Role of calcium and magnesium administration on sex ratio skewing, follicular fluid protein profiles and steroid hormone level and oocyte transcripts expression pattern in Wistar rat


Abstract views: 194 / PDF downloads: 12

Authors

  • A ARANGASAMY Senior Scientist, ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, Karnataka 560 030 India
  • S SELVARAJU Senior Scientist, ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, Karnataka 560 030 India
  • S PARTHIPAN Ph.D Scholar, ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, Karnataka 560 030 India
  • L SOMASHEKAR Ph.D Scholar, ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, Karnataka 560 030 India
  • D RAJENDRAN Senior Scientist, Animal Nutrition Division, ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, Karnataka 560 030 India
  • J P RAVINDRA Principal Scientist and Head, Animal Physiology Division, ICAR-National Institute of Animal Nutrition and Physiology, Bengaluru, Karnataka 560 030 India

https://doi.org/10.56093/ijans.v85i11.53227

Keywords:

Calcium, Magnesium, Reproduction, Sex ratio, Testosterone, Wistar rat

Abstract

An attempt was made with 2% calcium and 0.4% magnesium administration to assess the changes in sex ratio skewing in Wistar rat. The results indicated that there was a significant change in sex ratio skewing towards females (11.7% and sex ratio skewed was 1:1.3). There was a significant reduction in serum testosterone levels (P< 0.01) between control (0.35±0.04 ng/ml) and treatment (0.20±0.08 ng/ml) groups and a nonsignificant decrease in follicular fluid. Significant changes were observed in serum T4 level between control (52.32±2.74 nmol/L) and treatment (33.18±3.75 nmol/L) groups. Variation in follicular fluid protein profiles were observed by 2D gel electrophoresis. Differential expression of protein with high molecular weight (52 and 80 kDa) and low pH is not present in treatment group and protein with low molecular weight (10 and 11 kDa) and high pH were observed in treatment group. The expression of transcripts for BMP-15 was 11.5 folds higher and GDF-9 was 3.5 folds higher in treatment group than control. Our results suggested that possible mechanism to favour female offspring could be reduction in serum testosterone and lowering follicular fluid protein molecular weight along with high pH (haemoglobin subunit beta-1 protein (Mr) 15.9 kDa; cytochrome b-c1 complex subunit 8 (Mr) 9.8 kDa; asparagine synthetase (Mr) 64.7 kDa; metal transporter CNNM4(Mr) 87.4 kDa) and increased mRNA expression of BMP-15 and GDF-9 in oocyte.

Downloads

Download data is not yet available.

References

Arangasamy A, Selvaraju S, Nazar S, Somashekar L, Parthipan S, Gowda N K S and Ravindra J P. 2015. Effect of calcium and magnesium administration on serum and follicular fluid mineral and protein profiles in buffaloes. Indian Journal of Animal Sciences 85 (3): 247–49.

Celik K S, Serbest S, Vurur A, Pala K and Daglioglu. 2003. Experiments to investigate the factors that affect the rate of sex constitution. Pakistan Journal of Nutrition 2 (4): 238–41. DOI: https://doi.org/10.3923/pjn.2003.238.241

Chandraju S, Ashraf Beirami C S and Chidan Kumar. 2011. Effect of calcium and magnesium in identification of baby in high sugar mammals. Research in Biotechnology 2 (3): 23–31.

