Comparative evaluation of agglutination assay with microscopy and polymerase chain reaction for detection of Trypanosoma evansi in bovines of Punjab

L D SINGLA; AMRITA SHARMA; PARAMJIT KAUR; M S BAL

doi:10.56093/ijans.v85i11.53038

Authors

L D SINGLA Professor-cum-Head, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
AMRITA SHARMA Senior Research Fellow, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
PARAMJIT KAUR Assistant Professor, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
M S BAL Assistant Professor, Department of Veterinary Parasitology, College of Veterinary Sciences

https://doi.org/10.56093/ijans.v85i11.53038

Keywords:

Bovines, CATT, PCR, Punjab, Trypanosoma evansi

Abstract

Present investigation was aimed to evaluate the diagnostic efficacy of CATT/T.evansi in comparison to Giemsa stained thin blood smear examination and PCR for detection of bovine trypanosomosis in Punjab. Analysis of 264 blood and sera samples revealed 1.89% (5/264), 34.47% (91/264) and 51.89% (137/264) positivity by GSTBS, CATT/T. evansi and PCR, respectively, for Trypanosoma evansi. Sensitivity of CATT/T. evansi was 100% and 30.65% as compared to GSTBS and PCR, while its specificity was 66.79% and 61.42%, respectively. PCR is more sensitive and specific, and is able to detect the latent infections, however, it is time and cost ineffective technique. CATT/T. evansi is a quick and easy pen-site test suitable for mass screening of T. evansi endemic in developing countries.

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