Identification of differentially expressed proteins in the testes of normal yaks and sterile hybrids by two-dimensional electrophoresis and mass spectrometry

WEI FU; CAIXIA LI; WENJING LIU; XIAOQIN MA; XIANG QIU; LIANG REN; LIN HUANG; SUYU JIN; YUCAI ZHENG

doi:10.56093/ijans.v85i11.53080

Authors

WEI FU Research fellow, Southwest University for Nationalities, Chengdu 610 041 China
CAIXIA LI Research fellow, Southwest University for Nationalities, Chengdu 610 041 China
WENJING LIU Lecturer, School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan
XIAOQIN MA Research fellow, Southwest University for Nationalities, Chengdu 610 041 China
XIANG QIU Senior engineer, Southwest University for Nationalities, Chengdu 610 041 China
LIANG REN Research fellow, Southwest University for Nationalities, Chengdu 610 041 China
LIN HUANG Engineer, Southwest University for Nationalities, Chengdu 610 041 China
SUYU JIN Senior engineer, Southwest University for Nationalities, Chengdu 610 041 China
YUCAI ZHENG Professor, College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610 041 China

https://doi.org/10.56093/ijans.v85i11.53080

Keywords:

Bos grunniens, Hybrid, Male sterility, Testis, Two-dimensional electrophoresis

Abstract

The study was conducted to compare differentially expressed proteins in the testes of adult yaks (Bos grunniens) and sterile cattle-yaks, in order to elucidate mechanisms of hybrid male sterility. Total proteins were extracted from testes and subject to 2-dimensional electrophoresis and mass spectrometry identification. A total of 19 differentially expressed proteins were identified, of which 13 were downregulated and 4 upregulated in cattle-yak testes. Interestingly, two chaperones (T-complex protein 1 and peptidyl-prolyl cis-trans isomerase) with decreased expressions in the testes of cattle-yaks were revealed, however, their association with the hybrid sterility is unclear. Protein phosphatase methylesterase-1 was decreased by over 8-folds in the testes of cattle-yaks than yaks, and may be associated with cell cycle arrest during spermatogenesis. Several proteins involved in male fertility and/or energy metabolism were also identified, such as mitochondrial peroxiredoxin-5 and sorbitol dehydrogenase. In addition, N (G), N (G)-dimethylarginine dimethylaminohydrolase (DDAH), which can increase the production of nitric oxide and affect normal spermatogenesis in testis, was upregulated in cattle-yak testes. The increased expression of DDAH is probably associated with male sterility of cattle-yaks. The results of this study suggest that the mechanisms of male sterility of cattle-yaks might be associated with many proteins of diverse functions, and some proteins such as chaperones and testis-specific proteins may play important roles.

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