Genetic diversity analysis of Ghoongroo pig based on microsatellite markers

NIHAR RANJAN SAHOO; NASHIMUN NESA; SOUMEN NASKAR; SANTANU BANIK; PRABHAT KUMAR PANKAJ

doi:10.56093/ijans.v85i11.53272

Authors

NIHAR RANJAN SAHOO Scientist, ICARIndian Veterinary Research Institute, Izatnagar
NASHIMUN NESA Research Fellow, Manipur University
SOUMEN NASKAR Scientist, ICAR-National Research Centre on Pig, Rani, Asom 781 311 India
SANTANU BANIK Senior Scientist, ICAR-NRC on Pig. ICAR-National Research Centre on Pig, Rani, Asom 781 311 India
PRABHAT KUMAR PANKAJ Senior Scientist, ICAR-Central Research Institute for Dry land Agriculture, Hyderabad

https://doi.org/10.56093/ijans.v85i11.53272

Keywords:

Genetic variation, Ghoongroo pig, Microsatellite markers, Population bottleneck

Abstract

An attempt was made to measure the genetic variation available within Ghoongroo pigs, a prized germplasm in terms of high growth and multiplication rate among the indigenous varieties, using 22 FAO-ISAG microsatellite markers. The data were analyzed applying different software to estimate the various measures of genetic diversity. The average number of observed allele was 8.18±0.62 and the effective average number of allele was 4.39±0.26. The observed heterozygosity ranged from 0.4 to 0.96 and the expected heterozygosity from 0.55 to 0.85. Average PIC value ranged from 0.55 (S0026) to 0.85 (S0068) with the average value 0.75±0.02. The mean F value was 0.07±0.04. There was no genetic bottleneck observed in the studied population. A total of 45 private alleles were observed which can be used as breed specific markers. The results suggested that all the microsatellite markers were highly polymorphic and suitable for molecular characterization of Ghoongroo pig. There was substantial genetic variation and polymorphism across the studied loci. The low inbreeding observed was a favourable parameter to formulate the appropriate breeding strategies to enhance heterozygosity in the population.

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