Comparative evaluation of agglutination assay with microscopy and polymerase chain reaction for detection of Trypanosoma evansi in bovines of Punjab


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Authors

  • L D SINGLA Professor-cum-Head, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
  • AMRITA SHARMA Senior Research Fellow, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
  • PARAMJIT KAUR Assistant Professor, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
  • M S BAL Assistant Professor, Department of Veterinary Parasitology, College of Veterinary Sciences

https://doi.org/10.56093/ijans.v85i11.53038

Keywords:

Bovines, CATT, PCR, Punjab, Trypanosoma evansi

Abstract

Present investigation was aimed to evaluate the diagnostic efficacy of CATT/T.evansi in comparison to Giemsa stained thin blood smear examination and PCR for detection of bovine trypanosomosis in Punjab. Analysis of 264 blood and sera samples revealed 1.89% (5/264), 34.47% (91/264) and 51.89% (137/264) positivity by GSTBS, CATT/T. evansi and PCR, respectively, for Trypanosoma evansi. Sensitivity of CATT/T. evansi was 100% and 30.65% as compared to GSTBS and PCR, while its specificity was 66.79% and 61.42%, respectively. PCR is more sensitive and specific, and is able to detect the latent infections, however, it is time and cost ineffective technique. CATT/T. evansi is a quick and easy pen-site test suitable for mass screening of T. evansi endemic in developing countries.

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2015-11-02

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2015-11-02

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SINGLA, L. D., SHARMA, A., KAUR, P., & BAL, M. S. (2015). Comparative evaluation of agglutination assay with microscopy and polymerase chain reaction for detection of Trypanosoma evansi in bovines of Punjab. The Indian Journal of Animal Sciences, 85(11), 1164–1166. https://doi.org/10.56093/ijans.v85i11.53038
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