Prevalence of virulence associated genes in Pasteurella multocida isolates from Tamil Nadu

K RAGAVENDHAR; A THANGAVELU; J JOHN KIRUBAHARAN; B SAMUEL MASILAMONI RONALD; P RAMESH KUMAR; N DANIEL JOY CHANDRAN

doi:10.56093/ijans.v85i12.54362

Authors

K RAGAVENDHAR Senior Research Fellow, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
A THANGAVELU Professor, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
J JOHN KIRUBAHARAN Professor, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
B SAMUEL MASILAMONI RONALD Associate Professor, Vaccine Research Centre - Bacterial Vaccines, Madhavaram Milk Colony, Chennai
P RAMESH KUMAR MRC of CMFRI, Mandapam, Ramnad District, Tamil Nadu
N DANIEL JOY CHANDRAN Professor and Head, Department of Veterinary Microbiology, Madras Veterinary College

https://doi.org/10.56093/ijans.v85i12.54362

Keywords:

Goat, Pasteurella multocida, phfA, Virulence typing

Abstract

Pasteurella multocida is an important pathogen responsible for wide range of diseases in different hosts. In this study, 14 P.multocida isolates from cattle, goat, rabbit and avian origin were examined for capsular type and 11 virulence associated genes using previously described multiplex PCR. Of the 14 isolates, 11 were capsular type A and 3 were capsular type B. Among the virulence associated genes, oma87, hgbA, nanB and sodC were detected in 100% of the isolates and none of the isolates harboured toxA. All the isolates from goat origin showed positive (100%) for phfA gene. Occurrence of filamentous haemagglutinin encoding phfA gene from isolates of goat origin is first reported in this study.

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