A novel multiplex PCR assay for simultaneous detection of three important pathotypes of Escherichia coli from diarrhoeic piglets
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Authors
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S RAJKHOWA
Senior Scientist, National Research Centre on Pig (ICAR), Rani, Asom 781 131 India
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D K SARMA
Director, National Research Centre on Pig (ICAR), Rani, Asom 781 131 India
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I SHAKUNTALA
Principal Scientist, Animal Health Division, ICAR Research Complex for NEH Region, Umiam, Meghalaya
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N R SAHOO
Scientist, Division of Animal Genetics & Breeding, IVRI, Izatnagar
Keywords:
Diarrhoea, Escherichi coli pathotypes, Novel m-PCR, Piglets, Rapid detectionDownloads
References
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Ho W S, Tan L K, Ooi P T, Yeo C C and Thong K L. 2013. Prevalence and characterization of verotoxigenic-Escherichia coli isolates from pigs in Malaysia. BMC Veterinary Research 9: 109. DOI: https://doi.org/10.1186/1746-6148-9-109
Mohlatlole R P, Madoroba E, Muchadeyi F C, Chimonyo M, Kanengoni A T and Dzomba E F. 2013.Virulence profiles of enterotoxigenic, shiga toxin and enteroagg regative Escherichia coli in South African pigs. Tropical Animal Health and Production 45: 1399–405. DOI: https://doi.org/10.1007/s11250-013-0377-4
Rajkhowa S, Kalita C and Sarma D K. 2014. Enterotoxigenic and attaching and effacing Escherichia coli strains in diarrhoeic piglets and their antibiogram. Philippine Journal of Veterinary Medicine 51: 30–37.
Rajkhowa S and Sarma D K. 2014. Prevalence and antimicrobial resistance of porcine O157 and non-O157 Shigatoxinproducing Escherichia coli from India. Tropical Animal Health and Production 46: 931–37. DOI: https://doi.org/10.1007/s11250-014-0587-4
US EPA. 1992. Guidelines for Water Reuse. United States Environmental Protection Agency, Washington. http://water.epa.gov/aboutow/owm/upload/Water-Reuse-Guidelines625r04108.pdf
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