Microsatellite analysis after long term selection for egg production in Rhode Island Red chicken

ANANTA KUMAR DAS; SANJEEV KUMAR; ABDUL RAHIM

doi:10.56093/ijans.v85i11.53280

Authors

ANANTA KUMAR DAS Subject Matter Specialist (Animal Science), Howrah Krishi Vigyan Kendra, Jagatballavpur, Howrah, West Bengal
SANJEEV KUMAR Principal Scientist, Avian Genetics and Breeding Division, Central Avian Research Institute, Izatnagar, Uttar Pradesh 243 122 India
ABDUL RAHIM Ph.D. Scholar, Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar

https://doi.org/10.56093/ijans.v85i11.53280

Keywords:

Allele frequency, Effective number of alleles, Line-specific alleles, Microsatellites, RIR chicken lines, Shannonâ€™s information index

Abstract

This investigation aimed to analyze microsatellites after long term selection for egg production in the selected line (RIR^S) of Rhode Island Red chicken and its control line (RIR^C) maintained at the institute. Genomic DNA samples isolated from 24 randomly selected birds of RIR^S and RIR^C line were investigated at 24 microsatellite loci. Microsatellite alleles were separated on 6% urea-PAGE and their sizes were estimated with the help of Gel Doc 2000 system. Allelic data was analyzed. Analysis revealed 2 to 7 alleles in RIR^S and 2 to 9 alleles in RIR^C line across 24 loci with their sizes ranged from 84 to 276 bp. Observed number of alleles per locus was 4.04±0.23 in RIR^S and 4.42±0.33 in RIR^C. Allele frequency ranged from 0.083 to 0.667 in RIR^S and 0.042 to 0.833 in RIR^C. Approximately 34.02% of alleles in RIR^S and 39.62% alleles in RIR^C were line specific. The frequencies of the specific alleles ranged from 0.083 to 0.667 in RIR^S and 0.083 to 0.883 in RIR^C. Line specific alleles with higher frequencies can be used in line identification. Corresponding effective number of alleles and Shannon's information index averaged 3.32±0.19 and 1.25±0.06 in RIR^S and 3.66±0.32 and 1.30±0.08 in RIR^C. These diversity estimates indicated that the control line was more diverse than the selected line and certain specific microsatellite alleles were getting fixed in the selected line.

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