Expression pattern of OCT 4, NANOG and HSP 90 β gene in oocytes and various stages of in vitro produced sheep embryos


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Authors

  • S RAJA Department of Animal Reproduction, Gynaecology and Obstetrics, Madras Veterinary College
  • S BALASUBRAMANIAN Professor and Head, Veterinary University and Peripheral Hospital, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
  • G DHINAKARRAJ Professor and Head, Department of Animal Biotechnology, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
  • C VEERAPANDIAN Dean, Veterinary College and Research Institute, Orathanadu, Thanjavur

https://doi.org/10.56093/ijans.v85i11.53143

Keywords:

Genes, In vitro fertilization, Parthenogenetic activation, Quantitative real time PCR, Sheep

Abstract

The study was conducted to assess the expression pattern of pluripotent genes (OCT 4 and NANOG) and stress response gene HSP 90 ß by quantitative real time polymerase chain reaction (qRT-PCR) in immature and mature oocytes and 2 cell, 4 cell, morula stages of in vitro fertilized (IVF) and parthenogenetically activated (PA) preimplantation ovine embryos to investigate the similarity between them in turn for the establishment of embryonic stem cells (ESCs) from parthenote embryos. The pluripotent related genes showed almost similar pattern of expression between IVF and PA embryos at all the developmental stages which indicated that PA embryos could be used as an alternate source for the production of embryonic stem cells. The stress response gene showed increased levels after in vitro maturation and non significant differences between IVF and PA embryos, thus, indicating no significant differences between the quantum of stress experienced by the 2 groups of embryos which would not affect the development of embryo quality and in turn affect the parthenote embryonic stem cells (PESCs).

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References

Bebbere D, Bogliolo L, Ariu F, Fois S, Leoni G G, Succu S, Berlinguer F and Ledda S. 2010. Different temporal gene expression patterns for ovine pre-implantation embryos produced by parthenogenesis or in vitro fertilization. Theriogenology 74 (5): 712–23. DOI: https://doi.org/10.1016/j.theriogenology.2010.03.024

Goossens K., Poucke M V, Soom A V, Vandesompele J O, Zeveren A V and Peelman L J. 2005. Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos. BMC Developmental Biology 5 (27): 1–9. DOI: https://doi.org/10.1186/1471-213X-5-27

Hao J, Zhu W W, Sheng C, Yang Y and Qi Z. 2009. Human parthenogenetic embryonic stem cells: one potential resource for cell therapy. Science China Ser C-Life Science 52 (7): 599– 02. DOI: https://doi.org/10.1007/s11427-009-0096-2

Kobayashi K, Yamashita S and Hoshi H. 1994. Influence of epidermal growth factor and transforming growth factor-on in vitro maturation of cumulus cell enclosed bovine oocytes in a defined medium. Journal of Reproduction and Fertility 100: 439–46 DOI: https://doi.org/10.1530/jrf.0.1000439

Kumar D, Anand T, Shah R A , Singh M K, Chauhan M S and Manik R S. 2012. Expression patterns of oct–4 and nanog genes in buffalo (Bubalus bubalis) embryos produced by in vitro fertilization or parthenogenetic activation. Indian Journal of Animal Sciences 82 (8): 834–37

Liu N, Enkemann S. A, Liang P, Hersmus R, Zanazzi C, Huang J, Wu C, Chen Z, Looijenga L H J, Keefe D L and Liu L. 2010. Genome-wide gene expression profiling reveals abberant MAPK and Wnt signaling pathways associated with early parthenogenesis. Journal of Molecular and Cell Biology 2 (6): 318–32. DOI: https://doi.org/10.1093/jmcb/mjq029

Parrish J J, Susko-Parrish J, Winer M A and First N L. 1988. Capacitation of bovine sperm by heparin. Biology of Reproduction 38: 1171–80. DOI: https://doi.org/10.1095/biolreprod38.5.1171

Rodriguez-Gonzalez E, Lopez-Bejar M, Vellila E and Paramio M T. 2002. Selection of prepubertal goat oocytes using the brilliant cresyl blue test. Theriogenology 57: 1397– 09. DOI: https://doi.org/10.1016/S0093-691X(02)00645-3

Wani N A, Wani G M, Khan M Z and Salahudin S. 2000. Effect of oocyte harvesting technique on in vitro maturation and in vitro fertilization in sheep. Small Ruminant. Research 36: 63– 67. DOI: https://doi.org/10.1016/S0921-4488(99)00097-8

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Submitted

2015-11-04

Published

2015-11-04

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How to Cite

RAJA, S., BALASUBRAMANIAN, S., DHINAKARRAJ, G., & VEERAPANDIAN, C. (2015). Expression pattern of OCT 4, NANOG and HSP 90 β gene in oocytes and various stages of in vitro produced sheep embryos. The Indian Journal of Animal Sciences, 85(11), 1187–1189. https://doi.org/10.56093/ijans.v85i11.53143
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