populations in the bovine rumen and tammar wallaby forestomach. Applied and Environmental Microbiology 76: 7785–95. DOI: https://doi.org/10.1128/AEM.01679-10

Henderson G, Naylor G E, Leahy S C and Janssen P H. 2010. Presence of novel, potentially homoacetogenic bacteria in the rumen as determined by analysis of formyltetrahydrofolate synthetase sequences from ruminants. Applied and Environmental Microbiology 76: 2058–66. DOI: https://doi.org/10.1128/AEM.02580-09

Joblin K N. 1999. Ruminal acetogens and their potential to lower ruminant methane emissions. Australian Journal of Agricultural Research 50: 1307–13. DOI: https://doi.org/10.1071/AR99004

Leaphart A B and Lovell C R. 2001. Recovery and analysis of formyltetrahydrofolate synthetase gene sequences from natural populations of acetogenic bacteria. Applied and Environmental Microbiology 67: 1392–95. DOI: https://doi.org/10.1128/AEM.67.3.1392-1395.2001

Malik P K, Singhal K K, Deshpande S B and Siddique R A. 2012. Mitigation strategies for enteric methane emission with special reference to biological approaches: a review. Indian Journal of Animal Sciences 82 (8): 794–804.

Malik P K, Soren N M, Thulasi A, Jose L and Prasad C S. 2014. Simple method for rumen content collection from 2 days old lambs. Indian Veterinary Journal 92: 46–48.

Matsui H, Kojima N and Tajima K. 2008. Diversity of the formyltetrahydrofolate synthetase gene (fhs) a key enzyme for reductive acetogenesis in the bovine rumen. Bioscience, Biotechnology and Biochemistry 72: 3273–76. DOI: https://doi.org/10.1271/bbb.70375

Morrison K. 2009. Towards greener grazing. Nature Reports Climate Change (3): 104–106. Available at : http://www.nature.com/climate/2009/0909/full/climate.2009.85.html DOI: https://doi.org/10.1038/climate.2009.85

Ranjhan S K. 1998. Nutrient Requirements of Livestock and Poultry. 2nd edn, pp 72. Indian Council of Agricultural Research, New Delhi.

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Submitted

2015-10-07

Published

2015-10-07

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How to Cite

MALIK, P. K., THULASI, A., SOREN, N. M., JOSE, L., PRASAD, K. S., & PRASAD, C. S. (2015). Phylogenic diversity analysis of rumen acetogens in adult sheep fed on conventional roughage diet. The Indian Journal of Animal Sciences, 85(10), 1104–1107. https://doi.org/10.56093/ijans.v85i10.52414
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