A novel multiplex PCR assay for simultaneous detection of three important pathotypes of Escherichia coli from diarrhoeic piglets


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Authors

  • S RAJKHOWA Senior Scientist, National Research Centre on Pig (ICAR), Rani, Asom 781 131 India
  • D K SARMA Director, National Research Centre on Pig (ICAR), Rani, Asom 781 131 India
  • I SHAKUNTALA Principal Scientist, Animal Health Division, ICAR Research Complex for NEH Region, Umiam, Meghalaya
  • N R SAHOO Scientist, Division of Animal Genetics & Breeding, IVRI, Izatnagar

https://doi.org/10.56093/ijans.v85i11.53260

Keywords:

Diarrhoea, Escherichi coli pathotypes, Novel m-PCR, Piglets, Rapid detection

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References

Gunzburg S T, Tornieporth N G and Riley L W. 1995. Identification of enteropathogenic Escherichia coli by PCRbased detection of the bundle-forming pilus gene. Journal of Clinical Microbiology 33: 1375–77. DOI: https://doi.org/10.1128/jcm.33.5.1375-1377.1995

Ho W S, Tan L K, Ooi P T, Yeo C C and Thong K L. 2013. Prevalence and characterization of verotoxigenic-Escherichia coli isolates from pigs in Malaysia. BMC Veterinary Research 9: 109. DOI: https://doi.org/10.1186/1746-6148-9-109

Mohlatlole R P, Madoroba E, Muchadeyi F C, Chimonyo M, Kanengoni A T and Dzomba E F. 2013.Virulence profiles of enterotoxigenic, shiga toxin and enteroagg regative Escherichia coli in South African pigs. Tropical Animal Health and Production 45: 1399–405. DOI: https://doi.org/10.1007/s11250-013-0377-4

Rajkhowa S, Kalita C and Sarma D K. 2014. Enterotoxigenic and attaching and effacing Escherichia coli strains in diarrhoeic piglets and their antibiogram. Philippine Journal of Veterinary Medicine 51: 30–37.

Rajkhowa S and Sarma D K. 2014. Prevalence and antimicrobial resistance of porcine O157 and non-O157 Shigatoxinproducing Escherichia coli from India. Tropical Animal Health and Production 46: 931–37. DOI: https://doi.org/10.1007/s11250-014-0587-4

US EPA. 1992. Guidelines for Water Reuse. United States Environmental Protection Agency, Washington. http://water.epa.gov/aboutow/owm/upload/Water-Reuse-Guidelines625r04108.pdf

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Submitted

2015-11-06

Published

2015-11-06

Issue

Section

Short-Communication

How to Cite

RAJKHOWA, S., SARMA, D. K., SHAKUNTALA, I., & SAHOO, N. R. (2015). A novel multiplex PCR assay for simultaneous detection of three important pathotypes of Escherichia coli from diarrhoeic piglets. The Indian Journal of Animal Sciences, 85(11), 1202–1204. https://doi.org/10.56093/ijans.v85i11.53260
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