Microsatellite analysis after long term selection for egg production in Rhode Island Red chicken

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  • ANANTA KUMAR DAS Subject Matter Specialist (Animal Science), Howrah Krishi Vigyan Kendra, Jagatballavpur, Howrah, West Bengal
  • SANJEEV KUMAR Principal Scientist, Avian Genetics and Breeding Division, Central Avian Research Institute, Izatnagar, Uttar Pradesh 243 122 India
  • ABDUL RAHIM Ph.D. Scholar, Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar



Allele frequency, Effective number of alleles, Line-specific alleles, Microsatellites, RIR chicken lines, Shannon’s information index


This investigation aimed to analyze microsatellites after long term selection for egg production in the selected line (RIRS) of Rhode Island Red chicken and its control line (RIRC) maintained at the institute. Genomic DNA samples isolated from 24 randomly selected birds of RIRS and RIRC line were investigated at 24 microsatellite loci. Microsatellite alleles were separated on 6% urea-PAGE and their sizes were estimated with the help of Gel Doc 2000 system. Allelic data was analyzed. Analysis revealed 2 to 7 alleles in RIRS and 2 to 9 alleles in RIRC line across 24 loci with their sizes ranged from 84 to 276 bp. Observed number of alleles per locus was 4.04±0.23 in RIRS and 4.42±0.33 in RIRC. Allele frequency ranged from 0.083 to 0.667 in RIRS and 0.042 to 0.833 in RIRC. Approximately 34.02% of alleles in RIRS and 39.62% alleles in RIRC were line specific. The frequencies of the specific alleles ranged from 0.083 to 0.667 in RIRS and 0.083 to 0.883 in RIRC. Line specific alleles with higher frequencies can be used in line identification. Corresponding effective number of alleles and Shannon's information index averaged 3.32±0.19 and 1.25±0.06 in RIRS and 3.66±0.32 and 1.30±0.08 in RIRC. These diversity estimates indicated that the control line was more diverse than the selected line and certain specific microsatellite alleles were getting fixed in the selected line.


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How to Cite

DAS, A. K., KUMAR, S., & RAHIM, A. (2015). Microsatellite analysis after long term selection for egg production in Rhode Island Red chicken. The Indian Journal of Animal Sciences, 85(11), 1220–1224. https://doi.org/10.56093/ijans.v85i11.53280