Eppig J J, Pattricia F, Ward-Bailey and Douglas L C. 1985. Hypoxanthine and adenosine in ovarian follicular: Concentration and activity in maintaining oocyte meiotic arrest. Biology of Reproduction 33: 1041–49. DOI: https://doi.org/10.1095/biolreprod33.5.1041

Farhadi N, Khatamsaz S, Shojaeifard M, Kargar-Jahromi and Khabbaz Z. 2014. Effects of administration of hydroalcohholic extract of ginger (Zingiber officinale) on blood serum cations (Mg, Na+, K+, Ca+) and the sex ratio of male and female newborn wister rats. Journal of Biologytodays world 3: 157– 61. DOI: https://doi.org/10.15412/J.JBTW.01030704

Grant V J and Chamley L W. 2010.Can mammalian mothers influence the sex of their offspring peri-conceptually? Reproduction 140 (3): 425–33. DOI: https://doi.org/10.1530/REP-10-0137

Gromova I and Celis J E. 2006. Protein Detection in Gels by Silver Staining: A Procedure Compatible with Mass Spectrometry. Cell Biology. A Laboratory Handbook. Vol. 4, (Eds) Celis J E, Carter N, Hunter T, Shotton D, Simons K and Small J V. Academic Press, San Diego, CA. DOI: https://doi.org/10.1016/B978-012164730-8/50212-4

Khalifa E I, Ahmed M E, Abdel-Salaam I, El-Zelaky O A and Abdel-Gawad A M. 2009. Effect of semen extender pH value on Rahmany ram characteristics and on altering sex ratio of offspring. Agricultural Research Journal, Suez Canal University 9 (1): 49–52.

Pfaffl M W, Horgan G W and Dempfle L. 2002. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real time PCR. Nucleic Acids Research 30: e36. DOI: https://doi.org/10.1093/nar/30.9.e36

Oliver D Amours, Marlene Fortier G F, Pierre Leclerc and Robert Sullivan. 2010. Proteomic comparison of detergent-extracted sperm proteins from bulls with different fertility indexes. Reproduction 139 (3): 545–56. doi: 10.1530/REP–09–0375. DOI: https://doi.org/10.1530/REP-09-0375

Rosenfeld C S and Roberts R M. 2004. Maternal diet and other factors affecting offspring sex ratio: a review. Biology of Reproduction 71: 1063 70. DOI: https://doi.org/10.1095/biolreprod.104.030890

Sheldon B C and West S A. 2004. Maternal dominance, maternal condition, and offspring sex ratio in ungulate mammals. American Naturalist 163: 40–54. DOI: https://doi.org/10.1086/381003

Snedecor G W and Cochran G W. 1989. Statistical Methods. 6th edn. Iowa State University Press, USA.

Sosic-Jurjevic B, Filipovic M, Manojlovic Stojanoski and Sekulic. 2006. Calcium administration decreases thyroid functioning in middle-ages female rats. Achieves of Biological Sciences Belgrade 58: 31P–32P. DOI: https://doi.org/10.2298/ABS060431PS

Trivers R L and Willard D E. 1973. Natural selection of parental ability to vary the sex ratio of offspring. Science 179: 90–92. DOI: https://doi.org/10.1126/science.179.4068.90

Vahidi A R and Sheikhha M H. 2007. Comparing the effects of sodium and potassium diet with calcium and magnesium diet on sex ratio of rats offspring. Pakistan Journal of Nutrition 6: 44–48. DOI: https://doi.org/10.3923/pjn.2007.44.48

Vanessa Porto Machado1, Rômulo José Vieira, José Ferreira Nunes Arlindo de Alencar Araripe Moura. 2012. Sex ratio after insemination using bovine sperm re-diluted with powdered coconut water extender (PCW-111®). Ciência Animal - Suplemento, 2012.

Downloads

Submitted

2015-11-05

Published

2015-11-05

Issue

Section

Articles

How to Cite

ARANGASAMY, A., SELVARAJU, S., PARTHIPAN, S., SOMASHEKAR, L., RAJENDRAN, D., & RAVINDRA, J. P. (2015). Role of calcium and magnesium administration on sex ratio skewing, follicular fluid protein profiles and steroid hormone level and oocyte transcripts expression pattern in Wistar rat. The Indian Journal of Animal Sciences, 85(11), 1190–1194. https://doi.org/10.56093/ijans.v85i11.53227
Citation